Biomedical subjects

W C K Poon

Publications and source records attributed to W C K Poon.

8 recordsLinked to original sources

PubMed2005

Non-equilibrium behavior of sticky colloidal particles: beads, clusters and gels.

To understand the non-equilibrium behavior of colloidal particles with short-range attraction, we studied salt-induced aggregation of lysozyme. Optical microscopy revealed four regimes: bicontinuous texture, 'beads', large aggregates, and transient gelation. The interaction of a metastable liquid-liquid binodal and an ergodic to non-ergodic transition boundary inside the equilibrium crystallization region can explain our findings.

Colloids↗

PubMed2004

Cluster mode-coupling approach to weak gelation in attractive colloids.

Mode-coupling theory (MCT) predicts the arrest of colloids in terms of their volume fraction, and the range and depth of the interparticle attraction. We discuss how the effective values of these parameters evolve under cluster aggregation. We argue that weak gelation in colloids can be idealized as a two-stage ergodicity breaking: first at short scales (approximated by the bare MCT) and then at larger scales (governed by MCT applied to clusters). The competition between the arrest and phase separation is considered in relation to recent experiments. We predict a long-lived "semiergodic" phase of mobile clusters, showing logarithmic relaxation close to the gel line.

Journal Article↗

PubMed2004

Crystallization of a globular protein in lipid cubic phase.

We studied the crystallization of a globular protein, lysozyme, in the cubic phase of the lipid mono-olein. The solubility of lysozyme in salt solution decreased by a factor of approximately 4 when confined in cubic phase. Monte Carlo simulations and calculations show that this can be explained by the confinement of lysozyme molecules to the narrow water cells in the cubic phase.

Crystallization↗

PubMed2004

Glasses in hard spheres with short-range attraction.

We report a detailed experimental study of the structure and dynamics of glassy states in hard spheres with short-range attraction. The system is a suspension of nearly hard-sphere colloidal particles and nonadsorbing linear polymer which induces a depletion attraction between the particles. Observation of crystallization reveals a reentrant glass transition. Static light scattering shows a continuous change in the static structure factors upon increasing attraction. Dynamic light scattering results, which cover 11 orders of magnitude in time, are consistent with the existence of two distinct kinds of glasses, those dominated by interparticle repulsion and caging, and those dominated by attraction. Samples close to the "A3 point" predicted by mode coupling theory for such systems show very slow, logarithmic dynamics.

Journal Article↗

PubMed2003

Phase behavior and crystallization kinetics of poly-12-hydroxystearic-coated polymethylmethacrylate colloids.

Polymethylmethacrylate (PMMA) colloids sterically stabilized by a layer of chemically grafted poly-12-hydroxystearic (PHSA) are widely used in experiments as model hard spheres. However, due to the coating, the interaction between particles is slightly soft. Here we report a numerical study of the effect of the PHSA coating on the phase behavior and crystallization kinetics of PMMA colloids based on parameters determined from surface-force measurements on PHSA-PMMA-coated mica surfaces [B. A. de L. Costello and P. F. Luckham, J. Colloid Interface Sci. 156, 72 (1993); B. A. de L. Costello et al., Langmuir 8, 464 (1992)]. We find that the core volume fraction of particles at freezing measured by Pusey and van Megen [Nature 320, 340 (1986)] can only be reproduced by using a thickness of the PHSA layer that is considerably larger than literature values. This may indicate that the particles are in fact slightly charged. Compared to perfect hard spheres, the crystallization rate in these slightly soft particles was found to be increased by about two orders of magnitudes.

Journal Article↗

PubMed2002

Structural aging of crystals of hard-sphere colloids.

We report a detailed experimental study of the aging of the (initial) random hexagonal close-packed (rhcp) crystals formed in suspensions of hard-sphere colloids near the melting point. By suspending the same colloidal particles in two different mixtures of solvents we are able to tune the strength of the gravitational forces acting on the particles. The crystal structure is deduced from diffraction patterns measured by the light scattering equivalent of powder x-ray crystallography. A spontaneous aging of the structure is observed over long periods of time, consisting of a fraction of pure face-centered cubic (fcc) crystals growing at the expense of the randomly stacked crystallites. The rate of growth of the new crystals is small and consistent with the predictions by Pronk and Frenkel [13]. Gravity is also revealed to affect the crystals and favor fcc order but through a slow gradual rearrangement of the random stacking. An important new observation is that small mechanical perturbations can strongly affect the structure of the colloidal crystals, promoting fcc growth and interfering with the spontaneous aging process. Previous experimental results are also discussed in the light of these new findings.

Journal Article↗

PubMed2002

Molecular segregation observed in a concentrated alcohol-water solution.

When a simple alcohol such as methanol or ethanol is mixed with water, the entropy of the system increases far less than expected for an ideal solution of randomly mixed molecules. This well-known effect has been attributed to hydrophobic headgroups creating ice-like or clathrate-like structures in the surrounding water, although experimental support for this hypothesis is scarce. In fact, an increasing amount of experimental and theoretical work suggests that the hydrophobic headgroups of alcohol molecules in aqueous solution cluster together. However, a consistent description of the details of this self-association is lacking. Here we use neutron diffraction with isotope substitution to probe the molecular-scale structure of a concentrated alcohol water mixture (7:3 molar ratio). Our data indicate that most of the water molecules exist as small hydrogen-bonded strings and clusters in a 'fluid' of close-packed methyl groups, with water clusters bridging neighbouring methanol hydroxyl groups through hydrogen bonding. This behaviour suggests that the anomalous thermodynamics of water alcohol systems arises from incomplete mixing at the molecular level and from retention of remnants of the three-dimensional hydrogen-bonded network structure of bulk water.

Journal Article↗

PubMed2002

Multiple glassy states in a simple model system.

Experiments, theory, and simulation were used to study glass formation in a simple model system composed of hard spheres with short-range attraction ("sticky hard spheres"). The experiments, using well-characterized colloids, revealed a reentrant glass transition line. Mode-coupling theory calculations and molecular dynamics simulations suggest that the reentrance is due to the existence of two qualitatively different glassy states: one dominated by repulsion (with structural arrest due to caging) and the other by attraction (with structural arrest due to bonding). This picture is consistent with a study of the particle dynamics in the colloid using dynamic light scattering.

Journal Article↗