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V Vylchev

Publications and source records attributed to V Vylchev.

4 recordsLinked to original sources
PubMed1978

[Experimental immunologic study of a protective anthrax preparation].

Rabbits and sheep immunized with protective anthrax preparation and live anthrax vaccines were examined. Protective anthrax preparation caused changes characteristic of general immunomorphology and immunomorphological reaction of the humoral type. A marked resistance in infection with the virulent anthrax culture was revealed in the animals immunized with this preparation and in those--with live vaccines. Skin allergic reactions were more intensive in sheep immunized with live Ikhtiman's vaccine; there was no reaction in the animals immunized with the protective preparation.

Animals↗
PubMed1976

[Purification of a protective anthrax preparation].

An attempt was made to standardize conditions for obtaining a protective anthrax preparation. A technological scheme permitting to produce 80 mg of a purified lyophilized protective preparation with the activity of 32 immunizing units was applied. It was accepted that 1 IU was the minimal amount of the preparation which provided a survival with the mean time index of 3.0+/-0.5 of guinea pigs after a double immunization at an interval of 14 days. In vitro 1 IU also represented a minimal amount of the preparation which produced three precipitation lines (characteristic of the preparation) in immunoelectrophoretic analysis against the purified anthrax serum. Usually 1 IU in vivo and in vitro corresponded to 2.5 mg of this preparation.

Animals↗
PubMed1975

[Use of protective anthrax antigen in agglutination reactions].

The latex- and hemagglutination tests were applied to determination of the titres of anthrax antibodies in the sera of 982 immunized sheep. Monodispersive standard colur latex of polymethylmetacrylate and protective anthrax antigen was used in these tests. On the basis of the results of titration of control sera titres of anthrax antibodies over 1:16 in the latex- and hemagglutination tests were accepted as positive. There was revealed no difference between the sera of the immunized animals reacting positively in both tests (d = 1.87 less than delta = 2.94 at P = 0.95). The difference between the positive titres determined in the latex-agglutination (55.78%) and hemagglutination (44.41%) tests was significant (d = 11.37 less than delta = 4.39 at P = 0.95). Consequently, both tests could be used for the purpose of determination of anthrax antibody titres, but the latex-agglutination test was more sensitive of the two.

Animals↗