PubMed Health⌕ Search

Biomedical subjects

W C Noble

Publications and source records attributed to W C Noble.

184 records · Page 11Linked to original sources
PubMedDate not supplied

The antigenic composition and protein profiles of eumycetoma agents.

The protein profiles of different eumycetoma agents were compared by SDS gel electrophoresis. Dendrograms confirmed the homogeneity of isolates of Pseudallescheria boydii but amongst Madurella species, particularly isolates identified as M. grisea, there were substantial differences in protein composition. However using Western blotting reference isolates of the different species showed distinct antigen patterns in response to immune rabbit sera. In particular there was little evidence of cross reactivity between M. mycetomatis and M. grisea. However this specificity was not apparent when human sera from patients with different eumycetoma infections were compared in an ELISA system using the same antigens. It is possible that the formation of a mycetoma grain may limit a patient's exposure to antigens which confer specificity, an explanation which may also account for the variability in antibody responses seen.

Antigens, Fungal↗
PubMedDate not supplied

Polyacrylamide gel electrophoresis patterns of some Microsporum species.

The paper reports observations made of various Microsporum species during a PAGE pattern study of the epidemiology of Microsporum canis. It is shown that M. audouinii can readily be distinguished from M. canis but that M. equinum is more difficult to separate. M. rivalierii was distinguishable from M. audouinii, confirming previous reports. M. distortum could not be separated from M. canis by PAGE gels of total protein or by isozyme patterns generated. There are particular problems with studies of that type on dermatophyte protein in that the intrinsic proteases degrade the preparations.

Electrophoresis, Polyacrylamide Gel↗