Biomedical subjects

W D Gassel

Publications and source records attributed to W D Gassel.

At least 37 records · Page 2Linked to original sources

PubMed1978

[The agar culture of the leucopoietic stem cell (CFU-C) and its stimulation (author's transl)].

This review summarizes the different in vitro culture techniques of the leukopoietic stem cell. Mainly the results obtained by the system of Pluznik and Sachs and Bradley and Metcalf are described apply with semisolid agar and its modification by Iscove using methylcellulose. Furthermore, recently published modifications of culture technique are listed. The principal results obtained with mouse and human bone marrow cells are summarized with respect to the origin of the colony-stimulating factor acting in different species, its biochemical characteristics, as well as with regard to the colony forming cell and its different subclasses.

Animals↗

PubMed1978

The influence of a new antitumor agent on hemopoietic colony forming cells.

The cytostatic and immunsuppressive agent N'-methyl-N'-beta-chloroethylbenzaldehyde hydrazone (B1) in in-vitro experiments has a stimulating effect on colony-forming culture (CFUc) of bone marrow from C57BL mice. This unusual behaviour, which is in contrast to other cytostatics, could also be observed in vitro with CFUc obtained from mice treated with therapeutic doses of B1 for 2 weeks. This stimulation is not a particular effect of B1 alone but seems to depend on a synergistic effect of the combination of B1 and the colony-stimulating activity (CSA) present in the serum from endotoxin-treated mice (MP) in the testing system. The results suggest that the described effect of B1 is due to an interference at the cell membrane of CFUc or their precursor cells.

Antineoplastic Agents↗

PubMed1978

Preparation of colony stimulating activity from large batches of human urine and production of antisera against it.

In vitro induction of myelopoetic colonies from mouse bone marrow has been used for measurement of leucopoetic colony stimulating activity (CSA) isolated from large batches of human urine. After high flow dialysis in artificial kidneys and immediate adsorption to DEAE-Cellulose, followed by purification on Con A-Sepharose, treatment with insoluble Papain and gelfiltration on Sephadex G 100, enrichment of CSA was about 6,000-fold. An important step of the enrichment procedure was the separation from a CSA-inhibiting protein, probably combining with CSA. Specific activity was further increased by preparative polyacrylamide gel electrophoresis to 5.3 X 10(6) units per mg protein. The total enrichment exceeded 25,000-fold. The final purification product consisted of a group of closely related proteins with high specific activity. Antisera raised with one of the electrophoretic fractions suppressed bioactivity in each of the different purification steps including the final CSA fractions differing in electrophoretic mobility. The antisera furthermore inhibited CSA in human lung and monocyte conditioned media but had only very little effect on partially purified CSA from stimulated human lymphocytes as well as CSA derived from mouse lung conditioned medium.

Chromatography, Gel↗

PubMed1978

Effect of neutral proteases from human granulocytes on colony forming cells in vitro.

Marked inhibition of colony formation is observed after incubation of mouse and human bone marrow cells with the human granulocytic neutral proteases elastase and chymotrypsin as well as with pancreatic chymotrypsin. The corresponding enzymes inactivated with diisopropylfluorophosphate were almost inactive. Incubation of different colony inducing agents either resulted in no change or in an increase of their colony stimulating activity. The data suggest a direct proteolytic action of the proteases on colony forming cells which may alter receptor sites for colony stimulating activities.

Animals↗

PubMed1978

[Contrast media and renal function in multiple myeloma (author's transl)].

An acute deterioration of the renal function after injection of (diiodized) renal contrast media in patients with multiple myeloma was pointed out for the first time by Bartels et al. (1954). Since then only a few similar cases have been reported. Therefore in a retrospective study the records of 89 patients with multiple myeloma from 1959 to 1974 were evaluated. For 31 of these patients a total of 41 i.v. pyelographs was made. Corresponding to a previous study by Vix in none of our patients who underwent pyelography a reduction of renal function was observed in direct association with the administration of contrast media. We are in agreement with other authors that a recognized myeloma is not from the first a contra-indication for the application of contrast material containing iodine so as a dehydration of the patients can be carefully avoided.

Acute Kidney Injury↗

PubMed1978

Colony stimulating factor (CSF) in human mixed lymphocyte cultures: effect on human and mouse progenitor cells.

Supernatants from human two-way MLC, poor and rich in monocytes, were tested for their ability to induce colony growth of human and mouse progenitor cells in semi solid agar. Colony stimulating factor (CSF), with activity in both systems, indicated that allogenic lymphocytes require monocytes to produce CSF. Whereas human marrow showed an early kinetics of production, the liberation of CSF active on mouse marrow cells exhibited a delayed kinetics. These results, combined with other evidence, suggest that human lymphocytes produce two different factors.

Bone Marrow Cells↗

PubMed1977

[Combination chemotherapy in patients with disseminated bronchogenic carcinoma (author's transl)].

49 patients with disseminated bronchogenic carcinoma (small cell, 16;squamous cell, 17;large cell, 12;adeno, 4) were treated with a combination of adriamycin, cyclophosphamide, vincristine and DTIC. In a randomized series (21 patients) chemotherapy alone was compared to chemotherapy plus heparin. Partial remission was achieved in 8 patients with small cell carcinoma, in 5 patients with large carcinoma and in 2 patients with squamous cell carcinoma. The survival of patients who responded to therapy was greater than the survival of patients who did not respond to therapy. The duration of remission and the survival time was not longer in patients who had additional heparin therapy.

Antineoplastic Agents↗

PubMed1976

[Lymphocyte antigens in autoimmune thrombocytopenia].

18 patients with proven autoimmune thrombocytopenic purpura (ATP) (group A) were screened for human lymphocyte antigens (HLA) and compared with 13 patients with secondary thrombocytopenic purpura (group B). An additional comparison was made with a control panel of a European population (group C). HLA-B8 was present in 12 of 18 patients (66.6%) of group A, compared with only one of 13 patients (7.6%) of group B. HLA-B12 occured in 8 of 18 patients (44.4%) of group A, compared with, again, only one of 13 patients in group B. The alleles HLA-B8/B12 were observed in 7 of 18 patients (38.8%) of group A, whereas no patient of group B exhibited such phenotypes. The differences were statistically significant (p less than 0.001 to p less than 0.03). A high incidence of the antigens HLA-B8/B12 has been noted in a family of which three members displayed serious symptoms of ATP over three generations. Thus, it is conceivable that individuals who carry the HLA-B8/B12 alleles run an increased risk of developing the disease if they are exposed to additional causal factors which finally trigger ATP.

Adolescent↗

PubMed1976