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Biomedical subjects

W A Nelson-Rees

Publications and source records attributed to W A Nelson-Rees.

At least 37 records · Page 2Linked to original sources
PubMed1979

Establishment of a lymphoblastoid cell line and isolation of an Epstein-Barr-related virus of gorilla origin.

A B-lymphoid cell line was established from a normal gorilla. The cells contained Epstein-Barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. DNA-DNA reassociation kinétics revealed 30 to 40% hybridization to Epstein-Barr virus with 50 genomes per cell. Examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed Herpesvirus gorilla. H. gorilla transformed gibbon B-lymphocytes in vitro.

Animals↗
PubMed1979

Morphological and biochemical properties of a new human breast cancer cell line.

DU4475 is a new human breast cell line derived from a cutaneous metastatic nodule from a patient with advanced breast cancer. It has been in continuous culture for more than 1 year and has survived 140 subcultivations. The cells grow in suspension, displaying clusters and free-floating aggregates with serpentine-like outgrowths. The cell line is hypotetraploid with modal chromosome numbers between 86 and 93 and possesses five to six distinctive marker chromosomes. The cells grow readily in athymic nude mice and produce tumors from which cells can be reintroduced to culture in vitro.

Breast Neoplasms↗
PubMed1978

Neoplastic transformation of canine embryo cells in vitro by N-methyl-N'-nitro-N-nitrosoguanidine.

A cell line derived from a normal beagle embryo was treated in vitro with various levels of N-methyl-N'-nitro-N-nitrosoguanidine or dimethyl sulfoxide (control). Cells treated only with the carcinogen underwent morphologic alteration in vitro, and one of these altered cell lines produced tumors subcutaneously when injected into NIH nude mice. The tumorigenic transformed line formed larger cell aggregates and grew in this aggregate form when suspended in liquid growth medium above an agar base.

Animals↗
PubMed1978

Spontaneous esophageal carcinoma and epithelial cell line of an adult rhesus monkey.

A continuous epithelial cell line, 816A, was established from a lymph node of an adult rhesus monkey with metastatic esophageal carcinoma. These cells are characterized by the presence of desmosomes and a markedly heteroploid karyotype. At a relatively early culture age, electron microscopy showed both budding and extracellular type C virus. Antigen reactive with antisera to Mason-Pfizer monkey virus was observed by complement-fixation. The level of this antigen decreased with increased culture age. To our knowledge, the 816A cells represent the only established simian or human cell line of esophageal carcinoma origin.

Animals↗
PubMed1977

Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses.

Two newly established human bladder carcinoma cell lines, designated HT-1197 and HT-1376, were characterized. Cells of both cultures exhibited fine structural microvilli and tonofibrils indicative of their epithelial origin. In addition, desmosomes were also present in HT-1197. Marker chromosomes present in HT-1197 and HT-1376 distinguished these from each other and from other known human tumor cell lines. Both cultures grew in soft agar, induced fibrinolytic activity, and were tumorigenic in mice and hamsters. No type C or other virus expression was detected in these cell lines nor in other human urothelial tumors tested.

Animals↗
PubMed1977

Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines.

We characterized two human cell lines (Hs578T and Hs578Bst), which provide several unique features that should be useful in the study of breast disease. Hs578T, derived from a carcinosarcoma, is epithelial in origin. Hs578Bst, established from normal tissue peripheral to the tumor, is myoepithelial in origin. This is the first report of companion cell lines, one malignant and one normal, established from the same organ.

Aneuploidy↗
PubMed1976

Alteration of the cell membrane architecture during suspension and monolayer culturing.

Using suspension-growth-adapted HeLa (S3) cells, we followed the alterations in cell morphology during attachment and spreading on a glass surface in vitro by scanning electron microscopy. Suspension cultivation obviated the need for trypsinization or EDTA treatment of the cells before seeding, thus the cell surface was not chemically altered. The suspension-grown cells were sperical and covered with microvilli. Three hours after seeding into stationary culture, attached cells had surface blebs and large processes, but no microvilli. During the following 9 h, the surface topography altered stepwise producing a complex lamelloplasm. The paper discusses how these membrane events are related to the cell cycle and metastasis.

Cell Membrane↗
PubMed1976

The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas.

We have extensively characterized cell lines derived from six human osteosarcomas. The growth properties of these cultures were compared to those of fibroblastic cultures derived from skin of osteosarcoma patients and skin of bone-marrow of normal individuals. Each tumor-derived line showed some but not all of the abnormal growth properties usually associated with malignant transformation, indicating that tumor cells rather than normal stromal cells had in fact been cultured. The parameters measured included saturation density, cell morphology, growth pattern, growth on contact inhibited monolayers of normal fibroblastic or epithelial cells and tumorigenicity in immunosuppressed mice. Although the skin fibroblasts from the osteosarcoma patients appeared normal in vitro, they showed a greater ability to grow in immunosuppressed mice than did normalfibroblasts. This observation suggests that all fibroblasts of osteosarcoma patients may have an increased propensity for malignant transformation.

Adolescent↗
PubMed1976

HeLa cultures defined.

A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa cell contaminants. Eleven additional cell lines not previously indicted are described. When they exhibit (i) type A (fast) mobility for glucose-6-phosphate dehydrogenase, (ii) phosphoglucomutase type 1 at locus 1 and locus 3, (iii) absence of a Y chromosome by fluorescent staining, and (iv) possession of a complex of trypsin-Giemsa banded marker chromosomes present in known HeLa cells, then cell substrates regardless of designation should be considered de facto strains of HeLa.

Cell Line↗