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Biomedical subjects

W Beckmann

Publications and source records attributed to W Beckmann.

10 recordsLinked to original sources
PubMed1993

The promoter of the gene encoding 3',5'-cyclic adenosine monophosphate (cAMP) response element binding protein contains cAMP response elements: evidence for positive autoregulation of gene transcription.

The transcriptional transactivational activities of the phosphoprotein cAMP-response element-binding protein (CREB) are activated by the cAMP-dependent protein kinase A signaling pathway. Dimers of CREB bind to the palindromic DNA element 5'-TGACGTCA-3' (or similar motifs) called cAMP-responsive enhancers (CREs) found in the control regions of many genes, and activate transcription in response to phosphorylation of CREB by protein kinase A. Earlier we reported on the cyclical expression of the CREB gene in the Sertoli cells of the rat testis that occurred concomitant with the FSH-induced rise in cellular cAMP levels and suggested that transcription of the CREB gene may be autoregulated by cAMP-dependent transcriptional proteins. We now report the structure of the 5'-flanking sequence of the human CREB gene containing promoter activity. The promoter has a high content of guanosines and cytosines and lacks canonical TATA and CCAAT boxes typically found in the promoters of genes in eukaryotes. Notably, the promoter contains three CREs and transcriptional activities of a promoter-luciferase reporter plasmid transfected to placental JEG-3 cells are increased 3- to 5-fold over basal activities in response to either cAMP or 12-O-tetradecanoyl phorbol-14-acetate, and give 6- to 7-fold responses when both agents are added. The CREs bind recombinant CREB and endogenous CREB or CREB-like proteins contained in placental JEG-3 cells and also confer cAMP-inducible transcriptional activation to a heterologous minimal promoter. Our studies suggest that the expression of the CREB gene is positively autoregulated in trans.

Amino Acid Sequence↗
PubMed1989

[Computer-assisted control of a public health inventory].

To cope with its tasks more efficiently, the Public Health Office of the "Märkische Kreis" in 1985 installed an information system on the basis of electronic data processing, the so-called "hygiene inventory". Initially, the introduction of this system into the local Public Health Office is described. The structure and organization of the programme and its performance are then discussed and exemplified by the control of drinking water supply plants. The disadvantages of computer use are by no means overlooked. The latter include the necessity to initially put in a considerable number of data and to constantly store new results, initial acceptance problems and the poor autonomy of the system. The most important advantages of computer-aided processing are optimum evaluation possibilities, centralised scheduling, automated production of letters, efficient drafting of the annual health report and the possibility of exchanging data media.

Electronic Data Processing↗
PubMed1988

[Von Recklinghausen's neurofibromatosis with rectal involvement].

Per-rectum bleeding was noted in a 71-year-old woman known to have neurofibromatosis. At rectoscopy, a malignant looking tumour was seen, beginning at 8 cm from the anal sphincter. It protruded into the lumen, disintegrating at its centre and oozing blood. Histological examination of the biopsy revealed a neurofibroma, without evidence of malignancy. It was possible to perform a deep rectum resection with continence-preserving end-to-end anastomosis and complete removal of the neurofibroma.

Aged↗
PubMed1987

[Intestinal hemorrhage due to multiple phlebectasias of the small intestine].

A 37-year-old man had recurrent intestinal bleeding and resulting chronic anemia from multiple phlebectasias of the small intestine. Contrast medium studies and endoscopy of the intestine were negative. Abdominal angiography, however, demonstrated phlebectasias in the region supplied by the jejunal arteries. At operation and on examination of a resected portion of the jejunum, these multiple phlebectasias were demonstrated. Resection of the worst affected portion of the jejunum with end-to-end anastomosis was without complications postoperatively, the benzidine test on faeces was negative, and the blood-hemoglobin level gradually rose. Since radiological examination with contrast media and endoscopy are often negative in bleedings from vascular malformations of the intestine, abdominal angiography should be performed in case of intestinal bleedings not diagnosed by other methods.

Adult↗
PubMed1981

Enrichment and isolation of naphthalenesulfonic Acid-utilizing pseudomonads.

Naphthalenesulfonate-degrading bacteria were obtained by continuous enrichment from a naphthalene-degrading population from sewage. In addition to naphthalene, Pseudomonas sp. A3 can utilize 2-naphthalenesulfonate (2NS) and Pseudomonas sp. C22 can utilize both 1-naphthalenesulfonate (1NS) and 2NS as sole carbon sources. In a mixture of 1NS and 2NS, the former substrate is utilized by strain C22 only after complete consumption of 2NS. During exponential growth, approximately 10% of the organic carbon of naphthalenesulfonates is temporarily excreted. These unidentified metabolites can readily be used by other bacteria, which, by supplying strain C22 with vitamins, allow optimal growth in stable mixed cultures. The degradative capability of Pseudomonas sp. A3 for 2NS was irreversibly lost under nonselective growth conditions and could be transferred from the wild type to a distinguishable cured strain of the wild type.

Journal Article↗
PubMed1981

Catabolism of Naphthalenesulfonic Acids by Pseudomonas sp. A3 and Pseudomonas sp. C22.

Naphthalene and two naphthalenesulfonic acids were degraded by Pseudomonas sp. A3 and Pseudomonas sp. C22 by the same enzymes. Gentisate is a major metabolite. Catabolic activities for naphthalene, 1-naphthalenesulfonic acid, and 2-naphthalenesulfonic acid are induced by growth with naphthalene, 1-naphthalenesulfonic acid, 2-naphthalenesulfonic acid, methylnaphthalene, or salicylate. Gentisate is also an inducer in strain A3. Inhibition kinetics show that naphthalene and substituted naphthalenes are hydroxylated by the same naphthalene dioxygenase. Substrates with nondissociable substituents such as CH(3), OCH(3), Cl, or NO(2) are hydroxylated in the 7,8-position, and 4-substituted salicylates are accumulated. If CO(2)H, CH(2)CO(2)H, or SO(3)H are substituents, hydroxylation occurs with high regioselectivity in the 1,2-position. Thus, 1,2-dihydroxy-1,2-dihydronaphthalene-2-carboxylic acids are formed quantitatively from the corresponding naphthalenecarboxylic acids. Utilization of naphthalenesulfonic acids proceeds by the same regioselective 1,2-dioxygenation which labilizes the C-SO(3) bond and eliminates sulfite.

Journal Article↗
PubMed1976

[On the mechanism of the biological persistence of halogenated and sulfonated aromatic hydrocarbons (author's transl)].

Aromatic compounds with unphysiological substituents like halogen or SO2H-groups are mainly degraded by cometabolism. Investigations with model compounds show, that the negative inductive effect (-I-effect) of the halogen substituents impede the electrophilic attack of the oxygenases, particularly the pyrocatechases. Although the "affinity" of the enzyme for the substrate increases with the number of halogen substituents, the rate of ring cleavage decreases by the presence of halogen substituents. Benzoate oxygenation shows that in certain positions of substitution the -I-effect can be weakened by the + M-effect of the halogen. As soon as aromaticity is lost by the action of dioxygenases, halide can be eliminated with greater ease and total mineralization is possible. When naphthalene-2-sulfonic acid is degraded by a naphthalene utilizing Pseudomonas strain the sulfonic acid group appears to be eliminated oxygenolytically and the carbon skeleton is channelled into the naphthalene pathway.

Alcaligenes↗