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W G Glenn

Publications and source records attributed to W G Glenn.

18 recordsLinked to original sources
PubMed1967

Quantitative analyses of certain enteric bacteria and bacterial extracts: I. Standardization and sonic disruption of eight enteric bacterial species, each at five population levels.

Standardized individual preparations of five population levels of eight enteric organisms [Escherichia coli (O4:H3), E. coli (O111:B4:H12), Salmonella enteritidis, S. paratyphi B, S. typhimurium, Shigella boydii, S. dysenteriae, and S. sonnei) were prepared. Dry weights, calculated mean cell weight, and nitrogen content of bacterial suspensions before, and of supernatant fluids after, ultrasonic disruption are tabulated. Percentages of disruption, estimated from nitrogen concentration ratios of the suspensions and supernatant fluids, are given. These data are presented as guidelines for the preparation of bacterial extracts prior to precipitin analyses.

Journal Article↗
PubMed1967

Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts: II. Discrimination of Sonic Extracts by Interfacial Densitometry of Precipitin Systems.

Bacterial extracts prepared by ultrasonic disruption were reacted with both narrow- and broad-spectrum reference (homologous) and cross-reacting (heterologous) precipitins produced in rabbits. Quantitation of the reaction was obtained by densitometry of the antigen-antibody interface. Comparisons were made of sonic extracts from various starting populations all equated to the same nitrogen concentrations, and of various nitrogen levels derived from five bacterial population levels prepared separately. Sources of error are probed to show under what circumstances cross-reactions would be of greater magnitude than reference ones. The feasibility was shown of using quantitative densitometry of the interface combined with broadly reacting precipitins to identify bacteria on an intergeneric and interspecies scale. Problems associated with the use of absorbed or monospecific precipitins are explained.

Journal Article↗