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Biomedical subjects

W C Hayes

Publications and source records attributed to W C Hayes.

At least 145 records · Page 8Linked to original sources

Stress analysis of compression plate fixation and its effects on long bone remodeling.

A three-dimensional finite element model is generated for an intact plexiglass tube with an attached six-hole stainless steel compression plate. The results for a wide range of loads, including cyclic external loads and static tensile preloads in the plate and screws, are examined as specifically related to plate-induced osteopenia. The model demonstrates that disuse osteopenia, resulting from a reduction in magnitude of cyclic axial stress, should be limited to the central region between the inner screws. Also, the addition of a static preload negates any reduced axial stress levels in this region, thus raising questions on the relative importance of static and cyclic stresses for the internal remodeling of bone.

Animals↗

Tensile strength of bovine trabecular bone.

Data on the tensile and compressive properties of trabecular bone are needed to define input parameters and failure criteria for modeling total joint replacements. To help resolve differences in reports comparing tensile and compressive properties of trabecular bone, we have developed new methods, based on porous foam technology, for tensile testing of fresh/frozen trabecular bone specimens. Using bovine trabecular bone from an isotropic region from the proximal humerus as a model material, we measured ultimate strengths in tension and compression for two groups of 24 specimens each. The average ultimate strength in tension was 7.6 +/- 2.2 (95% C.I.) MPa and in compression was 12.4 +/- 3.2 MPa. This difference was statistically significant (p = 0.013) and was not related to density differences between the test groups (p = 0.28). Strength was related by a power-law function of the local apparent density, but, even accounting for density influences, isotropic bovine trabecular bone exhibits significantly lower strengths in tension than in compression.

Animals↗

Teratogenic potential of inhaled dichlorobenzenes in rats and rabbits.

Orthodichlorobenzene (ODCB) and paradichlorobenzene (PDCB) were evaluated for teratogenic potential in rats (ODCB only) and rabbits. Groups of bred rats and inseminated rabbits were exposed to 0, 100, 200, or 400 ppm of ODCB; groups of inseminated rabbits were exposed to 0, 100, 300, or 800 ppm of PDCB. Animals were exposed for 6 hr/day on Days 6 through 15 (rats) or 6 through 18 (rabbits) of gestation. Maternal toxicity, as evidenced by a significant decrease in body weight gain, was observed in all groups of ODCB-exposed rats and liver weight was significantly increased in the 400-ppm ODCB-exposed group. Slight maternal toxicity was observed in groups of rabbits exposed to 400 ppm ODCB or 800 ppm PDCB as indicated by significantly decreased body weight gain during the first 3 days of exposure. Inhalation of up to 400 ppm of ODCB was not teratogenic or fetotoxic in rats, and neither ODCB nor PDCB was teratogenic or fetotoxic in rabbits at exposure levels up to 400 or 800 ppm, respectively.

Abnormalities, Drug-Induced↗

Finite element analysis of a three-dimensional open-celled model for trabecular bone.

Based on a regular array of cubic unit cells, each containing a body-centered spherical void, we created an idealized three-dimensional model for both subchondral trabecular bone and a class of porous foams. By considering only face-to-face stacking of unit cells, the inherent symmetry was such that, except at the surface, the displacements and stresses within any one unit cell were representative of the entire porous structure. Using prescribed displacements the model was loaded in both uniaxial compressive strain and uniaxial shear strain. Based on the response to these loads, we found the tensor of elastic constants for an equivalent homogeneous elastic solid with cubic symmetry. We then compared the predicted modulus with our experimental values for bovine trabecular bone and literature values for an open-celled latex rubber foam.

Bone and Bones↗

Prediction of vertebral body compressive fracture using quantitative computed tomography.

We performed quantitative computed tomography in vitro on the first and third lumbar vertebrae in human cadavera using a dibasic potassium phosphate phantom for calibration. The quantitative computed-tomography numbers exhibited a significant positive correlation (R2 = 0.89, p less than 0.0001) with direct measurements of the apparent density of the vertebral trabecular bone. We also conducted uniaxial compression tests to failure of the vertebral bodies after removal of the posterior elements, and found that vertebral compressive strength was also correlated at a high level of significance (R2 = 0.82, p less than 0.0001) with direct measurement of the trabecular apparent density. These findings suggested the possibility that the quantitative computed-tomography values might be directly predictive of vertebral compressive strength. However, when we correlated the quantitative computed-tomography values directly with vertebral compressive strength, the results (R2 = 0.46, p less than 0.061) were suggestive but not quite significant. All vertebral bodies failed by compression of the end-plate, suggesting only a modest structural role for the cortical shell under these loading conditions. This was confirmed by comparing the compressive load to failure of twenty additional pairs of vertebrae that were tested with and without an intact vertebral cortex. Removal of the cortex was associated with approximately 10 per cent reduction in vertebral load to failure.

Aged↗

Structural changes in the femur with the transition to agriculture on the Georgia coast.

Structural characteristics of the femur are compared in preagricultural (2200 B.C.-A.D. 1150) and agricultural (A.D. 1150-1550) subsistence strategy groups from the Georgia coast. Using an automated technique, cross-sectional geometric properties used in structural analyses (areas, second moments of area) were determined at midshaft and distal to the lesser trochanter in 20 adults from each group. A significant decline in magnitude of almost every geometric property occurs in the agricultural group. The differences between groups are reduced but still significant for many properties after standardizing for bone length differences. In addition, a remodeling of the femoral cortex to one of relatively smaller medullary and subperiosteal diameter, as well as a more circular cross-sectional shape, is characteristic of agricultural femora. Thus, while the relative cross-sectional area of bone remains the same, the spatial distribution of bone area is different in the two groups. The results strongly suggest a relative reduction in mechanical loadings of the femur in the agricultural group, implying different levels and possibly types of activity involving the lower limb in the two groups. The data are also compared with similar data available for the Pecos Pueblo (agricultural) sample. The comparison indicates that types of activity may have been more similar in the two agricultural samples, but that general levels of activity were more similar in the Pecos Pueblo and Georgia coast preagricultural samples.

Agriculture↗

Force ratios in the quadriceps tendon and ligamentum patellae.

We measured forces in the quadriceps tendon and ligamentum patellae in six human cadaver knee joints loaded through a range of flexion angles from 30 to 120 degrees. Using standardized loads based on one-sixth of maximum isometric quadriceps moments reported in human volunteers, we measured maximum average forces at 60 degrees flexion of 532 N in the quadriceps tendon (FQ) and 470 N in the ligamentum patellae (FL). Linear extrapolation to full maximum extensor moments results in estimates of 3,200 N for FQ and 2,800 N for FL. The force ratio (FL/FQ) reached a maximum value of 1.27 at 30 degrees and minimums of 0.7 at 90 and 120 degrees knee flexion. Contrary to prevailing opinion, our results indicate that the patella is not a simple pulley that serves only to change the direction of equal forces in the quadriceps tendon and ligamentum patellae. Instead, the ratio of forces in these structures varies significantly with flexion angle. The ratio appears to be determined by the changing location of the patellofemoral contact area relative to the insertions of the tendon and ligament. These findings emphasize the biomechanical importance of patellar length and of the vertical dimensions and locations of the patellofemoral contact area. Attempts at surgical intervention for the treatment of disorders of the extensor apparatus should recognize these variations. Procedures that tend to move the contact area more proximally (at a particular flexion angle) will also tend to decrease the FL/FQ ratio.

Aged↗

Age changes in geometry and mineral content of the lower limb bones.

Subperiosteal expansion and increase in second moments of area with aging of eleven femoral and tibial cross-sections are documented in a large archaeological sample from the American Southwest. In contrast to these geometric changes, we found little change with age in bone mineral density measured using photon absorptiometry. Thus, the most significant structural changes with age in bone appear to involve its geometry and material characteristics other than its density. Variation in age-related geometric remodeling between cross-section locations and populations may be caused by differences in mechanical stress and strain levels in vivo in the lower limb.

Adult↗

Inhalation teratology study on monochlorobenzene in rats and rabbits.

The embryotoxic and teratogenic potential of inhaled monochlorobenzene (MCB) was evaluated in rats and rabbits. Bred Fischer 344 rats and inseminated New Zealand White rabbits were exposed to 0, 75, 210, or 590 ppm of MCB via inhalation for 6 hr/day during the period of major organogenesis. Exposure to 590 ppm caused elevated liver weights in both species and decreased body weight gain and feed consumption in rats. Inhalation of MCB vapors during gestation was not embryotoxic or teratogenic in rats. In rabbits, a few MCB-exposed fetuses exhibited visceral malformations which were not observed among concurrent controls, though no dose-related increase in malformations occurred. To further evaluate the effects of MCB in rabbits, additional groups were exposed to 0, 10, 30, 75, or 590 ppm. This subsequent study did not result in any increase in malformations in the MCB-exposed groups. Fetal effects were limited to a slight delay in skeletal development which occurred only in rats exposed to 590 ppm, a maternally toxic concentration.

Abnormalities, Drug-Induced↗

Teratologic evaluation of 3,6-Dichloropicolinic acid in rats and rabbits.

3,6-Dichloropicolinic acid (clopyralid), a new herbicide, was evaluated for teratogenic potential in Fischer 344 rats and New Zealand White rabbits. Rats were given 0, 15, 75, or 250 mg clopyralid/kg/day by gavage on Days 6-15 of gestation while rabbits were given 0, 110, or 250 mg clopyralid/kg/day on Days 6-18 of gestation. Maternal toxicity, as evidenced by decreased body weight gain, was observed among pregnant rats in the 250-mg/kg/day group. No evidence of maternal toxicity was observed among treated rabbits. A teratogenic effect was not detected in either species.

Abnormalities, Drug-Induced↗

Three-dimensional finite element analysis of a simplified compression plate fixation system.

A three-dimensional, linear finite element model was generated for an intact plexiglass tube with an attached six-hole stainless steel compression plate. We examined external forces representing axial, off-center axial, and four-point bending, along with superimposed plate and screw pretension. Strain gage experiments were conducted to test model validity and the finite element results were contrasted to a composite beam theory solution. Excellent correspondence was observed between finite element and strain gage data for the most significant strain components. Composite beam theory tended to overestimate the neutral axis shift which results from plate application. The model also demonstrated fracture site distraction due to plate pretension, and the tendency for outer screw failure for the combination of bending-closed with a preload in the plate and screws.

Biomechanical Phenomena↗

Bone-mineral content in the lower limb. Relationship to cross-sectional geometry.

For this study, bone-mineral content and bone-mineral width were measured using photon absorptiometry at eleven locations in forty excised femora and tibiae obtained from an archaeological sample. An additional seventy-nine femora were scanned at the middle of the shaft and through the femoral neck. After scanning, the bones were sectioned at each location and cross-sectional areas and other geometrical properties were determined directly from section tracings. The results indicated that locational, sex, and age-related differences in bone-mineral content were largely determined by variation in cortical area. Due to differences in bone geometry, variation in bone width does not reflect variation in cortical area, and as a consequence the use of bone width to standardize for volumetric or bone "size" differences produces misleading results in sex and age comparisons. In this study, decreases with age in the bone-mineral content and bone-mineral content:bone width ratio were similar to those observed in living populations. However, the bone-mineral content:cortical area ratio showed no significant decline with age for any cross section. Thus, the age-related changes in compact cortical bone appeared to be mainly volumetric, not densitometric.

Adult↗

Patellofemoral contact pressures. The influence of q-angle and tendofemoral contact.

Controlled bending moments were applied to twelve human cadaver knee joints using a special loading fixture that allowed variation of both the Q-angle and the flexion angle. The joints were tested at three different Q-angles (physiological, increased 10 degrees, and decreased 10 degrees) and five different angles of flexion (ranging from 20 to 120 degrees). Based on one-third of values in the literature for maximum voluntary isometric quadriceps moments, we applied resultant knee moments of 23.6, 30.7, 47.2, and 35.0 newton-meters at 20, 30, 60, and 90 degrees of flexion, respectively. Based on two-thirds of reported maximum moments, we applied 47.2 newton-meters at 120 degrees of flexion. Normal patellofemoral-contact pressures, measured with a pressure-sensitive film, were remarkably uniformly distributed (+/- 0.25 megapascal ), with approximately the same pressure on the lateral and medial patellar facets. The maximum contact force occurred at 90 degrees of flexion. Extrapolating our measurements to full in vivo moments, we estimated maximum contact forces of 4600 newtons , or approximately 6.5 times body weight. Tendofemoral contact at 120 degrees of knee flexion supported one-third of the total contact force on the patella. A 10-degree increase in the Q-angle resulted in increased peak pressures (an increase of 45 per cent at 20 degrees of flexion). A decrease in the Q-angle resulted in unloading of the vertical crest and, in some knees, of parts of the lateral facet. However, these decreases were always associated with increased peak pressures (50 per cent more at 20 degrees of flexion) at other locations.

Biomechanical Phenomena↗

Cross-sectional geometry of Pecos Pueblo femora and tibiae--a biomechanical investigation: I. Method and general patterns of variation.

Variation in long bone cross-sectional geometry can be given a more precise functional interpretation using engineering beam theory. However, difficulties in measurement technique have generally prevented studies of large samples of cross sections in this way. In the present study, an automated system utilizing an electronic digitizer and computer software was used to analyze cross-sectional geometric properties of 11 femoral and tibial locations in 119 individuals from the Pecos Pueblo, New Mexico site. The data generated allow identification of clear differences in geometric properties between different regions of the femur and tibia. These differences appear to be related to specific in-vivo mechanical loadings of the lower limb bones, serving to reduce stress and strain under these loadings. The data are also used to investigate possible differences in loading of the femur and tibia in the Pecos and modern samples, and between humans and a nonhuman primate sample.

Animals↗

Cross-sectional geometry of Pecos Pueblo femora and tibiae--a biomechanical investigation: II. Sex, age, side differences.

Intra-populational variation in cross-sectional geometric properties of the femur and tibia are investigated in the Pecos Pueblo skeletal sample. Sex differences in geometric parameters suggest that male lower limb bones are more adapted for A-P bending, females for M-L bending. Proposed explanations for this finding include sexual dimorphism in pelvic structure and culturally prescribed sex-related activities at Pecos. With aging, both males and females undergo endosteal resorption and cortical thinning, greater among females. Both sexes also demonstrate an increase with age in subperiosteal area and second moments of area, supporting results reported in some studies of modern population samples. Sex and site-specific remodeling of the femur and tibia with aging also occur. These localized remodeling changes appear to selectively conserve more compact cortical bone in areas of high mechanical stress. Side differences in cross-sectional geometric properties indicate that left lower limb bones are generally larger than right lower limb bones, with asymmetry greater among females. In particular, left femora and tibiae are relatively stronger in A-P bending, again more so in females.

Adult↗

Multiaxial strength characteristics of trabecular bone.

Bovine trabecular bone specimens were tested in multiaxial stress, including pure shear, in a special test device. Shear strength was proportional to apparent density to the exponent 1.65, in approximate agreement with theoretical and experimental studies on the shear strengths of porous foams. The mean shear strength was 6.60 +/- 1.66 MPa, after normalizing for apparent density. This compares well with normalized shear strengths from Saha and Gorman's (1981) study on human femora. A scanning electron microscope study indicated random trabecular architecture and a complex fracture mechanism at the level of the individual trabecular struts. Hoffmann's (1967) 3-D isotropic failure criterion was applied to the multiaxial test data, along with data from uniaxial compression tests, indicating a compressive strength approximately three times the tensile strength.

Animals↗

Monitoring fracture site properties with external fixation.

An in-vitro system has been devised to monitor the properties of an idealized fracture site immobilized with a Hoffman-Vidal external fixator. A dial gage was used to measure the relative pin displacements under controlled axial loading. The displacement measurements were then used in conjunction with a finite element model to predict the modulus of an idealized fracture site. Five fracture sites made of neoprene disks of different mechanical properties were monitored in order to simulate the increasing modulus of a healing fracture. Good agreement was observed between directly measured mechanical properties of the neoprene and those inferred from the combined finite element and pin displacement tests.

Aluminum↗

Leg motion analysis during gait by multiaxial accelerometry: theoretical foundations and preliminary validations.

A theoretical formulation for studying limb motions and joint kinetics by multiaxial accelerometry is developed. The technique is designed to study the swing phase of human gait, modeling the lower leg as a rigid body. Major advantages of the approach are that acceleration information needed for the calculation of forces and moments is generated directly, and that the method automatically generates its own initial conditions. Results of validation experiments using both artificial and experimental data demonstrate that the method is theoretically valid, but that it taxes available instrumentation and requires further development before it can be applied in a clinical setting.

Acceleration↗