W B Hong | PubMed Health

PubMed1990

Miniaturization of a liquid membrane sensor for the determination of bile acids.

The preparation of a compound liquid ion exchanger used for the miniaturization of bile acid sensitive microelectrode is described. The liquid ion exchanger is 2% benzyldimethylhexadecylammonium cholate in decanol/0.1 M hexadecyltributylammonium taurocholate in 5% hexachlorobenzene, 0.5% bromoacetanilide O-dichlorobenzene. The slopes, detection limits, selectivity coefficients, drifts and response time of the various bile acid sensitive microelectrodes are evaluated and compared.

Bile Acids and Salts↗

PubMedDate not supplied

Two-barrel bile-acids-sensitive microelectrodes based on liquid ion exchanger.

Several liquid membrane microelectrodes sensitive to bile acids (two barrel, tip diameter about 0.5 micron) are described. The results of different liquid ion exchangers such as Aliquat 336/decanol, trioctylmethylammonium/decanol, hexadecyltrimethylammonium/decanol, benzyldimethylhexadecylammonium/decanol, hexadecyltributylammonium/5% hexachlorobenzene + 0.5% bromoacetanilide in o-dichlorobenzene are compared with each other, and the better one among them is the mixture of benzyldimethylhexadecylammonium cholate/decanol with hexadecyltributylammonium taurocholate/5% hexachlorobenzene + 0.5% bromoacetanilide in o-dichlorobenzene because of its quicker response time and low drift. The calibration curves, slopes, test limits, selective coefficients, drifts, and response times of the various bile-acids-sensitive microelectrodes in different calibration solutions were demonstrated and compared with each other.

Bile Acids and Salts↗