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Vincenzo Della Mea

Publications and source records attributed to Vincenzo Della Mea.

7 recordsLinked to original sources
PubMed2006

A web-based system for tissue microarray data management.

BACKGROUND: Tissue Microarray is a novel technique for analysing large amounts of immunohistochemically stained specimens. However, those large amounts make it difficult to design, prepare and analyze a tissue microarray, so that software support is almost inevitable. METHODS: We designed a tissue microarray data management system starting from specifications obtained by pathologists, and arranged for a preliminary validation in thyroid pathology. RESULTS: A web-based system has been developed, basing on open-source software and principles, that was well accepted by pathologists and allowed to carry out a study on 52 thyroid pathology cases. CONCLUSION: Though limited in functionalities, the developed system is effective and can be downloaded at the address http://mitel.dimi.uniud.it/timan/.

Journal Article↗
PubMed2006

User attitudes in analyzing digital slides in a quality control test bed: a preliminary study.

The pathologist examines suitably stained glass slides through a bright field microscope in order to render histopathological or cytological diagnosis by looking at tissues and cells. Glass slides serve as a permanent record of the patient disease. Over the course of a patient's treatment slides may need to be reviewed at other institutions before treatment can commence. Due to their fragile nature a transportable permanent digital facsimile of the glass slide would be ideal. A digital slide is a set of digital images representing the whole slide normally used by the pathologist, or a significant part of it; it is usually made by a large amount of images, up to thousands, which makes its management difficult. The present paper provides a description of the requirements needed to reproduce glass slides and of the available technological equipment, then the features of the two systems we implemented on different hardware are described, together with those of the digital slide viewer. The viewer was evaluated in two experimental test phases, during which user behaviour and diagnostic reports were measured. Digital slides used in the two experiments were acquired with either system. Possible applications of digital slides are then discussed, including undergraduate and professional education, quality control, and image analysis on full samples as well as on tissue microarrays.

Attitude↗
PubMed2005

A pixel-based autofocusing technique for digital histologic and cytologic slides.

The present paper describes a method for autofocusing specifically studied for the acquisition of digital slides, i.e. full histologic and cytologic slides, utilising low-cost equipment. At first, experimentations with some of the most used focus measures and algorithms have been made, in order to choose the most suitable for histologic and cytologic images. Then, a study of the specific features of digital slides has been preliminarily carried out in order to understand the constraints of the domain. These included the capability of autofocusing in an unattended way on thousands of microscope fields, while fast performance is not a strict requirement. Based on the findings, an algorithm based on a dynamic focus position space, with a variance-based focus measure has been adapted to the specific situation. A qualitative and quantitative evaluation of the proposed algorithm allowed us to show that the proposed algorithm is suitable for the acquisition of digital slides, and furthermore it can be implemented starting from a basic microscope with an inexpensive motorised stage. The algorithm is currently implemented into a complete digital slide acquisition system, which is in turn being used for a Quality Assurance Programme in cervicovaginal cytologic screening.

Algorithms↗
PubMed2005

Prerecorded telemedicine.

In prerecorded telemedicine (also known as asynchronous or store-and-forward), the person sending the information and the person receiving it do not need to do so simultaneously; thus, viewing the information can be done at some later time. Prerecorded telemedicine is therefore not appropriate for emergency consultations. In prerecorded telemedicine systems, the following steps can be distinguished: (1) the acquisition of diagnostic information at the remote site; (2) its storage, which can be at either site, or at both; (3) its delivery to the expert site through an appropriate connection; and (4) its display at the expert site. The types of information transferred include audio, data and text, still images and moving images (i.e. video). An increasingly common way of doing prerecorded telemedicine is by email sent via the Internet. Although there are some problems associated with the Internet, its wide availability and low cost have encouraged its use. Examples where email has been used successfully include teleradiology, telecardiology, teledermatology and telepathology. In some situations prerecorded telemedicine is the only way to provide remote medical services, or the most cost-effective method. Clearly, there are also situations when prerecorded telemedicine is not an appropriate way to deliver health services, for example whenever the sender of the information is not qualified to sample the information acquired or the specialist receiving the information must manipulate it, during acquisition, in some way.

Copying Processes↗
PubMed2003

Introducing videoconferencing into educational oncopathology seminars: technical aspects, user satisfaction and open issues.

We used set-top videoconferencing equipment connected by ISDN at 384 kbit/s for six educational seminars held between the University of Udine (the local site) and the National Cancer Institute in Aviano (the remote site), 60 km away. User satisfaction was evaluated by questionnaire. The median length of seminars was 58 min (range 48-61 min), followed by a 20 min (15-26 min) discussion. Eighty-two users answered the questionnaire (a 43% response rate): 56 in Udine (a median of 11 per seminar) and 26 in Aviano (a median of 5 per seminar). Answers to the questions were similar at the two sites. Videoconferencing did not affect the users' experience of attending the seminars, as both interest and clarity were similar at the local and remote site. The results suggested that videoconferencing is a viable method for delivering seminars in oncopathology, where image quality is important.

Adult↗
PubMed2002

Digital storage of glass slides for quality assurance in histopathology and cytopathology.

Proficiency testing programmes for measuring screening skills in pathology are mainly conducted using conventional glass microscope slides. However, the availability of robotic microscopes allows an entire conventional slide to be digitized. Our experiments have shown that, using a widely available robotized microscope and a PC, the image of a single field may be acquired in 2 s on average, including stage movements, autofocus and storage. Digitizing an entire slide, a fully automated procedure, takes up to 8 h. If the image of each field is compressed at an appropriate quality level (a compression ratio of, say, 35:1) it requires about 40 kByte to be stored, resulting in a total storage requirement of about 600 MByte per slide. Thus one CD-ROM can be used to store one virtual slide, as well as a self-installing program to provide a microscope simulator facility. This allows pathologists to examine the virtual case from their computer in a similar manner to looking at a glass slide on a conventional microscope. This permits a new, computer-based approach to proficiency testing in histopathology and cytopathology. Use of virtual slides should encourage the diffusion of national quality assurance programmes, which at present suffer from certain organizational and logistical limitations.

Analog-Digital Conversion↗