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W C Noble

Publications and source records attributed to W C Noble.

At least 109 records · Page 6Linked to original sources
PubMed1984

Gas chromatography-mass spectrometry of mycolic acids as a tool in the identification of medically important coryneform bacteria.

The mycolic acid derivatives of 11 unidentified pathogenic coryneform bacteria were examined by TLC, GLC and GLC-mass spectrometry. The resulting mycolic acid profiles of the unidentified isolates were compared with those of type or reference strains of possibly related coryneform species, namely Corynebacterium bovis, C. diphtheriae, C. xerosis and Rhodococcus equi. It was apparent that most of the unidentified strains showed a distinctive mycolic acid profile, with predominant amounts of relatively high molecular weight mycolic acids (C32-C36) and a high degree of unsaturation, and could thus be distinguished from both C. bovis, which had exceptionally low molecular weight mycolic acids (C24-C30), and C. diphtheriae (C28-C34), which had large amounts of saturated mycolic acids. The mycolates of C. xerosis and R. equi (C28-C36) were generally similar to those of the unidentified coryneforms but their overall mycolic acid patterns were different from one another as well as the unidentified strains. The mycolic acid profiles exhibited by the pathogenic coryneforms examined here were very similar to one another but unlike that of any of the type or reference strains included in the study.

Actinomycetales↗
PubMed1984

Plasmids of phage-Group-II Staphylococcus aureus.

Most phage-Group-II Staphylococcus aureus have been shown to carry at least one plasmid. The proportion of strains that are resistant to tetracycline appears to have increased during the last 17 years. Restriction maps of several of the small plasmids isolated from Group-II strains are presented and compared with those known for staphylococcal plasmids. These small plasmids are similar to previously characterised plasmids from staphylococci of other phage groups.

Bacteriophage Typing↗
PubMed1984

Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns.

A total of 57 strains of Acinetobacter calcoaceticus was fingerprinted by SDS-PAGE of cellular protein. All strains were also examined by conventional, API and N/F-Tek methods, and antibiotic sensitivity patterns were determined. In general, using the API 20E and N/F-Tek methods, it was possible to assign isolates of A. calcoaceticus to the two accepted "biotypes" or "varieties", A.c. anitratus and A.c. lwoffi, but these methods did not offer useful subdivision of the biotypes. Gel electrophoresis permitted subdivision of the strains into clusters in a manner which suggests that the technique may be valuable in typing strains isolated during outbreaks of infection in hospital.

Acinetobacter↗
PubMed1984

Colonization of the axilla by Propionibacterium avidum in relation to age.

The prevalence of Propionibacterium avidum in the axillae of 248 individuals aged 5 to 18 years was studied. Both caucasoids and negroids, males (132) and females (116), were included. The frequency of recovery of the organism increased with age and was associated with the onset of puberty. The organism was recovered from only 2 of 40 females (5%) aged 5 to 10 years and from 1 of 22 males (4.5%), whereas 45% of females and 58% of males aged 15 to 16 years were colonized. Females tended to become colonized at an earlier age than males did. It appeared that negroids carried the organism more frequently than caucasoids, but the difference in prevalence was not statistically significant in matching age groups. It is suggested that the increase of hydration of the axilla with age and increase in substrate availability play an important part in the colonization process.

Adolescent↗
PubMed1983

Gentamicin-resistant staphylococci: genetics of an outbreak in a dermatology department.

The genetics of gentamicin resistance in Staphylococcus aureus strains isolated during an outbreak of infection in dermatology department have been studied. The predominant strain of S. aureus did not appear to possess a plasmid mediating gentamicin resistance though one isolate yielded a plasmid coding for penicillin and gentamicin. Three distinct plasmids were isolated from other phage types of S. aureus which appeared towards the end of the epidemic. There appeared to be a stepwise loss of gentamicin resistance in the predominant strain.

Aminoglycosides↗
PubMed1983

Association between histocompatability antigens (HLA) and nasal carriage of Staphylococcus aureus.

We investigated the association between phenotypes of histocompatability antigen (HLA) and nasal carriage of Staphylococcus aureus in two populations--healthy laboratory workers and patients attending an outpatients' clinic. When data from the two sources were pooled, it was evident that the presence of HLA-DR3 was associated with carriage, and the presence of HLA-DR2, HLA-DR1 and HLA-Bw35 with lack of carriage. However, since each person may have two antigenic specificities encoded at the HLA-A, the HLA-B, and the HLA-DR loci, the carriage of the organism was analysed for paired combinations of the more frequent phenotypes. For example, the lack of carriage evident with HLA-DR1 was more marked with the DR1-A11 and DR1-B7 combinations while the predisposition towards carriage shown with HLA-DR3 was more marked with the DR3-DR5 combination. The importance of the analysis of antigen combinations is discussed in relation to association of single antigens with carriage of S. aureus.

Adult↗
PubMed1983

Fusidane antibiotics produced by dermatophytes.

Isolates of Microsporum canis, Microsporum gypseum and Epidermophyton floccosum were observed to produce antibacterial activities under cross-resistance to fusidic acid. The activity from E. floccosum was shown to be due to fusidic acid, diketofusidic acid and 3-ketofusidic acid. Possible contributions of these antibiotics to microbial interaction during dermatophytosis is discussed.

Anti-Bacterial Agents↗
PubMed1983

Bacteria and fungi in severe foot infection.

In a study of severe foot infection in 21 miners, an attempt was made to match nine clinical parameters with both bacteriological and mycological findings. Erythema was significantly more pronounced in the presence of dermatophytes but less pronounced in the presence of Gram-negative bacilli. No other clinical parameter differed in relation to the presence of particular microorganisms.

Bacteria↗
PubMed1983

The selection of penicillin-resistant staphylococci on murine skin.

The survival of staphylococci on mouse skin and on glass has been studied using paired isolates, isogenic but for a penicillinase plasmid. In general, there is a slight tendency for the penicillin-resistant isolates to survive better than the sensitive variants. In one staphylococcus it was possible to compare the effect of two different penicillinase plasmids. One gave a slightly enhanced survival but the other was detrimental to the host cell's survival. The effect seems not to be related to desiccation or to the effect of lipid.

Animals↗
PubMed1982

An electrophoretic study of enzymes as a tool in the taxonomy of the dermatophytes.

Zymogram patterns from 84 strains of dermatophyte fungi were obtained using polyacrylamide gradient gel electrophoresis of total cell protein extracts. The enzymes investigated were alpha-naphthyl acetate esterase, acid phosphatase, lactate dehydrogenase, malate dehydrogenase, tetrazolium oxidase and catalase. These patterns were used to construct similarity matrices and dendrograms using computerized techniques. The results showed that zymograms allowed some species to be readily recognized despite morphological variation. This was also seen in the dendrograms where, in addition, groupings based on ecological or sexual criteria could be distinguished.

Arthrodermataceae↗
PubMed1981

Transfer of gentamicin resistance between coagulase-negative and coagulase-positive staphylococci on skin.

The transfer of gentamicin resistance between a coagulase-negative S. hominis strain and various coagulase-positive S. aureus strains on human and murine skin in the absence of a selective agent is described. Transfer occurs at higher frequency on skin than in broth. Skin transfer may account for the apparently explosive occurrence of gentamicin resistant staphylococci in hospitals.

Animals↗
PubMed1981

Interactions between dermatophyte fungi and staphylococci or Brevibacterium in vitro.

Interactions between dermatophyte fungi and staphylococci or brevibacteria on a new skin-based culture medium are described. Penicillin production by the fungus selects penicillin resistant S. aureus or B. epidermidis. Fungi are inhibited by brevibacteria but not by the staphylococci. "Keratolysis" by fungi may contribute to the growth nutrients of staphylococci.

Arthrodermataceae↗