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Biomedical subjects

W Gross

Publications and source records attributed to W Gross.

At least 19 recordsLinked to original sources
PubMed1992

Release of carrot plasma membrane-associated phosphatidylinositol kinase by phospholipase A2 and activation by a 70 kDa protein.

Plasma membranes were isolated from carrot (Daucus carota L.) cells grown in suspension culture and treated with phospholipase A2 from snake or bee venom for 10 min. As a result of this treatment, phosphatidylinositol kinase activity was recovered in the soluble fraction. There was no detectable diacylglycerol kinase or phosphatidylinositol monophosphate kinase activity released from the membranes after the phospholipase A2 treatment. Treating the plasma membranes with phospholipase C or D did not release PI kinase activity. The phospholipase A2-released PI kinase was activated over 2-fold by a heat stable, soluble 70 kDa protein. The partially purified 70 kDa activator increases the Vmax but does not affect the Km of the phospholipase A2-released PI kinase.

1-Phosphatidylinositol 4-Kinase↗
PubMed1979

[Hemorrhagic diathesis in patients with malignant neoplasms (author's transl)].

Clotting analysis in 30 patients with bleeding complications in malignant hematological diseases revealed the following troubles: The global tests, Quick's index and partial thromboplastin time markedly differed from normal. Activity of clotting factors revealed hypo- or hyperfibrinogenemia, disturbances of the prothrombin complex (factors II, VII, IX and X), decrease of factors V, VIII, XII. Factor XI (= PTA) was not diminished in any case. Regarding the fibrin-stabilizing factor (factor XIII), its activity was significantly decreased in 30 patients with solid tumors and in 30 patients with hemoblastoses. Faulty clotting balance was characterized by hyperfibrinolysis or disseminated intravascular coagulation (DIC) accompanied by reactive hyperfibrinolysis. About one quarter of the patients with malignant disturbances of the hematopoetic system demonstrated (mostly amegacaryocyte) thrombocytopenia. Finally, treatment of bleeding complications in malignant neoplastic diseases is pointed out.

Blood Coagulation Tests↗
PubMed1979

[Effects of propranolol and metoprolol on beta-adrenergic metabolic responses (author's transl)].

In ten healthy volunteers infusion of 7 micrograms/kg orciprenaline produced typical changes in carbohydrate and fat metabolism due to beta-adrenergic stimulation. Pretreatment with intravenously administered propranolol or metoprolol significantly inhibited orciprenaline-induced changes in systolic blood pressure, heart rate and plasma FFA, potassium, glucose and glycerol levels. Propranolol (0.1 mg/kg) seemed to be more effective than metoprolol (0.2 mg/kg) even in inhibiting predominantly beta 1-adrenergic responses.

Adult↗
PubMed1979

Metabolic changes after application of kallikrein in an ergometrical test.

Kallikrein given in a dose of 3 times 200 KE daily over 7 days caused under our test conditions an increase of ATP in blood and a decrease of lactate and especially of pyruvate. Glucose showed under this condition a higher utilization with a lowering of glucose in blood and an increase of the RQ. The acid-base metabolism follows these tendencies. In other experiments we did not find such effects, so the results must be specific to kallikrein.

Adenosine Triphosphate↗
PubMed1979

Long-term studies on therapy and tolerance of ketoprofen.

The therapeutic value of non-steroid anti-inflammatory agents for therapy of rheumatoid arthritis is discussed and a long-term study is described which was carried out for up to 12 monts on tolerance of ketoprofen compared with indomethacin. Objective laboratory data were used as criteria for possible influence on organ function. No abnormalities in renal and liver function were observed.

Arthritis↗
PubMed1979

[Urodynamic miction studies--physiological aspects].

In this work, definition and interpretation of different functional parameters, which were obtained through urodynamic investigations, are given. Normal patterns in child age are presented. They were the results of personal studies in patients with normal urinary tract. The pressure curve must be seen as an entity. The so-called after contraction is no detrusor contraction. But possibly it is the result of a determined lapse of contracting and relaxing occurrences in the different parts of the bladder and its outlet at the end of micturition.

Adolescent↗
PubMed1978

[Urodynamic data].

This paper gives a survey of urodynamic data that may be used for evaluating functional aspects of the bladder and its excretory tract. The different parameters are physically defined. Units of the International System are used in the dimensional formulae, which facilitates the establishment of relations between these parameters. The so-called urethral drag can be derived from the pressure law of Blasius. The calculation is as follows: urethral drag = vesical pressure/(maximum urethral discharge). This value is proportional to the true vesical resistance in a physical sense.

Humans↗
PubMed1978

[Method of measuring pressure using perfusion manometry].

Based on the principle of perfusion manometry, a device for the assessment of intraluminal pressures in urethra and anal canal is presented. The technical parameters are reported. The method may be utilized for clinical diagnosis in cases of disturbed micturition and defecation in children as well as for the function of esophagus.

Anal Canal↗
PubMed1977

Characterization of L-aspartate uptake by Streptomyces hydrogenans.

Multiple transport systems for L-aspartic acid exist in Steptomyces hydrogenans. The intracellular accumulation of L-aspartate against a concentration gradient was immediately inhibited by proton conductors, such as carbonyl cyanide p-trifluoromethoxyphenylhydrazone, 2,4-dinitrophenol or nigericin. Transport activity was gradually lost when inhibitors of protein synthesis were added. L-Aspartate transport had two pH optima at 6.5 and 4.5. At pH 6.5, two saturable transport components with different Km and Vmax values could be resolved by kinetic studies. A high-affinity system (system I) preferred the L-isomers of the anionic forms of aspartic and glutamic acid. At the same pH, a second, low-affinity system (system II) operated, which was presumably less specific than system I and also able to accept, at high concentrations, neutral amino acids. At pH 4.5, the Lineweaver-Burk plot revealed only a single catalytic component, with Km and Vmax values similar to those of system II. Again, in contrast to system I, this component showed high affinity for neutral amino acids. The data suggest that L-aspartic acid and L-glutamic acid are transported by this system as neutral zwitterionic molecules.

Amino Acids↗