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W H GAYLORD

Publications and source records attributed to W H GAYLORD.

12 recordsLinked to original sources
PubMed1961

The vacuolating virus of monkeys. I. Isolation, growth characteristics, and inclusion body formation.

A vacuolating virus isolated from uninoculated patas monkey kidney cultures was found to be serologically identical with SV(40), a virus previously found in association with rhesus and cynomolgus monkeys. Detection of the patas virus was facilitated when patas cells were exposed to x-ray treatment. Rhesus monkey and human cells were relatively insusceptible to the virus, although it persisted in these cells for a long period of time. Distinct intranuclear inclusions were detected in infected patas cultures 2 to 3 days before cytoplasmic vacuoles were noticeable. Cultures previously infected with PA-57 virus did not affect yields of poliovirus, and doubly infected cells were easily distinguished in stained preparations.

Animals↗
PubMed1961

The vacuolating virus of monkeys. II. Virus morphology and intranuclear distribution with some histochemical observations.

Cells infected with the vacuolating virus, SV(40), respond by swelling to several times their normal volume. Within enlarged nuclei, virus-containing inclusions appear which are acidophilic and Feulgen-positive. The formation of nuclear inclusions is followed by the appearance of cytoplasmic vacuoles and then shrinkage of the cell. Inclusions were found to exhibit unique double staining when a light-green counterstain was used in the Feulgen reaction. The virus is of low electron density, round, and 300 A in diameter. It occurs in large numbers, singly and in short chains, and it appears to multiply at the expense of chromatin.

Animals↗
PubMed1954

Intracellular forms of meningopneumonitis virus.

Electron micrographs of intracellular memngopneumonitis virus have shown several types of particles which are presumably representative of different stages of a life cycle. They are: (a) Elementary bodies-dense particles 250 to 300 mmicro in diameter with very dense central granules. (b) Intermediate forms-less dense than elementary bodies and larger. They are 300 to 400 mmicro in diameter, contain a very dense central granule, and often have two or three limiting zones, (c) Circles 400 to 500 mmicro in diameter-homogeneous structures with single membranes and no internal granules. They are often elongated and constricted at the center in the manner of budding yeast cells, (d) Circles 500 to 600 mmicro in diameter with single membranes.mdash;One, two, and three dense granules may be present in some of these structures, (e) "Larger" structures, circular to elliptical, often with discontinuities or ruptures in their membranes. They sometimes seem to have internal septa. It has been inferred from these observations that the virus can multiply by binary fission or by multiple endosporulation and that elementary bodies are a spore-like stage.

Cell Division↗
PubMed1953

Intracellular forms of pox viruses as shown by the electron microscope (Vaccinia, Ectromelia, Molluscum Contagiosum).

The intracellular development of three pox viruses has been studied with the electron microscope using thin sections of infected tissue. Cells infected with vaccinia, ectromelia, and molluscum contagiosum viruses all form developmental bodies preliminary to the production of mature virus. Developmental bodies, believed to be virus precursors, are round to oval, slightly larger than mature virus particles, less dense to electrons, and have a more varied morphology. It is suggested as a working hypothesis that the process of maturation of a virus particle takes place as follows. In the earliest form the developmental bodies appear as hollow spheres, imbedded in a very dense cytoplasmic mass constituting an inclusion body, or in a less dense matrix near the nucleus in cells without typical inclusion bodies. The spheres become filled with a homogeneous material of low electron density. A small, dense granule appears in each developmental body and grows in size at the expense of the low density material. Following growth of the granule, particles are found with the dimensions of mature virus and having complex internal structure resembling bars or dumbells. Mature virus is ovoid and very dense to electrons. An "empty" interior may be found within its thick walls.

Cytoplasm↗