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Biomedical subjects

W Dummler

Publications and source records attributed to W Dummler.

At least 37 records · Page 2Linked to original sources
PubMed1987

[Studies of pure human pancreatic juice].

The test-combination from Fa. Boehringer Mannheim GmbH for measuring citric acid present in foodstuffs is also suitable for use in human pancreatic juice. Accuracy, within-run and between-day imprecision are satisfactory. The detection limit of the method is at least 10 mumol citrate/l pancreatic juice. The limit can be reduced when exact additions of citrate are given to the pancreatic juice and then evaluated using regression analysis. The presence of Ca-ions and possibly also trypsin in the material to be tested does not interfere with the reaction. It is, however, necessary to remove proteins from the sample. Deproteinization can be performed either by ultrafiltration or with perchloric acid.

Cholecystokinin↗
PubMed1986

[Biochemical diagnosis of duodenitis].

The activities of enteropeptidase, alanine aminopeptidase, sucrase, and leucine aminopeptidase were determined in mucosa biopsies taken from three defined places of the duodenum and in duodenal juice. We examined 23 adults with a histological proven normal mucosa and 10 patients suffering from duodenitis grade I. Using multivariate evaluation of all the four enzyme activities of the three mucosa sites, we could differentiate duodenitis from normal mucosa with an efficiency of 88%.

Adult↗
PubMed1983

Immunohistochemical localization of trypsinogen and trypsin in acute and chronic pancreatitis.

Immunoreactive trypsin was localized with the peroxidase-antiperoxidase technique in normal human pancreatic tissue and in the glands of patients suffering from acute or chronic pancreatitis. In the normal pancreas and in the histologically normal areas of the inflamed pancreas, trypsin was detected in the zymogen granules of acinar cells and in ductal secretory material. During acute pancreatitis, three characteristic changes were observed: (1) separate acinar cell fragments in early lesions; (2) decreased and evenly dispersed staining in necrotic acinar cells, and (3) intensive reaction in plugs in acinar lumina in advanced lesions. In chronic pancreatitis, the localization of trypsin in acinar cells was similar to that in normal pancreas. Some proteinaceous plugs in dilated pancreatic ducts were weakly immunoreactive. The results show that the tissue distribution of immunoreactive trypsin is altered in acute pancreatitis.

Acute Disease↗
PubMed1982

[Gastroesophageal reflux and gastric secretion].

In 47 patients with reflux complaints and pH-metrically recognizable gastrooesophageal reflux (glass-calomel-electrode system) we succeeded in proving the fact that a large volume of gastric secretion and a lower intragastric pH-value are concomitant with a significant prolongation of the total reflux duration by increase of the reflux frequency.

Female↗
PubMed1982

[Reflux disease and gastric acid secretion].

Between the patients with peptic oesophagitis (n = 28), typical reflux complaints without oesophagitis (n = 60) and pH-metrically establishable gastrooesophageal reflux (n = 58) as well as adequate control groups on an average no differences were the result for the volume of the gastric secretion estimated by aspiration, the concentration of hydrogen ions in the aspirated gastric juice and the computed secretion function of the stomach under basic and stimulation conditions as well as for the pH-value established in the stomach under fasting conditions by means of a glass electrode which can be swallowed.

Esophagitis, Peptic↗
PubMed1982

[Pancreatic findings after ductal occlusion in rats. A histological and biochemical study].

By histological examination of the exocrine pancreas a triphasic reaction was proven in 46 rats of a wistar strain 1 hour up to 8 weeks after a transduodenal intraductal instillation of an alcoholic amino acid solution (Ethibloc). The first phase, due to both the incorporated substance by itself and an acutely increased intraductal pressure, consists in an interstitial edema with transition into a perilobular pancreatitis, a cytotoxic damage of the acinar and ductal epithelium with focal necrosis of parenchyma and fat-tissue, a sialangiitis and foreign body granulomas. In a second phase, produced by the occlusion of the duct system, a cystofibrosis of the lobuli developed within 7 to 14 days after a preceding hyperplasia and ectasia of intercalated ducts and an atrophy of acini. In a third phase of pancreas injury, starting at the 14th day of experiment, in non-atrophied lobuli a progressive intralobular, supposed autoimmunological, pancreatitis developed. Changes in the activity of serum lipase and -amylase correlated strongly with the morphological findings. The results suggest that at last a nearly complete burnout of the normal rat pancreas is possible by duct occlusion. But for the clinical application the danger of provocation of an acute relapse of chronic pancreatitis might be given by the presence of a secreting parenchyma.

Amylases↗
PubMed1980

[Determination of alanine aminopeptidase and lactate dehydrogenase in the urine as a control over the patient's course after kidney transplantation].

In 42 patients the determination of alanine aminopeptidase and lactate dehydrogenase in the urine was performed after transplantation of a kidney. In the evaluation of the enzyme activities it was referred to the clinically made rejection diagnosis as well as to the increase of the serum creatinine. We found out that with regard to the clinically made rejection diagnosis the alanine aminopeptidase in 56% and the lactate dehydrogenase in 55% of the cases showed an increase before the beginning of the rejection therapy. Before the corresponding increase of the serum creatinine we could establish an increase of the enzyme activity in the alanine aminopeptidase in 45% and in the lactate dehydrogenase in 65% of the cases. For a better interpretation of the urinary enzyme activities during the rejection crises the average activities of the enzymes immediately after the dismissal of the patients as well as three months, six months and one year after the transplantation are cited. From our investigation results that the daily determination of alanine aminopeptidase and lactate dehydrogenase in the urine is suited, taking into consideration other possible influence factors on the enzyme activity as well as other parameters, to support the diagnostics in finding the rejection after the transplantation of a kidney.

Aminopeptidases↗
PubMed1980

[Exocrine pancreatic function and endoscopic retrograde pancreatography. A comparative evaluation (author's transl)].

The authors have performed successively endoscopic retrograde pancreatography (ERP) and secretin-pancreozymin test (SPT) 104 times. A good correlation between ERP und SPT was demonstrated in 84.6%: 50% of patients with pathologic pancreatogram showed also an abnormal pancreatic function test while 36 (34.6%) out of 104 examinations demonstrated both normlal ERP und SPT. A discrepancy between both procedures was found only in 15.4%: pathologic ERP and normal SPT (9.6%) and reversal (5.8%). In conclusions, ERP and pancreatic function tests appear complementary to each other in the evaluation of pancreatic disorders.

Cholangiopancreatography, Endoscopic Retrograde↗
PubMed1979

[Modern pancreatic function tests].

The functional diagnostics is a corner-pillar of the difficult diagnostics of pancreas. Despite new tests many wishes remain open. The secretine-pancreozymine test and the Lundh-test give good informations, but they are expensive and for the patients considerably stressing. They certainly are not regarded as screening tests. The suitable and justifiable tests for epidemiologic examinations (estimations of stool enzymes and of serum isoamylase) are less specific and sensitive. A differentiation between chronic pancreatitis and neoplasm of the pancreas is not possible with the help of the functional diagnostics. The results of functional examinations may be correctly evaluated only within all informations which concentrate at the patient's bed.

4-Aminobenzoic Acid↗
PubMed1978

[Hemodialysis and metabolic disorders].

On the basis of investigations of the water and electrolyte balance, of the protein balance, the lipid metabolism and the hormone metabolism at the instance of aldosterone a concept of specific dialysis-conditioned pathophysiological disturbances is developed in patients undergoing a chronic programma of dialysis. The changes of metabolism induced by apparative therapy have a decisive influence on the pathophysiological mechanisms in the uraemic organism and condition a specific pathophysiology of the patient undergoing dialysis.

Aldosterone↗