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Biomedical subjects

W F Butler

Publications and source records attributed to W F Butler.

34 records · Page 2Linked to original sources
PubMed1976

Uridine diphosphoglucose dehydrogenase in the intervertebral disc.

Intervertebral discs of an old sheep and a young pig were examined for the presence of cells containing the enzyme uridine diphosphoglucose dehydrogenase. In the sheep, the inner anulus had a higher proportion of active cells than the outer anulus; in the pig, there was no difference. From a consideration of cell numbers, it is suggested that there is an accumulation of glycosaminoglycans in the centre of the disc rather than an increased production rate. Notochordal cells in the pig disc contain uridine diphosphoglucose dehydrogenase and are capable of producing glycosaminoglycans.

Alcohol Oxidoreductases↗
PubMed1975

Glycosaminoglycans of hair follicles of dog skin.

Alcian Blue staining in MgCl-2 of various concentrations revealed that the basement membrane of dog hair follicles contains a large amount of glycosaminoglycan that increases with age, varies with breed, and is significantly greater than that of dermal collagen. This material is highly sulphated and of low molecular weight. Glycoprotein is also present in significantly greater amount than in dermal collagen. Active hair matrix contains clycosaminoglycan in similar amounts to epidermis but the glycoprotein content is much greater and staining is abolished in the keratinized cortex.

Aging↗
PubMed1975

Fragility of the skin in a cat.

Skin that showed abnormal fragility was investigated by histochemical and other methods. There was a reduction in the amount of acid glycosaminoglycan but collagen cross-linkage and electron microscopic appearance of the fibres was normal. This differs from the condition of dermatosparaxis noted in other species.

Animals↗
PubMed1971

Glycosaminoglycans of cat intervertebral disc.

The glycosaminoglycan contents of samples from cat intervertebral discs were examined by using cetylpyridinium chloride salt elution techniques. The values obtained related to the region of the vertebral column from which they were derived, to the area of the disc, and to water content. In wet tissue there was a significant difference between regions of the vertebral column and between areas of the disc and findings agreed with previous histological reports. The greater part of the glycosaminoglycans present consisted of chondroitin sulphate and dermatan sulphate with smaller amounts of hyaluronic acid; little keratan sulphate was found. The maximum amounts of chondroitin sulphate and dermatan sulphate occurred in the 0.5m-magnesium chloride fractions usually, but moved towards higher molar concentrations in samples derived from some sites, particularly in the lumbar region. Mean values for the water content of the areas of the disc were: nucleus pulposus, 82.4%; inner anulus, 65.6%; outer anulus, 50.5%. The water content was directly related to the amounts of chondroitin sulphate and dermatan sulphate.

Animals↗