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Biomedical subjects

W F Zheng

Publications and source records attributed to W F Zheng.

12 recordsLinked to original sources
PubMed1999

Mono- and sesquiterpenes and antifungal constituents from Artemisia species.

In addition to beta-sitosterol and alpha-amyrin detected in all the investigated species, the extract of the aerial parts of Artemisia giraldii var. giraldii gave stigmasterol, daucosterol, sesamine, luteolin, eupafolin, hispidulin, eupatilin, belamcanidin, pinitol, artemin, ridentin, and a new antifungal monoterpene (named santolinylol) while that of the aerial parts of A. mongolica afforded sesamine, eupafolin, eupatilin, matricarin, and a new germacranolide (3-oxo-11 alpha H-germacra-1(10)E,4Z-dien-12,6 alpha-olide), and that of the aerial parts of A. vestita yielded stigmasterol, daucosterol, umbelliferone, scopolin, scoparone, and isoscopoletin-O-glucoside. Pinitol, first reisolated from Artemisia genus, was shown to inhibit the growth of the human pathogenic fungi Candida albicans, Aspergillus flavus, A. niger, Geotrichun candidum, Trichophyton rubrum, and Epidermophyton floccosum. Umbelliferone was also active against Candida tropicalis, A. flavus, G. candidum, T. rubrum, and E. floccosum. The flavones hispidulin and belamcanidin were almost equally inhibitory to the growth of A. flavus, G. candidum, T. rubrum, and E. floccosum, and santolinylol to C. albicans, A. flavus, A. niger, G. candidum, T. rubrum, and E. floccosum. In addition, ridentin was active against the growth of the plant pathogenic fungus Cladosporium cucumerinum.

Antifungal Agents↗
PubMed1998

Biologically active substances from the genus Artemisia.

Artemisia species, widespread in nature, are frequently utilized for the treatment of diseases such as malaria, hepatitis, cancer, inflammation, and infections by fungi, bacteria, and viruses. Furthermore, some Artemisia constituents were found to be potential insecticides and allelopathic chemicals. This genus is receiving growing attention presumably due to: (i) the diversified biology and chemistry of the constituents, (ii) the frequent application in traditional medical practice, and (iii) the rich source of the plant material. This review summarizes mainly the biological results obtained in the past decade. The significance and trends in this field are briefly discussed.

Animals↗
PubMed1996

Two flavones from Artemisia giraldii and their antimicrobial activity.

Two new flavones, 4',6,7-trihydroxy-3',5'-dimethoxy-flavone (2) and 5',5- dihydroxy-3',4',8-trimethoxyflavone (3) were isolated from Artemisia giraldii and their structures were identified by spectroscopic methods. These two new flavones showed antibiotic activity against Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Proteus sp. Aspergillus flavus, and Trichoderma viride.

Anti-Bacterial Agents↗
PubMed1993

CT detection of wrist bone erosion in rheumatoid arthritis.

A correlative radiographic and CT study of the wrists of 30 patients with rheumatoid arthritis (RA) was reported. The results showed that sensitivity of CT in detecting wrist erosion is superior to that of conventional radiograph. Accordingly, wrist CT scanning is useful for early diagnosis of RA.

Adult↗
PubMed1990

[Comparative study of TRBC receptor and E receptor on T lymphocytes of human and macaque].

In 1985, rosette formation of human and macaque pan-T lymphocytes with tree shrew red blood cells (TRBC) (TRBC rosette) was first found by Ben K et al, showing different physico-chemical properties from that of rosette formation with sheep red blood cells (E-rosette). In order to approach the correlation between TRBC receptor, E receptor (CD2) and other differentiation antigens (CDs) on T lymphocytes, rosette inhibition assay and antigenic modulation or co-modulation were performed with monoclonal antibodies (McAbs) to CDs, and the distribution of TRBC receptor in other peripheral immunocytes, cell lines was also examined. TRBC rosette appeared in 88.8% of E rosette positive peripheral blood lymphocytes (E(+)-PBL) and in 4.16% of E(-)-PBL. TRBC receptor was also found on all T cell lines tested (CEM, H33 HJ-JA 1, Jurkat, MLA-144, Molt-3, Molt-4, Molt-4 clone 8, PEER) and some myeloid lines (U 937 and HL 60), but not on human granulocytes, B cell lines (Daudi, Raji and Reh) and myeloid line K 562. The modulation or co-modulation of CD 3, TCR, CD 5, CD 6 and CD 7 with McAbs OKT 3, T 108 (F 1), T 136 (F 101-15), T 149 (M-T 604) and T 152 (7 G 5) did not affect TRBC rosette formation of PBL. TRBC rosette of human and rhesus monkey PBL was not inhibited by T 11.1 McAb OKT 11 (CD 2 McAb), in contrast human and rhesus monkey E rosette formations were obviously blocked at inhibition rates of 77.9% and 49.3%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

Measurement and clinical significance of thyroid microsomal and thyroglobulin antibodies by enzyme-linked immunosorbent assay.

Using the thyroid microsomal antigen(TMAg) prepared by affinity chromatography gel filtration method of sufficient purity, we measured the TM antibody (TMAb) level by an enzyme-linked immunosorbent assay (ELISA) in 103 normal persons and 183 patients with various thyroid disorders (Hashimoto's thyroiditis, Graves' disease, hypothyroidism, subacute thyroiditis, thyroid cancer, thyroid adenoma and simple goiter). The thyroglobulin antibody (TCAb). T3 and T4 were also measured at the same time. Based on the measurement of TMAb and TGAb of the thyroid diseases and analysis of their incidences and titer, our data strongly support that ELISA using purified TM and TG is a very useful and promising method for diagnosis and distinguishing autoimmune from non-autoimmune thyroid disease, and also can be employed in monitoring the development and studying the pathogenesis of the disease. We found that there is a negative correlation between TMAb titer and T3, T4 values (P less than 0.01) which has not been reported before in the literature. According to the result of the study, we suggest an immunological classification of thyroid diseases.

Adult↗
PubMed1988

Effect of sex hormones on NK and ADCC activity of mice.

The effect of pharmacologic doses of sex hormones on NK and ADCC activity against YAC-1 lymphoma and CRBC target cells was studied. Estradiol (E) and testosterone (T) administration for 2 weeks caused a substantial reduction of splenic NK activity in TA3 mice of either sex. In prolonging the treatment time, the intensity of suppression was gradually increased. Both E and T have apparently no inhibitory effect on ADCC activity of TA3 mice, although the ADCC activity slightly increased in the early stage of T treatment. The ADCC activity of T-treated male mice was slightly higher than that of E-treated males. Passive transfer of the splenic mononuclear cells and serum of treated mice does not affect the NK and ADCC activity of normal recipient mice. Addition of different concentrations of sex hormones into the culture medium or pre-treatment of effector cells for 12h failed to change the NK and ADCC activity of murine splenic cells.

Animals↗
PubMed1987

An affinity chromatography-gel filtration device for preparing thyroid microsomal antigen.

On the basis of conventional differential centrifugation for preparing crude thyroid microsomal antigen (TMAg), we have employed Sepharose 4B gel filtration and affinity chromatography separately to study the elution pattern in terms of absorbance and antigenic activity. The result indicates that thyroglobulin (TG) exists in two forms in crude TMAg, i.e., 'free TG' and 'membrane-bound TG'. TMAg is present in two forms in the eluate: (1) the TM fragment or TMAg polymer, which is produced at a higher rate and has greater antigenic activity, but which is less pure; (2) soluble TMAg, which is produced at a lower rate and has less antigenic activity, but which is more pure. We have developed an affinity chromatography-gel filtration (AC-GF) device which is a combination of affinity chromatography and a Sepharose 4B column. Sephadex G-50 is placed between the rubber stopper and Sepharose 4B in the GF column to ensure intactness of the entire system. With such a device, the AC removes the contaminated TG from TM homogenate, and allows the latter to pass directly from AC to GF for rechromatography. This device extracts the full advantages of both methods and each compensates for any deficiency of the other. Using this one-step procedure, one has the greatest chance of removing TG and obtaining TM fragments of TMAg polymers of higher antigenic activity, as well as separating small amounts of more purified soluble TMAg. Thus, the newly developed method meets the need of large quantities of TMAg for practical application, and at the same time the more purified preparations can be used for analytical purposes.

Autoantigens↗
PubMed1985

Measurement of antibody-dependent cell-mediated cytotoxicity by Hb-enzyme release assay (Hb-ERA).

A non-isotope method, hemoglobin-enzyme release assay (Hb-ERA), for measuring antibody-dependent cell-mediated cytotoxicity (ADCC) has been successfully developed. The assay is based on the fact that hemoglobin has peroxidase activity capable of catalyzing the oxidation of o-phenylenediamine to give coloration which can be measured by spectrophotometer. In comparison to the conventional 51Cr release assay, Hb-ERA is economic, sensitive, reproducible and does not have the shortcomings associated with manipulation of radioactive substances. It is applicable in clinical and research laboratories of any scale. The optimum conditions for the assay are as follows: an effector cell: target cell ratio of 10:1 and an incubation period of 14-16 h at 37 degrees C in 5% CO2. The splenic ADCC activity of mice was measured. The cytotoxic index of mice aged 10-14 weeks (39.71 +/- 11.19) was significantly higher than that of 20-24 week-old mice (18.42 +/- 10.31) (P less than 0.001). The ADCC activity is not influenced by sex. The merits and drawbacks of Hb-ERA are objectively evaluated.

Animals↗