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Biomedical subjects

W Gehring

Publications and source records attributed to W Gehring.

At least 55 records · Page 3Linked to original sources
PubMed1988

Secondary structure determination for the Antennapedia homeodomain by nuclear magnetic resonance and evidence for a helix-turn-helix motif.

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was studied in aqueous solution by nuclear magnetic resonance (NMR). Sequence-specific resonance assignments have been obtained for the complete polypeptide chain of 68 amino acid residues. The secondary structure determined from nuclear Overhauser effects (NOE) and information about slowly exchanging amide protons includes three helical segments consisting of the residues 10-21, 28-38 and 42-52, respectively. Combination of the presently available NMR data with computer modeling provided preliminary evidence for the presence of a helix-turn-helix motif in the homeodomain. Near the turn, this supersecondary structure appears to be very similar to the DNA binding site in the 434 and P22 c2 repressors, but both helices in the homeodomain include 2-3 additional residues when compared with these prokaryotic DNA-binding proteins.

Amino Acid Sequence↗
PubMed1988

[The modern, pharmacologically oriented prescription].

Making up a prescription is only expedient, when a pharmacologically reasoned therapeutical concept cannot be carried out with ready-made specialties. The possibilities of scientifically oriented external treatment are exemplarily demonstrated by treatment with antiinflammatory externals. We recommend the so-called step therapy, i.e. starting with a powerful corticoid, changing to a moderately strong corticoid after a few days, and finally, as soon as possible, using hydrocortisone only. At least from time to time, this treatment should be replaced by the application of Bufexamac. The concept of stepwise therapy largely reduces the hazard of loss in efficacy due to the tachyphylaxia phenomenon, as well as the risk of side effects. Since frequently allergological risk patients are concerned, treatment with the active substances mentioned must be possible using the same base in variable concentrations. In addition, the base must meet the requirements for hypoallergenicity. We make suggestions for a therapeutical concept of this kind based on the frequency of sensitizations, which occurred in the cases reported at the Dermatological Clinic in Karlsruhe (FRG).

Adrenal Cortex Hormones↗
PubMed1988

[IgM kappa immunocytoma in IgM-kappa monoclonal gammopathy].

A case of monoclonal gammopathy of the IgM kappa class is reported, in which a large number of reddish-brown, non-itching papules, reaching at maximum the size of peas and aligned in a follicular arrangement, had been seen to an unchanged extent on the left upper thigh for 6 years. Both histologically and immunohistologically, a B-cell lymphoma releasing kappa-type IgM was demonstrated. The clinical picture obtained was different from that of the few other cases reported in the literature, which have always involved either large-surface infiltrates or much larger nodes, either solitary or irregularly arranged.

Aged↗
PubMed1987

[Local treatment of mycosis fungoides with dichlorodiethyl sulfide in combination with phototherapy].

9 patients suffering from mycosis fungoides in various stages were topically treated with dichlorodiethyl sulfide solution associated with photochemotherapy. Topical application of dichlorodiethyl sulfide is indicated in the stages 1 to 3. In more advanced stages, the cutaneous lesions showed good response to therapy, but systemic lesions could not be influenced. Only one patient developed sensitization to topical dichlorodiethyl sulfide. The low incidence of allergic complications may be due to the combined therapy of dichlorodiethyl sulfide and photochemotherapy.

Aged↗
PubMed1982

Physiological studies of exocrine pancreatic secretion in conscious rats. 4. Communication. Effects of anesthesia on flow rate and composition of pancreatic juice.

1. In conscious rats provided with indwelling pancreatic and biliary catheters, the influence of anesthesia was investigated. 2. Ether was applied in concentrations of 4, 6 and 8 Vol % by an evaporator and Na-pentobarbital injected intravenously (30 mg/kg b. w.) 3. Flow rate, electrolytes (ion-selective electrodes and flame photometry), total protein concentration and the amount of several different individual enzyme fractions were determined quantitatively by electrophoresis. 4. During ether anesthesia flow rate and total protein concentration are decreased by about 50% of the control. A certain constant basal flow is established. Na+-Concentration is increased (+15%) and K+-concentration is diminished by about 30-35%. Characteristic changes in the enzyme spectrum are induced. 5. Changes due to pentobarbital anesthesia are as follows: reduction of the mean flow rate (30-35%) with large fluctuations. Corresponding variations of the K+-concentration. Diminution of the protein content without typical changes in the individual enzyme fractions.

Anesthesia, General↗
PubMed1981

A DNA segment isolated from chromosomal site 67B in D. melanogaster contains four closely linked heat-shock genes.

cDNA clones coding for two different small heat-shock polypeptides were isolated. Both clones hybridize exclusively to the heat-shock puff site 67B, and restriction mapping of embryonic Drosophila melanogaster DNA showed that the two genes probably occur as single copies and are closely linked. The analysis was extended by isolating genomic clones, which contain these genes and two additional ones. The four different genes code for heat-induced poly(A)+ RNAs. These genes are clustered within an 11 kb segment and are separated by spacers of 1.0-4.7 kb. Three of the genes were found to exhibit alternating polarities. Thus in spite of their close linkage, the four heat-induced genes are most likely organized in individual transcription units.

Chromosomes↗
PubMed1973

Isolation and characterization of mitochondrial DNA from Drosophila melanogaster.

Mitochondrial DNA (MtDNA) with a neutral buoyant density of 1.681 g/cm(3) has been isolated from unfertilized eggs of Drosophila melanogaster. This DNA is a circular molecule with an average length of 5.3 microm; it reassociates with a low C(0)t(1/2) after denaturation, and in alkaline isopycnic centrifugation it separates into strands differing in density by 0.005 g/cm(3). MtDNA isolated from purified mitochondria of unfertilized eggs or from total larval DNA melts with three distinct thermal transitions. The three melting temperature values suggest that the molecule may have three regions differing in average base composition. DNA isolated from unfertilized eggs of D. melanogaster contains approximately equal amounts of MtDNA and another DNA with a buoyant density of 1.697 g/cm(3), slightly less dense than main peak DNA. The possibility that the heavier DNA fraction consists of amplified ribosomal DNA was excluded by hybridization experiments, but otherwise nothing is known of its origin or function.

Animals↗
PubMed1972

Repair of the lethal developmental defect in deep orange embryos of Drosophila by injection of normal egg cytoplasm.

The eggs produced by deep orange females (dor/dor) of Drosophila melanogaster have a defect that causes early death of the deep orange embryos derived from these eggs. Ovaries transplanted from deep orange females to normal female hosts continue to produce defective eggs, showing that the dor mutation directly affects an ovarian function. In heterozygous embryos (dor(+)/dor) derived from eggs of deep orange females, the presence of a paternal dor(+) gene enables normal development to occur in about half of the embryos. Thus, the egg defect caused by the dor mutation is repairable after fertilization by the action of a dor(+) gene. The deep orange egg defect has also been repaired by injection of cytoplasm from unfertilized normal eggs into deep orange embryos at the syncitial preblastoderm stage of development. About one-third of the injected deep orange embryos were able to continue their development to an advanced stage of embryogenesis. This developmental response can serve as a bioassay for the identification of the defective component in eggs of deep orange females.

Animals↗