PubMed Health⌕ Search

Biomedical subjects

V W Adamkiewicz

Publications and source records attributed to V W Adamkiewicz.

11 recordsLinked to original sources
PubMed1991

Invasion of bacteria in enamel carious lesions.

A review of recent findings concerning enamel carious lesions is presented. This lesion represents the initial phase of dental caries and is characterized by a demineralization of the subsurface enamel caused by acids of the plaque bacteria. Streptococcus mutans has been described as the etiologic agent of the dental caries and the most acidogenic plaque bacteria. Morphological studies have shown an invasion of microorganisms inside the enamel carious lesion. Unfortunately, several technical problems are associated with such studies. The identification of the invading bacteria has not yet been achieved. The future identification of bacteria inside the subsurface enamel lesions will represent an important step in the prevention of the carious progression.

Dental Caries↗
PubMed1988

Immunoglobulins in human dental caries.

The penetration and complement fixation of sIgA, IgM and IgG were studied in advanced human dental caries with double-staining immunofluorescence technique. Immunoglobulins were found in dental plaque and in the superficial layers of the lesions which were exposed to saliva. sIgA was the most frequent, followed by IgG and IgM. IgG had the greatest capacity for penetration into the lesions, followed by IgM and sIgA. IgM was most frequently found in association with complement C3.

Complement C3↗
PubMed1988

Mechanisms of HIV-associated immunosuppression.

HIV antigens were identified in PBL obtained from HIV-positive patients, using IF and IEM. Studies of the phenotype of HIV-containing lymphocytes showed that OKT4+ cells were the principal target of the virus. Approximately 5% of infected cells were multinucleated. Almost all infected and about 30% of uninfected PBL displayed Ab-C3 complexes on the cell surfaces. Sera from HIV-positive patients contained Ab reacting with cell membranes and intracellular structures of PBL from normal subjects, as demonstrated by IEM. The presence of Ig-C3 complexes on a high percentage of HIV-positive- or negative-PBL suggests that the Ab-C-mediated lympholysis may represent a major mechanism of lymphatic tissue destruction in HIV-infected patients.

Autoantibodies↗
PubMed1987

Identification of bacteria in association with immune components in human carious dentin.

Actinomyces viscosus, A. naselundii, Streptococcus mutans serotype "c" and S. mutans serotype "d/g" were identified in human carious dentin using histological and immunofluorescent techniques. A. viscosus was most frequently found in association with patient's immunoglobulins and complement, followed by S. mutans serotype "d/g", S. mutans serotype "c", and A. naeslundii.

Actinomyces↗
PubMed1978

Allergies of the dental pulp.

A review of publications relating to the allergies of the dental pulp is presented. The pulp--especially when inflamed--contains antigens, lymphocytes, plasmocytes, IgG, IgM, IgA, and IgE antibodies, mast cells, histamine, and possibly C3. However, no antibody-mediated allergy (hypersensitivity) of the pulp has yet been demonstrated. On the other hand, two types of cell-mediated allergy--the graft-rejection reaction and contact hypersensitivity--have been reported, as well as autoimmunity.

Animals↗
PubMed1978

[Allergies and dental pulp (author's transl)].

A review of the articles dealing with allergy in the dental pulp is oppered. The dental pulp--furthermore when inflamed--showes antigens, lymphocyetes and plasmocytes, antibodies (IgG, IgA and IgE), mast-cells, histamine and possibly C3. However an hypersensitivity or allergy of immediate type elicited by circulating antibodies has never been demonstrated. In another way, two types of allergies of cellular type have been described in the dental pulp: graft rejection and contact hypersensitivity. Autoimmunity has also just been described.

Animals↗
PubMed1977

[Experimental allergic pulpitis in rabbits].

Ten male, albino rabbits, weighing about 2,5 kg, were sensitized with 0.05% of 1-chloro-2,4-dinitrobenzene (DNCB) in complete Freund's adjuvant. Ten controls received the complete Freund's adjuvant only. Fourteen days later, the animals were submitted to a dental test. The four incisor pulps were exposed under general anesthesia. A sterile cotton pellet with 10-(-4) mg of DNCB was sealed inside the cavity. A skin test was also performed with 0.1% DNCB. The operative trauma was evaluated on five additional control animals. Twenty four hours after the challenge, the animals were killed and their teeth were studied histologically. Three different pulpitis were observed: 1) in animals without DNCB, a traumatic pulpitis with a mild focal necrosis and serous infiltrate; 2) a toxic pulpitis induced by the DNCB in the controls. They were killed and their teeth were studied histologically. Three different kinds of pulpitis induced in sensitized animals. A considerable necrosis is present close to the pulp exposure. This pulpitis is characterized by a marked serous exudate and by an accumulation of lymphocytes, eosinophils and macrophages, either perivascularly or in the pulpal mesenchyme. A modification of the present classification of pulpitis is proposed.

Animals↗
PubMed1977

[Induction of autoallergic pulpitis in rabbit (author's transl)].

Six male rabbits were injected on days 0, 6 and 12, subcutaneously, intraperitoneally and subcutaneously with 0.5 ml/kg of a 20% suspension of homologous dental pulp, in complete Freund's adjuvant. Six controls received complete Freund's adjuvant only. Leukocytes were counted regularly. A skin test was performed on day 18. On day 22, a dental test was performed to study the macrophage migration inhibition "in vivo". On day 23, the animals were killed, the teeth and skin were analysed histologically. The following results were observed in the experimental group: 1) inhibition of growth, 2) leukocytosis and eosinophilia, 3) a positive skin test with a strong lymphocytic infiltration, 4) an inhibition of "macrophage migration", 5) pulpal lesions, numbering 1 to 6 per rabbit, with lymphocytic plasmocytic and eosinophilic perivascular infiltration. These results indicate an experimental auto-allergic pulpitis. The mechanisms of human pulpitis ought to be reexamined in the light of these observations.

Animals↗