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Biomedical subjects

W A Fleming

Publications and source records attributed to W A Fleming.

At least 19 recordsLinked to original sources
PubMed1984

Cellular responsiveness in vitro: the effect of mycobacterial glycopeptides, glycolipids and peptidoglycolipids on the in vitro growth of haemopoietic colony-forming cells.

Incorporation of glycopeptide, glycolipid and peptidoglycolipid preparations from the culture fluid or organisms of a variety of mycobacterial species in suboptimally stimulated cultures of normal mouse bone marrow cells increased the responsiveness of colony-forming cells to colony-stimulating factor, as measured by enhanced colony formation. Possible mechanisms of action and the significance of enhanced colony growth in relation to the mode of action of mycobacterial adjuvants are discussed.

Animals↗
PubMed1979

Hay fever.

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Humans↗
PubMed1976

Cellular responsiveness to stimulation in vitro: increased responsiveness to colony stimulating factor of bone marrow colony-forming cells treated with surface-active agents and cyclic 3'5' AMP.

Addition of low concentrations (10 ng/ml) of saponin or Tween 80 to stimulated cultures of normal mouse bone marrow in agar increased the number of granulocyte-macrophage colonies which developed. Addition of cyclic AMP or dibutyryl cyclic AMP in low concentration (10(-8) to 10(-10) M) also enhanced colony numbers although concentrations above 10(-5) M were inhibitory. enhancement was found when marrow cells were pre-treated with these agents and cultured in their absence. The agents did not stimulate colony development in the absence of colony-stimulating factor and enhancement of colony number occurred only in cultures containing a concentration of colony-stimulating factor which was sub-optimal in terms of maximum colony development. There was no indication of increased colony-stimulating factor production by treated marrow cells under the experimental conditions used to show colony enhancement. It was concluded that the agents caused an increased responsiveness of colony-forming cells to colony-stimulating factor.

Adenosine Monophosphate↗
PubMed1972

Suppression of immune and hemopoietic cellular responses by methisazone.

The antiviral drug methisazone (N-methylisatin beta-thiosemicarbazone) was tested for its effect on immune responses to sheep erythrocytes and on hemopoietic colony-forming cell (granulocyte-macrophage progenitor cell) responses to complete Freund's adjuvant in mice. Suppressive activity was demonstrated in both systems, the immune system being more readily and more consistently susceptible. Evidence is presented which suggests that the insoluble particulate form of the drug has both stimulatory and suppressive effects on the colony-forming cell system, whereas the soluble form is only suppressive. Methisazone increased the mortality from ectromelia in adjuvant-treated animals.

Animals↗
PubMed1970

Cryptococcal meningitis associated with steroid therapy.

Two patients on prolonged steroid therapy developed meningitis due to Cryptococcus neoformans. The first responded satisfactorily to treatment with amphotericin B, both initially and again following relapse. The second died shortly after treatment was begun. Pathogenicity studies suggest that the strain isolated from the fatal case was the more virulent. Cryptococcal meningitis probably occurs more often in Britain than is generally appreciated, and this possibility should be remembered when investigating patients with obscure forms of meningitis; if not, then the correct diagnosis may not be made. Attention is drawn to the increasing number of recently reported cases of this disease which have been associated with long-term steroid therapy.

Adult↗