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W Biczysko

Publications and source records attributed to W Biczysko.

At least 37 records · Page 2Linked to original sources
PubMed1998

Is vacuolization of podocytes and glomerular endothelial cells of prognostic value with respect to FSGS?

Focal segmental glomerulosclerosis (FSGS) poses a major problem both from the clinical and pathomorphological viewpoint. The diagnosis of FSGS in its early stage is vital mainly because of rapidly developing therapeutic modalities. In the literature various changes are discussed which may be of prognostic value (may predict the development of FSGS). One the these changes in vacuolization, mainly of podocytes and less frequently of endothelial cells. The purpose of the present study was to analyse biopsy specimens to find out to what extent vacuolization of podocytes and endothelial cells is associated with FSGS. We compared vacuolization in minimal change disease (MCD), mesangial glomerulonephritis (GNMES) and FSGS. A similar analysis was made also with respect to those cases of MCD and GNMES, in which electron microscopy suggested an early stage of FSGS. In each group electron micrographs obtained from 15 children were analysed. Electron micrographs (12-15 on average) were obtained most frequently from 3 glomeruli. Each case electron micrographs contained 90-100 podocytes. Based upon the same electron micrographs we counted capillary lumina and defined the percentage of those which contained vacuolized endothelia (we counted the capillary lumina, and not the cells, because it is most frequently impossible to identify the border of vacuolized endothelial cells). The number of capillary cross-sections was 60 on average. The results of the analysis were compared with the clinical data. This comparison did not confirm the hypothesis of other investigators that vacuolization is of a prognostic value. Additionally we evaluated the character of vacuoles. Within podocytes the vacuoles were varying in shape. Surrounded by a single membranous layer most frequently they contained material corresponding to proteins or proteoglycans, rarely to lipids. Sometimes the vacuoles were autophagosomal, occasionally they consisted of the dilated rough endoplasmic reticulum. Vacuole-like changes within the capillary lumina were related to the swelling of endothelial cytoplasm or mesangial processes. The reasons for a discrepancy between our results and those reported by other investigators necessitate further studies.

Child↗
PubMed1998

[The value of DNA flow cytometry in evaluation of proliferating activity of neoplasm cells in patients with laryngeal cancer].

A study of DNA ploidy was carried out including 84 cases of laryngeal squamous cell carcinoma. Material was fixed during 30 min in 3% paraformaldehyde and embedded in low-melting-point paraffin. The histologic studies were done for diagnosis and general view. Results were as follows. The highest percentage of cancers with DNA diploidy was found in well- and medium-differentiated tumors. Among well-differentiated carcinomas there were 18% diploid versus 5% polyploid tumors. In the medium-differentiated cancers there were 23.5% diploid, 6% polyploid, and 6% hyperdiploid tumors. The highest percentage of DNA hyperdiploidy and poliploido-aneuploidy was found in low-differentiated cancers. Additionally, tumor proliferative activity was evaluated in relation to the percentage of cells in phase S of cellular cycle. Special attention was paid to tumors which revealed the phenomenon of DNA poliploidy-aneuploidy beside DNA diploidy.

Carcinoma, Squamous Cell↗
PubMed1997

Alteration of p53 gene structure and function in laryngeal squamous cell cancer.

The p53 gene is known as an anti-oncogene that manifests its function by controlling the cell cycle and is responsible for apoptosis of cells with unrepaired DNA. An accelerated p53 protein synthesis is the first response of a cell following DNA damage. However, mutations of the p53 gene can disturb protein synthesis or may be responsible for synthesis of a changed protein unable to control the cell cycle. Laryngeal tissue specimens from 120 patients were tested by immunohistopathological staining to detect mutated wild-type p53 protein. It was found that p53-positive specimens correlated with TNM staging and histopathological grading. Another indication of entering the cell cycle and undertaking an active proliferation by laryngeal cells was shown by detection of proliferating cell nuclear antigen (PCNA) and Ki67 nuclear antigen, which appeared in proliferating cells (late G1, S-G2 and M phase), but was absent in resting cells. Scoring of the staining for p53 protein, PCNA and Ki67 correlated with each other. DNA from 40 specimens was then isolated, amplified by polymerase chain reaction and analysed by single-strand conformation polymorphism and DNA sequencing for mutation in the p53 gene. Fifteen DNA samples were found to be positive, while mutations were detected in exons 5-8 in 13 samples. The majority of mutations were found in tissue specimens from T3 and T4 tumors. A possible explanation is almost half was attributable to genotoxic effects of tobacco smoking. Changes in the p53 gene and its products may also reflect early changes in laryngeal carcinogenesis and be of prognostic value.

Adult↗
PubMed1997

Clinical and morphological aspects of Buerger's disease.

BACKGROUND: Thirty-five patients with TAO and 35 with ASO have been studied. To determine differences between these two arterial diseases, we used clinical criteria, arteriographic and morphological methods. The peripheral (below the bifurcation of the popliteal artery) angiographic changes were found in TAO with 94% sensitivity, 94% specificity and with 96% of positive predictive value. Other clinical diagnostic criteria for differential diagnosis had a lower value (they had high sensitivity and low specificity or low sensitivity and high specificity). RESULTS: The histologic studies confirmed the clinical diagnoses of Buerger's disease in 92,9% cases. The pathologic findings in TAO and ASO were different. CONCLUSIONS: Our investigations proved that the characteristics of Buerger's disease include the development of changes not only in small and medium size arteries or veins, but also in microcirculation.

Adult↗
PubMed1997

Morphology of brain stem lesion and bera findings after 60Co irradiation.

Ionizing rays, utilized in radiological diagnostics and oncological therapy affect the central nervous system and may injure auditory pathways and cause hearing disturbances which vary in intensity. On the basis of a stereotactic atlas of the brain of a guinea pig, the trapezoid and geniculate bodies were identified in the skull X-ray pictures. The irradiated region was found to have 10 x 6 x 5 mm in dimensions and to be situated at the depth of 11 mm away from the animals occiput. After introductory recording of the potentials obtained from the brain stems (BERA), the stems of 60 guinea pigs were irradiated in the groups with the doses of 5, 10, 20 Gy. The hearing potentials from the brain stems were recorded on the 1st, 4th, 10th, 21st and 84th day after irradiation. The findings of measurements of hearing potentials were compared with the morphologic picture of the brain stems in the examined animals.

Animals↗
PubMed1997

The Kartagener syndrome--an electron microscopic studies of biopsied mucosa.

We studied 43 tissue samples taken from patients (16 male and 27 women) with clinical diagnosis of Kartagener's syndrome. The specimens were fixed and prepared for transmission electron microscopy. After preliminary light microscopic examination 23 cases were qualified for ultrastructural studies. We found abnormal number of cilia on the bronchial mucosa cells. The ciliary structure was abnormal. In several cases we observed lack of inner and/or outer dynein arms, abnormal location of central microtubules, and abnormal number of microtubular doublets. Other findings were as follows: replacement of ciliated cells by goblet ones, mucosal edema and inflammatory infiltrate.

Adolescent↗
PubMed1996

[Diagnostic and prognostic value of p53 oncogene and the selected neoplastic markers (Ki67, PCNA, DNA ploidy) of the ultrastructure in patients with laryngeal cancer].

A comparison was performed of staining intensity of immunohistochemical proliferating antigens (p53, PCNA, Ki67), DNA flow cytometry and ultrastructure of the carcinoma cells in 120 cases of laryngeal cancer. Clinically very advanced tumors were in majority (T3 - 43%, T4 - 18%). A 5 graded scale was adapted to evaluate the level of immunohistochemical staining of the carcinoma cell nuclei. A positive staining was obtained in 70% for p53, 57% for Ki67 and in 80(2/3) for PCNA. 62% of the cases were DNA diploid and 38% DNA aneuploid. The DNA diploid carcinomas were accompanied by the enlargement of the cell nuclei, preserving of the nuclei's wide margins of heterochromatine, enlargement of the nuclear area and increase of the number of nuclei. In the aneuploid-polyploid cancer the nuclei had a substantial polymorphism with large cleaved nuclei and with significant variation in size, and with nuclear envelope. A frequent finding was euchromatization of chromatine. Dense chromatine appeared in the form of small clumps spread over the whole area of these irregular nuclei. Enlargement and activation of nucleoli occurred. There was a positive correlation (Chi-square) between T- and N-stage and immunohistochemical staining. There was also a positive correlation in staining intensity between p53, Ki67 and PCNA. There is also strong correlation between these markers of proliferative activity and the degree of aggressiveness of the tumour.

Adult↗
PubMed1995

The entrance of surface active material to pulmonary capillaries in the lungs of newborn rats.

In newborn rats we investigated the entrance of surfactant to the pulmonary capillaries a few minutes after the birth. Time is crucial for the process which can be called "drying" of the lung, when a large amount of intraalveolar fluid enters the pulmonary circulation. Ultrastructural studies have revealed bulk transport and endocytosis of surfactant via alveolar septa to the blood vessels, but it is still questionable whether the entrance of surfactant to pulmonary capillaries is related to its clearance or to "drying" of lung's alveoli.

Animals↗
PubMed1995

[Interferon alpha in the treatment of chronic hepatitis B in children].

Nine children suffering from chronic hepatitis due to hepatitis B virus (HBV) infection were treated with recombinant IFN-alpha-2C for 16 weeks. Replication of HBV was inhibited in 5 cases while a decreased inflammatory process in the liver was attained in 3. The treatment was very well tolerated in all of the studied children. A significant increase of ALT activity in the fourth week of treatment seems to be a positive prognostic factor.

Adolescent↗
PubMed1994

Neuroendocrine component of different types of the pulmonary carcinoma.

One hundred and seventy lung tumors were examined: 155 squamous cell carcinoma, 12 adenocarcinomas and 3 mixed adenosquamous carcinoma. In electron microscopic examination the neuroendocrine cells were found: in 31 cases of squamous carcinoma, in 9 cases of adenocarcinoma, and 1 case of mixed adenosquamous carcinoma.

Adenocarcinoma↗
PubMed1994

[Measurements of DNA-ploidies in patients with carcinoma of the larynx].

In recent years there has been a lot of research done on using DNA-cytometry in the evaluation of larynx cancer. Everyone unanimously emphasizes that aneuploidal and polyploidal neoplasms require special therapeutical treatment. The definition of proliferating activity of neoplasm is of utmost importance. Other than diploidal amount of DNA is characteristic for cells fast dividing and incorrectly dividing. For the evaluation of own material, 20 cases of larynx cancer were collected and underwent a routine morphological and electron microscope evaluation, also flow-cytometry of DNA on Coulter apparatus was carried out. Generally, it can be said that cytometry of DNA in comparison with morphological examination has a secondary function but is nevertheless and important test. It allows the doctor to be more objective in evaluating the population of dividing cells.

DNA↗
PubMed1994

Retardation of lung development after maternal cigarette smoking and low protein diet in animal model.

This study has been aimed at showing the influence of tobacco smoke, together with low protein diet on the lungs of newborn rats. The studied animals were Wistar rats. They were exposed to the tobacco smoke for 11 weeks (6 weeks before mating, 12 days during mating, and 21 days of pregnancy). Morphological analysis and interpretation of 48 one-day old rats was based on observation under the light and electron microscope. The study showed atelectasis of the lungs: the lung tissue was insufficiently extended, the alveoli were geometrically irregular and the alveolar septa were thick. Many extravasated erythrocytes and edema (latent as well as overt) were also present. The II type pneumocytes and their surfactant were poorly developed. Tobacco smoke caused the serious pathological effects and together with poor protein diet all changes worsened. For comparison, the lungs of control group were studied (lack of exposure to tobacco smoke); they were well aerated and their structure well-developed.

Animals↗
PubMed1993

Application of electron microscopy and immunocytochemistry in lung cancer diagnosis.

Using bronchofiberoscopy the biopsies of '50 small cell carcinomas of the lung (SCLC) were diagnosed. The material was studied by immunocytochemical methods for cytokeratins and in routine transmission electron microscopy. The presence of bundles of cytokeratins was specific for squamous epithelium, in all histological subtypes of small cell carcinomas. The number and distribution of cytokeratins were described. In whole material we found the neuroendocrine cells in which polymorphic neuroendocrine granules were present.

Carcinoma, Small Cell↗
PubMed1993

[Parakeratosis of larynx as a base of cancerogenesis].

Keratosis, parakeratosis and hyperkeratosis of the larynx and hyperkeratotic papilloma with the inflammation should be treated as clinical precancerous state. Parakeratosis and cellular dysplasia are histological features of cancerogenesis. The patient with extensive verrucous keratosis of the larynx (described in the article) has been observed for 7 years. Eventually, the developed verrucous carcinoma of the larynx.

Carcinoma, Verrucous↗
PubMed1987

Medulloblastoma: histological evaluation and prognosis. A clinical, immunohistochemical and electron microscopic study.

Eighty-four cases of medulloblastoma were examined immunohistochemically and 12 by electron microscopy to assess differentiation in these tumors. Based upon the largest series of medulloblastoma studied to date, we demonstrated glial fibrillary acidic protein (GFAP) positivity, in 25% (21/84) of these tumors showing glial differentiation. GFAP-positive cells were seen more frequently in the desmoplastic variant of medulloblastoma (7/10). Under electron microscopy, the major part of the 12 tumors studied appeared primitive and undifferentiated. In 7 cases, groups of cells were found with primitive neuronal and/or glial features. GFAP positivity was confirmed at light microscopy level in all cases where cells showed glial differentiation in the form of glial-like filaments in cytoplasma. However, a follow-up questionnaire study of those patients who had received only surgical treatment revealed no difference in mean survival time between GFAP-positive and GFAP-negative medulloblastoma.

Adolescent↗