Biomedical subjects

Virginie Olive

Publications and source records attributed to Virginie Olive.

2 recordsLinked to original sources

PubMed2006

A role for Phospholipase D in Drosophila embryonic cellularization.

BACKGROUND: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. CONCLUSION: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.

Animals↗

PubMed2005

The spermatogonial stem cell: from basic knowledge to transgenic technology.

Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells. They give rise to the first differentiated spermatogenic cells (spermatogonia). These committed cells will then follow a strictly defined succession of steps, starting with six synchronized mitotic cycles before reaching the first meiotic stages. Following a first identification of the spermatogonial stem cells on morphological and cytological criteria, a functional assay was devised, based on their ability to repopulate the testis of a sterile recipient. Purification and characterization of the stem fraction is in progress. Fundamental knowledge of the biology of the germ line and preclinical studies in several important fields will benefit of these advances, while gene transfer prior to reimplantation opens a new approach in transgenic technology.

Animals↗