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Biomedical subjects

W Grüninger

Publications and source records attributed to W Grüninger.

10 recordsLinked to original sources
PubMed1992

[New aspects in the diagnosis of herpes simplex virus encephalitis--case reports and virologic findings].

Herpes simplex virus (HSV) is one of the most common causes of severe necrotizing encephalitis in men. Without adequate acyclovir treatment it has a mortality rate of 70%. An early, reliable virological diagnosis is therefore essential. In this study, two newly-introduced diagnostic methods were assessed and compared: Polymerase Chain Reaction (PCR) for detection of HSV-genome in cerebrospinal fluid and HSV-specific isoelectric focusing (IEF) for detection of intrathecal virus specific antibody synthesis in HSV encephalitis (HSVE). PCR proved more effective for the early diagnosis of HSVE and HSVE relapses, whereas HSV-specific IEF was superior for the diagnosis of HSVE in post-acute stages of the disease.

Adolescent↗
PubMed1977

Autoradiographic demonstration of proliferating cells in cerebrospinal fluid.

The proliferative activity of cells, isolated from 82 human CSF specimens, was examined by 3H-thymidine autoradiography. High labelling indices (LI) were found in acute viral meningitis (up to 8 per cent) and radiculitis (up to 6 per cent). CSF cell proliferation was also shown in the subacute stages of viral diseases and in other inflammatory processes (LI ranging from 0.5 per cent to 3 per cent). Most of the cells labelled from these CSF specimens were large lymphocytes, "lymphoid cells" and plasmacytes. Their presence in CSF is presumed to indicate an immune reaction. By the demonstration of a proliferative activity of these cells, aseptic inflammatory processes can be differentiated from "unspecific" pleocytosis. Because of a correlation between the LI of CSF cells and the stages of some inflammations, this method is suggested for an assessment of pregression or remission of chronic processes, e.g. "chronic meningitis" and multiple sclerosis. It can also be used in experimental research: the same type of mononuclear cells was labelled after having been cultured for 23 hours prior to the incubation with 3H-thymidine. Proliferating tumor cells as well as proliferating non-neoplastic mononuclear cells were demonstrated in CSF from various neoplastic diseases. In the clinical diagnosis of these processes, the method is of limited value. It proved very useful, however, for an assessment of the therapeutic effects of intrathecal cytostatic therapy. CSF specimens from non-inflammatory and non-neoplastic diseases regularly contained very few proliferating cells (LI: less than 0.1).

Autoradiography↗
PubMed1977

The effect of electrical stimulation on the experimentally denervated rat muscle.

The m. quadriceps of white rats was electrically stimulated after nerve crush and after nerve section. Electrically stimulated muscles showed fewer central nuclei and a greater number of necrotic single fibres. The demonstration of motor endplates could not give a reliable indication of the onset of reinnervation. The various results show the importance of the trophic influence of the nerve and raise the suspicion that electrical stimulation has a retarding effect on the atrophy but also on regeneration of the fibres. After 7 weeks the unstimulated muscles show a greater degree of regeneration than the stimulated ones.

Animals↗
PubMed1976

Intrathecal cytostatic chemotherapy of meningitis carcinomatosa. Clinical manifestation and cerebrospinal fluid cytology in a case of metastatic carcinoma of the breast.

Effects of intrathecal cytostatic chemotherapy on the clinical symptoms and cerebrospinal fluid (CSF) findings are being demonstrated in a case of metastatic carcinoma of the breast with sighns of meningeal irritation and cranial nerve involvement. Clinical symptoms disappeared after repeated intrathecal applications of methotrexate. CSF cell counts were reverted to normal but tumor cells were found to be persistant in CSF over a period of 19 months. Risks of combined intrathecal and systemic cytostatic therapy and possible effects of parenteral citrovorum factor applications are being discussed. The importance of continued intrathecal chemotherapy and of regular and thorough examination of CSF cells in meningitis carcinomatosa is emphasized. Cytological methods included investigations on viable CSF cells.

Adenocarcinoma, Mucinous↗
PubMed1976

[Possibilities of cervical fusion surgery in the spinal canal with intervertebral disk degeneration, Therapeutic and case-report contribution].

This is a report of a patient with pains in the shoulders and arms, atrophies of the small muscles of the hands, and paraspasms of the legs. After having suffered from the disease for eight to nine years, the patient was diagnosed as having cervical myelopathy. Measurements of the bony cervical vertebral column, which were made on a lateral roentgenogram, showed extreme narrowness of the spinal canal, the measured values obtained being below the lower limit. In addition, the cervical canal was narrowed at three levels by the formation of projections on degenerate disks. This suggested the most effective surgical treatment: Vetral clearance of disks, removal of prominences, and fusion of the three levels through the use of four bone dowels. Subsidence of pains in the shoulders and arms, improvements of paresis of the hands, and considerable improvement of paraspams of the legs were observed postoperatively. This particular case shows that surgical intervention may be considered a possible method of therapy even in the case of narrowness of the cervical canal, a condition that was aggravated here by a number of degenerate disks. In such situations it is possible for the cervical canal to be considerably relieved by clearing the disks, removing the projections and crests, and making use of the special technique of fusion or joining together so that improvement will be possible even in those cases where the disease has already arrived at a progressive state.

Cervical Vertebrae↗