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Biomedical subjects

W B Stavinoha

Publications and source records attributed to W B Stavinoha.

At least 55 records · Page 3Linked to original sources
PubMed1975

Dichlorvos and the cholinergic system: effects on cholinesterase and acetylcholine and choline contents of rat tissues.

The inhibition of cholinesterase by 50 mg/kg/5 ml, p.o., dichlorvos and subsequent recovery of enzyme activity was uniform in all brain regions studied. This uniformity was not observed in liver, erythrocytes and plasma. Acetylcholine levels were elevated in brain areas from 48 to 171% at 15 minutes after treatment. However, a biphasic effect was seen on choline metabolism in the brain. The cortex was found to be more cholinergic than the striatum in terms of per cent increase in acetylcholine and choline.

Acetylcholine↗
PubMed1974

Choline content of rat brain.

The free choline concentration in the rat brain was found to be 26.3 nanomoles per gram of brain tissue. This value was obtained through use of 6-second microwave irradiation for killing animals and inactivating enzymes, followed by a pyrolysis-gas chromatographic assay procedure. The identities of compounds measured from brain samples were verified by mass spectrometry.

Acetylcholine↗
PubMedDate not supplied

Use of microwaves for rapid fixation of tissues in vivo.

Accurate knowledge of the concentration in the central nervous system of neurochemicals undergoing rapid enzymatic destruction or synthesis is sparse because of the difficulty in stopping the rapid reactions while causing only minimal adverse changes in the neurochemistry and structure. Microwave heating can be effectively used to rapidly stop enzyme activity in the central nervous system with minimal adverse changes. This rapid inactivation of the enzymes increases the validity of the sample that is taken for analysis of the concentration of the enzyme's substrate.

Animals↗