PubMed Health⌕ Search

Biomedical subjects

W F NOYES

Publications and source records attributed to W F NOYES.

13 recordsLinked to original sources
PubMed1959

Studies on the Shope rabbit papilloma virus. II. The location of infective virus in papillomas of the cottontail rabbit.

A method has been devised to determine the location of infective Shope virus in the papillomas of cottontail rabbits. Frozen sections of the growths were burned selectively with a microcautery to destroy either the keratinized or proliferating layer and the sections were then applied directly to the sensitized epidermis of domestic rabbits. No papillomas appeared when the keratohyaline and keratinized areas had been eliminated leaving the proliferating cell layer, whereas papillomas arose when the proliferating cell areas were destroyed leaving the keratohyaline and keratinized layers. The results indicate that infective Shope papilloma virus is situated mainly, perhaps entirely, in the keratohyaline and keratinized areas of cottontail papillomas. This is in accord with the previous disclosure by the fluorescence technique that virus antigen in demonstrable quantity is present only in these situations.

Animals↗
PubMed1957

Fluorescent antibody detection of the antigens of the Shope papilloma virus in papillomas of the wild and domestic rabbit.

The results obtained by a fluorescent antibody study of the Shope papilloma virus in papillomas of the wild and domestic rabbit are presented. In the wild rabbit papillomas the viral antigens occurred exclusively in the nucleus and were present in the differentiating cells of the keratohyaline layers and in the keratinized layers. The antigens were not present in the deeper proliferating epithelial cells of the papillomas. The Shope viral antigens were present in very minute amounts in papillomas of the domestic rabbit, as compared with papillomas of the wild rabbit, and were only detected in the superficial keratinized layers. It is postulated that virus is present in the nuclei of the proliferating cells of the papillomas of the wild and domestic rabbit but exists there in an early stage of development, consisting mainly of nucleic acid and deficient in protein, therefore non-antigenic and not demonstrable by fluorescent antibody. The nucleic acid moiety of the virus may be infective, and the protein component may provide immunologic specificity and serve to preserve transmissibility. The protein-deficient virus can be referred to as masked virus.

Animals↗
PubMed1955

Studies on the increase of vaccine virus in cultured human cells by means of the fluorescent antibody technique.

The specific detection of the antigens of vaccine virus in cultured human cells by means of fluorescent antibody is reported. The antigenic material occurred in the cytoplasm in progressively increasing amounts and in the early stages was in close contact with the nuclear membrane. The antigens were present in a finely particulate form, either as foci or loosely spread out, up to late stages of infection, at which time the staining assumed a homogeneous form due either to close packing of the particles or to soluble antigen, or both. In late stages, homogeneous collections of antigen were observed in the nucleus. They may have resulted from diffusion of soluble antigen through the nuclear membrane. The antigens were observed in situations which suggest a new method of spread from one to another of these cells, namely by transmission through cytoplasmic connections.

Antibodies↗
PubMed1955

Visualization of Egypt 101 virus in the mouse's brain and in cultured human carcinoma cells by means of fluorescent antibody.

The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both cases the antigens occurred in the cytoplasm of the cells exclusively. In the early stages of infection the antigen was localized about the nucleus of the human cultured cells. In some cases the staining of the antigens produced a sponge-like effect, probably because of the numerous vacuoles of lipid material. The antigens were observed in the protoplasmic connections between cells and they probably served as a main route of spread of the agent in these human cell cultures. The number of cells initially infected and showing antigen was proportional to the amount of virus added, and titration with one substrain of cells proved much more sensitive than intracerebral titration in the mouse.

Animals↗