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Biomedical subjects

V Zaman

Publications and source records attributed to V Zaman.

At least 19 recordsLinked to original sources
PubMed1992

A survey of Blastocystis in reptiles.

A total of 28 species of reptiles were investigated for Blastocystis using light microscopy and in vitro culture in biphasic egg slant medium. Blastocystis species were detected in 8 (28.6%) of these 28 species in 3 tortoises (Geochelone elephantopus, G. elegans and G. carbonaria), 3 snakes (Boiga dendrophilla, Python reticulatus and Elaphe radiata), 1 crocodile (Crocodylus porosus) and 1 iguana lizard (Cyclura cornuta). The reptilian Blastocystis appeared to be morphologically similar to B. hominis.

Alligators and Crocodiles↗
PubMed1991

A Blastocystis species from the sea-snake, Lapemis hardwickii (Serpentes: Hydrophiidae).

Observations were made on Blastocystis isolated from the sea-snake, Lapemis hardwickii. Exponential growth of the organism was observed between 2 and 4 days of culture. Vacuolated, amoeboid and granular forms were observed in cultures, similar to B. hominis. The optimal growth temperature for the sea-snake Blastocystis was 24 degrees C compared with 37 degrees C for B. hominis. The karyotypic patterns of B. hominis and the sea-snake Blastocystis were studied in the clamped homogeneous electric field (CHEF) technique and found to be different. Based on the above differences, the sea-snake Blastocystis was designated as Blastocystis lapemi sp. nov.

Animals↗
PubMed1991

Scanning electron microscopy of Setaria cervi adult male worms.

Scanning electron microscopy studies of adult Setaria cervi male worms showed the presence of horn-like lateral appendages and characteristic striated bands on the ventral side of the tail. All other features were indistinguishable from other members of the genus Setaria.

Animals↗
PubMed1990

Ultrastructural study of Helicobacter pylori-associated gastritis.

Endoscopic biopsies of antral mucosa from 26 patients with Helicobacter pylori-associated gastritis were studied by electron microscopy (EM). Scanning electron microscopy (SEM) showed clustering of H. pylori in the intercellular areas, being entrapped by the microvilli which were decreased at the sites where the bacilli were seen. The observations of SEM were confirmed by transmission electron microscopy (TEM), which showed adherence of the bacilli to the cell surface, producing cup-shaped depressions in the epithelial cells, and occasionally intracellular infiltration by H. pylori. There were also depletion of mucus granules, degenerative changes, and disruption of intercellular junction complexes of the epithelial cells. Post-treatment biopsies showed complete disappearance of the bacilli, and ultrastructural changes associated with H. pylori infection were resolved.

Campylobacter↗
PubMed1989

Cellular and humoral immune responses in Campylobacter pylori-associated chronic gastritis.

Gastric cellular and humoral immune response investigated by immunoperoxidase staining of 53 antral biopsies showed significant differences in Campylobacter pylori-associated gastritis as compared with non-bacterial chronic gastritis and normal controls. IgA, secretory component, and complement C3 coated bacilli were seen in all cases of active chronic gastritis. C3 was always associated with coating by IgA, IgM, or both, which were rarely seen in gastritis without polymorphonuclear neutrophil infiltration. Intraepithelial mononuclear cellular infiltration was seen in 18 of 26 cases of C. pylori-associated chronic gastritis. The intraepithelial mononuclear cells stained positively for T cells and histiocytes.

Antibody Formation↗
PubMed1987

Scanning electron microscopy of Brugia malayi.

Adults, infective larvae and microfilariae of Brugia malayi have been examined in the scanning electron microscope. In adult males the long spicule is solid with a spatulate anterior end. The small spicule is hollow and has a cup-like structure at the distal end which is covered by a hood. The infective larvae have dome-shaped caudal papillae, each with a gutter-like structure surrounding its base. The microfilariae are annulated with a distinct tail and a conspicuous hook at the cephalic end. These studies show that scanning electron microscopy of filariids can reveal features useful not only for distinguishing between species but also for understanding the functions of various organelles.

Animals↗
PubMed1987

Ultrastructure of Brugia malayi egg shell and its comparison with microfilarial sheath.

Ultrastructure of Brugia malayi egg shell and microfilarial sheath have been compared. They are identical in appearance, thus confirming that the microfilarial sheath is derived from the egg shell. The surface of the egg shell and the microfilarial sheath have distinct electron-dense projections which may have a protective role for the parasite.

Animals↗
PubMed1986

Differentiation of infective larvae of Brugia malayi and Wuchereria bancrofti by scanning electron microscopy.

A comparative scanning electron microscopy of the infective (third stage) larva of Wuchereria bancrofti and Brugia malayi revealed the following features: The lateral caudal papillae in both species are rounded and the terminal papilla is relatively pointed and smaller. The caudal papillae in W. bancrofti are more protruding while in B. malayi they are flattened. The lateral caudal papillae in B. malayi have an indentation around their bases which is absent in W. bancrofti. This is the most useful distinguishing feature and easily recognizable. The oral opening and the cephalic papillae in both the species are similar and not useful for differentiation.

Animals↗
PubMed1984

Scanning electron microscopy of Trichuris trichiura (Nematoda).

Trichuris trichiura was examined in the scanning electron microscope and revealed a double row of sucker-like structures at the anterior part. It is postulated that they may support the attachment of the worm in host tissues. The sheath of the spicule is covered with spikes and the vulval opening is surrounded by an elevated rim-like structure with indentations.

Animals↗
PubMed1982

Mechanism of destruction of Brugia malayi microfilariae in vitro: the role of antibody and leucocytes.

Human and monkey leucocytes in serum from previously patent Brugia malayi infected monkeys adhered to and caused cytotoxicity to microfilariae of B. malayi in vitro. The phenomenon also occurred in human serum from confirmed cases of bancroftian and malayan elephantiasis. Adherence of leucocytes was not seen in serum from uninfected monkeys, monkeys with patent B. malayi infection and serum from rats infected with Breinlia booliati but now amicrofilaraemic. Immune serum failed to promote adherence of leucocytes to L3 of B. malayi. The activity was lost when the serum was either incubated with Protein A-bearing Staphylococcus aureus or heat inactivated at 56 degrees C for 30 min. Light and electron microscopic observations regarding the type of adhering cells and sequence of events following adherence are described.

Animals↗
PubMed1978

Intestinal nematodes of man.

An account of the life cycle and morbidity of the commoner intestinal nematodes is given and methods of control and eradication discussed.

Ancylostomatoidea↗