A study was made of deoxynivalenol (DON) incidences and levels in 1982 hard red winter (HRW) wheat grown in areas of Nebraska and Kansas known to have scabby wheat. Samples of wheat harvested in the areas were collected from elevators and analyzed for DON by gas chromatography with an electron capture detector. Of the 161 samples analyzed, 42% contained less than or equal to 1 ppm; 68% contained less than or equal to 2 ppm; 90% contained less than or equal to 4 ppm. There were differences in the occurrence of DON in the 5 areas identified in eastern Nebraska and Kansas. The mean level of DON decreased from north to south in these areas in the following order: 2.81, 2.73, 2.05, 1.52, and 0.83 ppm. An area in north central Kansas had a mean level of DON of 0.50 ppm. Correlations were made between DON incidences and levels in HRW wheat and factors used in grading wheat. The occurrence of DON was highly correlated with percent total kernels damaged by mold, percent total defects, and percent total scab damage.

Chromatography, Gas↗

PubMedDate not supplied

Liquid chromatographic determination of alpha-zearalenol and zearalenone in corn: collaborative study.

The liquid chromatographic determination of alpha-zearalenol and zearalenone in corn was collaboratively studied. Each of 13 collaborators received 7 corn samples; 2 were blanks and 5 were spiked to contain 50, 100, and 200 ng alpha-zearalenol/g and 50, 100, 500, 1000, and 4000 ng zearalenone/g. Four sets (including blanks) of blind duplicates were included in the study. Five naturally contaminated corn samples (one in duplicate) were also provided. All collaborators detected both mycotoxins at 50 ng/g. Average recoveries reported by all collaborators ranged from 81.9% at 200 ng/g to 100.3% at 50 ng/g for alpha-zearalenol and from 77.8% at 1000 ng/g to 123% at 50 ng/g for zearalenone. Three collaborators reported false positives for both alpha-zearalenol and zearalenone. The within-laboratory CV values based on blind duplicates were 22.6% for alpha-zearalenol and 31.4% for zearalenone. The CV values based on laboratory-sample interaction were 25.6 and 33.8% for alpha-zearalenol and zearalenone, respectively. The CV values for naturally contaminated samples (including duplicates) were 47.0% for alpha-zearalenol and 37.7% for zearalenone. The method has been adopted official first action.

Chromatography, Liquid↗

PubMedDate not supplied

Rapid liquid chromatographic determination of aflatoxins M1 and M2 in artificially contaminated fluid milks: collaborative study.

An international collaborative study involving 14 collaborators from 5 different countries was conducted to test a rapid liquid chromatographic (LC) method for detecting aflatoxins M1 and M2 in fluid milk. Each collaborator prepared artificially contaminated milk samples (0.078-1.31 ng M1/mL and 0.030-0.13 ng M2/mL) by adding solutions containing various concentrations of aflatoxins M1 and M2 to fresh milk. Recoveries ranged from 85.2 to 102.5% (av. 93.7%) for aflatoxin M1 and from 99.5 to 126.7% (av. 109.8%) for aflatoxin M2. Coefficients of variation averaged 21.4% (M1) and 35.9% (M2). An analysis of variance was calculated from combined data to determine variance components. The within-laboratory variations (So) (repeatability) were 27.9% (M1) and 23.9% (M2), and the among-laboratory variations (Sx) (reproducibility) were 44.5% (M1) and 64.7% (M2). No visual differences were determined between normal or reverse phase LC for contaminated samples; however, there were an insufficient number of collaborators using normal phase to give meaningful separate statistical data. For 26 observations of uncontaminated milk, 3 false M1 positives were reported for normal phase LC determinations and 2 false M1 positives were reported for reverse phase LC determinations. Three normal phase and 11 reverse phase false M2 positives were reported for 104 observations in uncontaminated milk. The reverse phase LC method for determination of aflatoxins M1 and M2 in fluid milk has been adopted official first action.

Aflatoxin M1↗