Biomedical subjects

W Cottle

Publications and source records attributed to W Cottle.

5 recordsLinked to original sources

PubMed2001

Does anterolateral cage insertion enhance immediate stabilization of the functional spinal unit? A biomechanical investigation.

STUDY DESIGN: The three-dimensional flexibility of six human lumbar functional spinal units was measured after the anterolateral insertion of an interbody cage. OBJECTIVES: To determine whether an interbody cage inserted from an anterolateral direction stabilizes the spine with respect to the intact state and to compare the finding with that from the same cage inserted from an anterior direction. SUMMARY OF BACKGROUND DATA: Several biomechanical studies have shown that interbody cages do not stabilize the spine in extension. It is suspected that this may be caused by the destruction of the anterior longitudinal ligament and anterior anulus fibrosus. METHODS: Six human cadaveric lumbar functional spinal units were tested under pure moments of flexion, extension, bilateral axial rotation, and bilateral lateral bending to a maximum of 10 Nm. The relative intervertebral motions were measured by an optoelectronic camera system with the spinal units in the intact condition, after discectomy, after anterolateral interbody cage stabilization, and with additional translaminar screw fixation. The implant used was a central, porous, contoured implant with endplate fit. The results were compared with those of a previous study, which used the same implant inserted from an anterior direction. RESULTS: The anterolateral cage insertion significantly decreased the motion in comparison with the intact situation in flexion and lateral bending, but not in extension or axial rotation. No differences were found between the anterior and anterolateral insertion approaches in flexion or extension, but differences were observed in axial rotation and lateral bending, in which the anterolateral approach resulted in more motion. Additional translaminar screw fixation reduced motion to below intact levels in all loading directions. None of the surgical procedures introduced asymmetrical behavior. CONCLUSIONS: Anterolateral cage insertion did not stabilize the spine in extension or axial rotation and was not different from the anterior approach in flexion and extension. Additional translaminar screw fixation stabilized in all directions.

Biomechanical Phenomena↗

PubMed1995

A transpedicularly implanted anterior spinal support.

This article describes a device that has been developed to augment posterior spinal fracture fixation by providing support for the anterior column of the spine. The device is contracted for insertion through the limited opening provided by the transpedicular approach to the vertebral body. Once in situ it is configured to support physiological loads. In this way the structural limitations of posterior instrumentation and the surgical difficulties of anterior spinal fixation instrumentation are overcome. A preliminary investigation of the new device together with in vitro studies of a prototype are described in this report. In vitro tests to determine the expansion characteristics and structural performance of the device were performed together with tests to determine the efficacy of the support to reduce loading on the posterior fixation device. It is shown that the device has the necessary expansion characteristics and can support physiological loads without failure. Tests using porcine vertebral columns with a simulated vertebral fracture showed a significant reduction in load carried by the posterior fixation plates (44 +/- 16%) when the anterior support was included in the construct. In addition, the difference in loading between the left and right plates was significantly reduced (81 +/- 12%).

Bone Cements↗

PubMed1987

Are indices of free radical damage related to exercise intensity.

The possibility that plasma levels of malonaldehyde (MDA) are altered by exercise has been examined. The presence of MDA has been recognized to reflect peroxidation of lipids resulting from reactions with free radicals. Maximal exercise, eliciting 100% of maximal oxygen consumption (VO2max) resulted in a 26% increase in plasma MDA (P less than 0.005). Short periods of intermittent exercise, the intensity of which was varied, indicated a correlation between lactate and MDA (r2 = 0.51) (p less than 0.001). Blood lactate concentrations increased throughout this exercise regimen. A significant decrease (10.3%) in plasma MDA occurred at 40% VO2max. At 70% VO2max plasma MDA was still below resting values, however the trend to an increase in MDA with exercise intensity was evident. At exhaustion, plasma MDA and lactate were significantly greater than at rest. These results suggest, that exhaustive maximal exercise induces free radical generation while short periods of submaximal exercise (i.e. less than 70% VO2max) may inhibit it and lipid peroxidation.

Adult↗

PubMed1982

Biosynthesis, deposition, and partial characterization of potato suberin phenolics.

Alkaline nitrobenzene oxidation of the polymeric materials from wound-healed potato (Solanum tuberosum L. var. White Rose) tuber tissue liberated p-hydroxybenzaldehyde, vanillin, and minor amounts of syringaldehyde as determined by gas chromatography/mass spectrometry. The aromatic aldehydes were derived only from periderm. The amounts of aromatic aldehydes liberated were used as a measure of the deposition of phenolic suberin components. Phenolic deposition began after about 2 days of wound healing; after 8 days the amounts of p-hydroxybenzaldehyde released by nitrobenzene oxidation leveled off at 5 milligrams per gram dry weight and after 12 days vanillin liberation reached a maximum at 7.5 milligrams per gram dry weight. The time course of deposition of the phenolic polymeric material is analogous to that reported for the deposition of the aliphatic components of suberin and therefore these results are consistent with the proposed structure of suberin. Experiments with radiolabeled l-phenylalanine and cinnamic acid indicated that exogenous phenylalanine was less efficient than cinnamic acid as a precursor of suberin phenolics. Nitrobenzene oxidation of radiolabeled suberin preparations gave three major labeled fractions: a diethyl ether-soluble fraction containing aromatic aldehydes ( approximately 20%), an ethyl acetate-soluble fraction containing unknown compounds ( approximately 15%), and a condensed phenolic fraction ( approximately 10%). Thin-layer and gas-liquid chromatographic analysis of the ether fraction showed that the major labeled components were vanillin and p-hydroxybenzaldehyde. The condensed tannin fraction revealed the presence of several labeled macromolecular phenolic fractions. Elution profiles of the condensed tannin fraction from tissues suberized for different periods of time were essentially identical, suggesting qualitative similarity of deposition and polymerization of suberin phenolics throughout the duration of wound healing. Chlorogenic acid accumulation in wound healing potato tuber discs was measured by high-performance liquid chromatography. The level of this compound reached 130 micrograms per disk after 11 days and did not decline even after the deposition of suberin ceased, revealing no precursor role for this acid in suberization.

Journal Article↗

PubMed1982

Abscisic Acid stimulation of suberization : induction of enzymes and deposition of polymeric components and associated waxes in tissue cultures of potato tuber.

Effect of abscisic acid (ABA) on suberization of potato (Solanum tuberosum var. Russet-Burbank) tuber tissue culture was studied by measuring deposition of suberin components and the level of certain key enzymes postulated to be involved in suberization. ABA treatment resulted in a 3-fold increase in the polymeric aliphatic components of suberin and a 4-fold increase in the polymeric aromatic components. Hydrocarbons and fatty alcohols, two components characteristic of waxes associated with potato suberin, increased 9- and 5-fold, respectively, as a result of ABA treatment. Thus, the deposition of the polymeric aliphatics and aromatics as well as waxes, all of which have been postulated to be components of suberized cell walls, was markedly stimulated by ABA. omega-Hydroxy-fatty acid dehydrogenase which showed a rather high initial level of activity increased only 60% due to ABA treatment. Phenylalanine ammonia-lyase activity reached a maximum at a 5-fold level after 4 days in the ABA medium, whereas the control showed only a 3-fold increase. ABA treatment also resulted in a dramatic (7-fold) increase in an isozyme of peroxidase which has been specifically associated with suberization. Thus, ABA appears to induce certain key enzymes which are most probably involved in suberization.

Journal Article↗