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W B NEELY

Publications and source records attributed to W B NEELY.

18 recordsLinked to original sources
PubMed1963

ACTION OF FORMALDEHYDE ON MICROORGANISMS. I. CORRELATION OF ACTIVITY WITH FORMALDEHYDE METABOLISM.

Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. I. Correlation of activity with formaldehyde metabolism. J. Bacteriol. 85:1028-1031. 1963.-The action of formaldehyde in concentrations sublethal for the culture appeared to be at least twofold. The first phase caused a drop in viability, followed by a second phase where bacteriostatic conditions existed. During both these intervals, formaldehyde was being metabolized. As soon as the organism had lowered the formaldehyde concentration to a critical level, the normal growth cycle was resumed, with a resultant increase in cell population.

Carbon Dioxide↗
PubMed1963

ACTION OF FORMALDEHYDE ON MICROORGANISMS. II. FORMATION OF 1,3-THIAZANE-4-CARBOXYLIC ACID IN AEROBACTER AEROGENES TREATED WITH FORMALDEHYDE.

Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. II. Formation of 1,3-thiazane-4-carboxylic acid in Aerobacter aerogenes treated with formaldehyde. J. Bacteriol. 85:1420-1422. 1963.-A culture of Aerobacter aerogenes was found to incorporate C(14)-labeled formaldehyde into 1,3-thiazane-4-carboxylic acid. The reaction was found to proceed nonenzymatically from homocysteine and formaldehyde. As a consequence of this reaction, the ability of homocysteine to counteract the effects of formaldehyde on A. aerogenes was investigated.

Carboxylic Acids↗
PubMed1963

ACTION OF FORMALDEHYDE ON MICROORGANISMS. III. BACTERICIDAL ACTION OF SUBLETHAL CONCENTRATIONS OF FORMALDEHYDE ON AEROBACTER AEROGENES.

Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. III. Bactericidal action of sublethal concentrations of formaldehyde on Aerobacter aerogenes. J. Bacteriol. 86:445-448. 1963.-Bacteriostatic concentrations of formaldehyde were found to be bactericidal during the initial growth period. This bactericidal activity was due to the primary induction of unbalanced growth by formaldehyde. The subsequent formation of 1,3-thiazane-4-carboxylic acid prevented the synthesis of methionine. This dual activity of formaldehyde resulted in an inhibition of both nuclear and cytoplasmic syntheses, and created the normal bacteriostatic condition that has been observed before with sublethal concentrations of formaldehyde.

Anti-Bacterial Agents↗
PubMed1962

Studies on immunity in anthrax. IX. Effect of variations in cultural conditions on elaboration of protective antigen by strains of Bacillus anthracis.

Wright, George G. (Fort Detrick, Frederick, Md.), Milton Puziss, and W. Brook Neely. Studies on immunity in anthrax. IX. Effect of variation in cultural conditions on elaboration of protective antigen by strains of Bacillus anthracis. J. Bacteriol. 83:515-522. 1962.-Nonproteolytic and nonencapsulated avirulent mutants were isolated from six virulent strains of Bacillus anthracis and tested for elaboration of protective antigen in the chemically defined medium developed previously (599 medium). Initially the strains grew rather slowly in 599 medium; serial transfer in the medium increased the rate of growth but reduced the elaboration of antigen. Two of the strains tended to revert to the encapsulated form during serial passage in 599 medium. Modifications in the medium and cultural conditions were studied in an attempt to obtain improved growth; the most significant alterations were the addition of adenosine and l-alanine, reduction in the concentration of ferrous sulfate, and growth under anaerobic conditions with agitation. Study of amino acid utilization in the medium revealed that the concentrations of certain amino acids could be reduced. These modifications, together with substitution of more productive strains, resulted in an approximately fivefold increase in elaboration of protective antigen, and greatly simplified production of the antigen on a practical scale. Omission of bicarbonate from the medium had no significant influence on utilization of amino acids.

Alanine↗