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Biomedical subjects

W Breipohl

Publications and source records attributed to W Breipohl.

At least 55 records · Page 3Linked to original sources

Fine structure of the vomeronasal neuroepithelium of bats: a comparative study.

The vomeronasal neuroepithelium of the vampire bat, Desmodus rotundus, and the frugivorous bats, Carollia perspicillata and Artibeus jamaicensis, was examined at the light-microscopic and ultrastructural levels. The neuroepithelium of these species is basically similar in morphology to that of other mammals. However, hitherto unknown differences were noted in regard to the configuration of the apical poles of cells, in the occurrence of solitary cilia, dislocated sensory cells, brush cells, cell contacts and the occurrence and fine structure of basal cells. The morphology of the vomeronasal neuroepithelium of all three species is discussed from a comparative point of view in regard to chemoreception.

Animals↗

Cell migration from the chick olfactory placode: a light and electron microscopic study.

The differentiation of the olfactory placode in the chick has been studied using light and electron microscopy. Special attention was paid to the appearance of neuronal cells within the placodal ectodermal thickening, the migration of cells out of this tissue and the appearance of the first fila olfactoria in the differentiating olfactory mucosa. Between the third and fifth day of incubation a large number of cells is observed leaving the base of the invaginating olfactory placode, often in contact with thin axon bundles. These cells are characterized by a well-developed Golgi apparatus, a considerable number of mitochondria and dense-core vesicles. The morphology of these migrating cells resembles that of cells observed near the basement membrane within the developing olfactory epithelium and is clearly different from the mesenchymal cells which are filled with polyribosomes. At the sixth day of incubation thick axon bundles can be observed within the epithelium and the underlying lamina propria. The possible fate of the migrated epitheloid cells is discussed.

Animals↗

Spatial pattern of sensory cell terminals in the olfactory sac of the tiger salamander. I. A scanning electron microscope study.

The olfactory sac of adult tiger salamanders (Ambystoma tigrinum) was assessed by scanning electron microscopy and the hitherto unknown variety of the olfactory sac cells has been documented. Regional differences in respect to the composition of the apical epithelial surface border in the olfactory sac have been recorded. Transitions between the olfactory epithelium proper and epithelia with different surface structures have been described. It has been shown that both the roof and the floor of the olfactory sac are mainly covered by sensory epithelium with a surface of uniform appearance. However, the sensory area of the floor is interspaced with non-olfactory epithelial bands. Variations were also noted in regard to secretory cells, cells with conical profiles, microplicae arrangements and populations of microvilli and kinocilia. These differences have been discussed in relation to electrophysiological data which suggest a regional patterning in the response to odours.

Ambystoma↗

Intraepithelial blood vessels in the vomeronasal neuroepithelium of the rat. A light and electron microscopic study.

Epithelial-vascular relationships are established during the development of the vomeronasal neuroepithelium of the rat. Special attention is given to the fine structure of the endothelial wall of intra-epithelial vessels, to ultrastructural aspects of the neuronal-vascular relationships, and to the appearance of inclusion bodies in the neuronal cells adjacent to these vessels. The neuronal perikarya surrounding the blood vessels are filled with highly developed smooth endoplasmic reticulum. Possible functional implications of the vascularization of the neuroepithelium of the vomeronasal organ in mediating olfacto-endocrine relationship are discussed. It is suggested that the intra-epithelial blood vessels are at least supportive and nutritive in nature, while their implication in an olfacto-endocrine connection remains obscure.

Animals↗

Freeze-etching studies on the ciliary necklace in the rat and chick.

The existence of a ciliary necklace in kinocilia, atypical cilia and mature and differentiating sensory cilia is described in rat and chicken. In addition to parallel horizontally oriented rows of the ciliary necklace, irregular forms of intramembranous particle (IMP) aggregations are also described. The function of the ciliary necklace is discussed, special attention being given to the appearance of circular rows of IMP prior to the outgrowth of olfactory cilia and to the existence of dynein arms in these. Evidence is provided for the motility of olfactory cilia in the chick.

Animals↗

[Chemical preserved human trachea as prosthesis in covering tracheal defects--first experiences (author's transl)].

Basing upon animal experiments at the first time in two cases parts of a chemically conserved trachea were used for closing tracheal defects in reconstructive procedures. After layering a silastic foil on mucosal surface of the conservated tracheal piece in a first step this prepared transplant is implanted beneath the tracheal defect subcutaneously. Hitherto after 2 vs 3 months in a second step the paratracheal preimplants, now vascularized, were turned about the defect like a pedicle flap. Both transplants healed in the tracheal defect without disturbances. The new inner surface of the trachea now after an observation up to 6 months has remained plain without any granulations.

Follow-Up Studies↗

Fine structure of the receptor-free epithelium in the vomeronasal organ of the rat.

The topography and distribution of the epithelia of the vomeronasal organ were investigated in the adult male rat. Special attention was given to the light and transmission electron microscopic structure of the area that is known to be free of receptor cells. In contrast to several brief descriptions in which many cell types have been reported, only one cell type is presently described in different functional phases. In the rat, this epithelium does not show the typical features of a respiratory or, in more general terms, non-sensory epithelium. The authors propose to call this epithelium as "receptor-free" rather than respiratory, non-sensory or ciliated. The importance of the vomeronasal organ in the maintenance of olfacto-endocrine mechanisms and reproductive behavior is discussed.

Animals↗

Ultrastructural investigation on the cell membranes of the vomeronasal organ in the rat: a freeze-etching study.

The free surfaces and cell contacts in the epithelia of the vomeronasal organ of the rat were investigated by freeze-etching. The microvilli of receptor cells show a lower density of intramembranous particles (IMP) than the microvilli in the receptor-free epithelium. The ratio between the IMP on P- and E-face is approximately 11 : 1 in the receptor terminals, and 3.5 : 1 in the cilia and microvilli of the receptor-free epithelium. Although atypical in length and only poorly equipped with rootlet fibers, the cilia of the receptor-free epithelium are furnished with typical ciliary necklace structures of up to 10 rows of membrane particles. Differences in the density of IMP on the P-faces of different cilia are probably due to continual ciliogenesis and also due to the different age of cilia in the receptor-free epithelium. Zonulae occludentes show different configurations in the neuroepithelium and in the receptor-free epithelium. In the former, they show a tendency to cross-link and form facet-like patterns, reflecting a constant morphology and relative stability for this apical region. In the receptor-free epithelium the junctional rows of zonulae occludentes display only loosely interconnected networks and a tendency to orient parallel to each other and to the free surface. In addition to zonulae occludentes, typical square aggregations of IMP are observed in the receptor-free epithelium. They are not exclusively restricted to the zone of intensive cell contacts by means of fine interdigitating cell processes, and their function has yet to be identified experimentally.

Animals↗

[Two rare congenital malformations of the female urethra].

Two cases of incontinent females due to malformations of the dorsal wall of the urethra are presented and surgical corrections are discribed. The causal factors for the development of the malformations are explained by irregularities of the Wolffian ducts' regression during the transition period between in gonadal development between the 3rd and 5th week of gestation. The new medical technical term "indifference-syndrome" is proposed, to distuingish these malformations of the female urethra from true hypospadia.

Child, Preschool↗

[Scanning electron microscopy studies on epithel surface of the plica vocalis in male rats (author's transl)].

The epithelial surface of the Plica vocalis in adult male rat is studied by scanning electron microscopy. It's structure is compared with that of the plica and of the recessus ventricularis. The transition of the epithelium in the plica vocalis to the epithelium of the joining parts of the larynx is documented. Special attention is paid to the occurrence of microridges at the apical poles of epithelial cells in the larynx. Up to now microridge cells have been said to be characteristic for the plica vocalis.

Animals↗

Scanning electron microscopic investigations of olfactory epithelium in the chick embryo.

This paper describes maturation of the surface of the olfactory epithelium of the superior nasal concha of chick embryos from the seventh day of incubation till hatching. From the earliest stages investigated onward, the apical surface of the olfactory epithelium is characterized by variability in the number and configuration of surface protrusions. The growth of olfactory cilia is described and the significance of regional differences in their density is discussed in relation to regional variations in the sensitivity of the epithelium to odorants. The maturation of the respiratory epithelium covering the inferior nasal concha is also described.

Animals↗

[Scanning electron microscopy studies concerning the epiglottis epithelia of rats (author's transl)].

The epithelial surface of the epiglottis in adult male and female albino rats is studied by scanning electron microscopy. Prominent differences exist between the surface morphology as observed in the males and that observed in the females sacrificed in the estrus phase of the ovarial cycle. The differences are most pronounced in respect to the amount of secretory vesicles, microvilli- and microridge-cells and in respect to the observable number of desquamating cells. The existence and the importance of the microridge-cells are discussed. Special attention is also paid to the results indicating that at least some parts of the extrapulmonary airway epithelia act as possible target organs for sex hormones. Finally the importance of the observed differences is discussed for the correct interpretation of pathomorphological alterations in the extrapulmonary airway epithelia that have been observed under experimental conditions and during infections.

Animals↗

Microridge cells in the larynx of the male white rat. Investigations by reflection scanning electron microscopy.

The laryngeal epithelium of male white rats is studied by reflection scanning electron microscopy (SEM). In addition to ciliated cells, microvilli cells, brush cells and goblet cells that are characteristic for normal respiratory epithelium the microridge or labyrinth cell can be seen in particular regions of the larynx. The apical surface of a typical labyrinth cell is characterized by a system of narrow standing microridges of about 0.05--0.15 micronm in diameter and interconnecting microridges with a diameter of about 0.01 micronm. The microridge system of a labyrinth cell originates from the fusion densely standing microvilli. Between microridge cells and microvilli cells all transition forms can be observed. The preferable localiziation of the microridge cell in the larynx and its possible function is discussed.

Animals↗

Morphology and distribution of supraependymal cells in the third ventricle of the albino rat.

The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and stellate or spider-like cells, transitional forms between these types can be seen. Their perikarya and processes can either remain at some distance from the ependyma or be in close contact with it. The processes may protrude between the ependymal cells or show surface differentiations that resemble the thin cytoplasmic folds of the mesenchymal wandering cells. Considering this and the variations in the number of cells, for example during the ovarian cycle, the SEC can be interpreted as mesenchymal cells, probably related to microglial cells of the subependymal layer. It is suggested that the SEC have a phagocytotic function and may be involved in the normal renewal of the ependyma. A definitive explanation for the restriction of the SEC to the hypophyseotropic area as well as the elucidation of their function remain to be found.

Animals↗

Surface morphology in the ofactory epithelium of normal male and female rhesus monkeys.

Olfactory epithelium of male and female rhesus monkeys has been studied by transmission electron microscopy. It is shown that the epithelial surface differs greatly between male and female monkeys. Differences are most prominent between male and preovulatory females. During the ovulatory phase of the ovarian cycle remarkable histological alterations occur especially in the sustentacular cells. These alterations are correlated with physiological and behavioral phenomena demonstrating a possible structural basis for the changes in olfactory threshold in relation to sex hormones.

Animals↗

[Epithelial surface of trachea in adult male albino rats (author's transl)].

The epithelial surface of trachea in adult male albino rats is studied by reflection scanning electron microscopy (REM). Surface morphology of the tracheal epithelium shows extensive individual and regional variability. Identical surface morphology may result by different causal factors. Regional morphology is presented by a mapping of the epithelial surface. Special attention is given to the structure of the cilia. Furthermore the morphology and distribution of possible receptor cell terminals is shown. The validity of reflection scanning electron microscopic studies for identifying the patho-histogenetic action of air pollutants is discussed.

Animals↗