Biomedical subjects
Publications and source records attributed to W Bartnikowa.
DNA polymerase (reverse transcriptase) from Gross virus was characterized with regard to template specificity. Changes in pH in the reaction medium in vitro exert a varying influence on this enzyme's activity depending on the template used.
Perinatally thymectomized AKR mice inoculated i.v. with acute lymphoblastic T-cell leukemia (TAL) demonstrated, as compared to controls, an accelerated passage of injected cells through the lungs. Later in disease they also exhibited an especially intense infiltration of liver and spleen by leukemic blasts proliferating at a slow rate the big majority of cells being in the G0 + G1-phase. The thymus controls the proliferation and the traffic of leukemic cells in the recipient.
In transplantable and in spontaneous lymphoblastic mouse leukemia blood pH, PCO2, TCO2 and BB were examined. Spontaneous Gross-leukemia in AKR mice was found to develop in its final phase respiratory acidosis. Transplantable (TAL) leukemia of AKR mice presents from the onset a tendency toward respiratory acidosis. "L-1210"-leukemia, on the contrary, was shown to alcalise the recipients during the first 8 days after inoculation, later on it can bring about an acidosis. The pH-deviations in "L-1210"-leukemia display a respiratory character in syngeneic DBA/2J recipients, whereas in semiallogeneic recipients the observed changes are metabolic or mixt in nature. This finding strongly argues for the importance of histocompatibility. Intranodally inoculated animals distinctly differ in their parameters in comparison to intravenously and intraperitoneally recipients, hence, an important role of the tissular-milieu which is put first in contact with the leukemic factor must be concluded.
In mice vaccinated with two forms of lymphoblastic leukaemia and alkalized with intravenous administration of sodium bicarbonate, the survival rate, the extent of leukaemic infiltration and the proliferative capacity of cells in the bone-marrow, thymus, spleen, lymphnodes, liver and lungs were investigated. The survival rate in the TAL leukaemia of the AKR stem producing an endogenous acidosis could be significantly prolonged in a statistical way by alkalization. Yet an accelerated expiring rate could be observed after exogenous alkalization in L-1210 leukaemia of the DBA/2J stem producing an endogenous alkalosis. By means of cytological and impulse-cytophotometrical investigations the exogenous alkalization of both forms of leukaemia could be proved to have a direct bearing on the proliferative kinetics. In TAL leukaemia the leukaemic proliferation was inhibited by the exogenously involved correction of the acid-base balance; in the L-1210 leukaemia, however, the pH disturbances were enhanced, thus accelerating the leukaemic proliferation. Consequently, the disturbances of the acid base balance seem to be an essential cofactor in the leukaemia genesis. The exogenous direction of the acid-base balance may be important as a means of treating leukaemia.
In a 53-year-old female patient, a tumor localized in the nasopharyngeal cavity together with hematologic features of acute paramyeloblastic leukemia were observed. More than 70% blast cells contained giant intracytoplasmic inclusions which have been found to be strongly pyroninophilic. The electron-microscopic study revealed big agglomerations of ribosomal RNA inside of pseudofibrillar structures of the cytoplasma. The possibility of a new nosological entity should be envisaged.
Revertase present in the myeloblasts of chickens infected with avian myeloblastosis virus failed to synthetise DNA in vitro on the endogenous viral template. Enzyme inhibitors were not blocking this synthesis. It was rather impaired by incompletness of viral RNA in immature virus particles.