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Biomedical subjects

W Hackl

Publications and source records attributed to W Hackl.

At least 73 records · Page 4Linked to original sources
PubMed1988

Immunoelectron microscopic localisation of ribosomal proteins from Bacillus stearothermophilus that are homologous to Escherichia coli L1, L6, L23 and L29.

The locations of proteins BL1, BL6, BL23 and BL29 from Bacillus stearothermophilus have been determined on the ribosomal surface by immunoelectron microscopy. All four proteins were localized in the same region of the 50S subunit as their homologous counterparts from Escherichia coli, indicating that the ribosomal architecture is the same in both species. This finding is of great importance as it allows structural data obtained on ribosomes from either organism to be incorporated into a unique ribosome model.

Antibodies, Bacterial↗
PubMed1988

[Protracted action of vecuronium in a patient with chronic renal insufficiency treated by dialysis].

Although the pharmacokinetics of vecuronium are altered by the loss of kidney function, they do not differ significantly between patients with normal renal function and patients with renal failure. Therefore, the drug has become a preferred neuromuscular blocking agent in anuric patients. The author observed complete relaxation--verified by nerve stimulation--for more than 3 h following a single dose of 0.09 mg/kg vecuronium in a patient with chronic renal failure. Liver function was normal, and no drugs known to interact with vecuronium were used. The authors conclude that the altered pharmacokinetics of vecuronium in anuric patients might cause clinically significant effects in some patients.

Adult↗
PubMed1988

[Malignant hyperthermia in Austria. III. Anesthesia in susceptible patients].

Anesthesia in patients susceptible to malignant hyperthermia (MH) is generally considered to be very risky, although - with one notable exception - there are no prospective studies about anesthetic management in a large number of such patients. The prophylactic use of dantrolene has been recommended in MH patients, although there is no strong evidence supporting this - despite the fact that dantrolene may have serious side effects. We therefore decided to report the results of our own anesthetic technique for MH patients, as our technique does not include the prophylactic use of dantrolene. From 1981 to 1988, 19 operations on 16 MH-susceptible patients were performed. Patients 1-4 were pediatric survivors of an MH episode, where MH susceptibility was confirmed by muscle biopsy and in vitro contracture tests in at least one parent; patients 5-7 were survivors of an MH crisis, and they later underwent diagnostic muscle biopsies themselves; all other patients (nos. 8-16) were relatives of MH survivors with positive in vitro contracture tests. Diazepam, pentobarbital, pethidine, and chlorprothixene were used for premedication; no prophylactic dantrolene was given. Anesthesia was induced by thiopentone and was continued by nitrous oxide/oxygen, fentanyl, and droperidol; alcuronium, atracurium, and vecuronium were administered as necessary. Pyridostigmine, atropine, and naloxone were used if appropriate. New or disposable tubings were used for ventilation, and the vaporizers were removed from the anesthesia machines. ECG and body temperature were recorded in all patients; blood pressure was monitored invasively if indicated; end tidal CO2 was monitored whenever possible.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1988

[Malignant hyperthermia in Austria. II. A comparison of the results of diagnostic test procedures].

During the last 4 years different diagnostic procedures for the detection of malignant hyperthermia (MH) susceptibility have been used at the authors' clinical unit; this study was designed to compare the results of these tests. PATIENTS AND METHODS. Since March 1983, 158 patients have been referred for the following reasons: group A: probands (n = 17) who had had symptoms of MH during anesthesia; group B: patients of probands (n = 48) if the latter were not tested because of age (n = 24) or death (n = 2); group C: relatives from MH families (n = 86); group D: patients (n = 5) who developed fever during stress and/or physical activity (n = 3), had myotonia (n = 1), or developed rhabdomyolysis during intensive care (n = 1); group E: controls (n = 2). Two static halothane and two static caffeine tests according to the European protocol were performed in all patients (n = 158). Histological examinations of skeletal muscle (fixed in glutaraldehyde, stained with hematoxylin-eosin, Gieson, and toluidine blue) were done in the first 100 patients; all specimens were scored by the same investigator (E.S.). Score 0: normal; 1: increased number of sarcolemma cores; 2: 1+cores forming groups; 3: 1+2+fiber degeneration; 4: specific changes-myopathies. Plasma levels of creatine kinase (CK) were determined in the first 50 patients. Complete neurological examinations, including electromyography (EMG), were done in ten patients who had increased CK levels as well as histological scores of 3 or 4 (Table 1).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

[Effect of molsidomine on hemodynamics in patients with dilated cardiomyopathy].

The effect of 4 mg Molsidomine iv. followed by a continuous infusion of 3 mg per hour for 3 hours on arterial blood pressure, pulmonary artery pressure, cardiac index, peripheral and pulmonary vascular resistance and heart rate was evaluated in eleven patients suffering from chronic heart failure caused by non-ischaemic dilatative cardiomyopathy (NYHA II-III). A significant decrease in systemic blood pressure, systolic and mean pulmonary artery pressure and pulmonary wedge pressure was observed and also a marked decrease in total peripheral and pulmonary vascular resistance. There was a slight, but not significant increase in cardiac index. The heart rate did not change significantly. Treatment had to be stopped in one patient because of side effects (hypotension, nausea).

Adult↗
PubMed1986

[Comparison of fentanyl and tramadol in pain therapy with an on-demand analgesia computer in the early postoperative phase].

17 patients undergoing cholecystectomy in non-opiate general anaesthesia received tramadol (n = 7) or fentanyl (n = 10) for immediate postoperative pain relief using the on-demand analgesia computer (ODAC). Heart rate, blood pressure, and respiratory rate were monitored at half-hourly intervals during the 6-h trial period. Arterial blood was withdrawn at hourly intervals for blood gas analyses and beta-endorphin plasma level assays. Fentanyl and tramadol serum levels were determined prior to each on-demand bolus injection during the first 2 h of the study. At the end of the trial period, the quality of analgesia was assessed retrospectively using a visual analog scale. Mean opiate consumption was 0.53 +/- 0.1 mg for fentanyl and 412 +/- 11.6 mg for tramadol, resulting in an equipotency ratio of about 1:980 (relating to body wt., consumption/h, and pain score). No correlation was found between body wt.-based opiate requirements and pain score. Heart rate increased slightly but significantly under both opiates. Fentanyl produced a significant drop in mean arterial pressure by a maximum of 16%, while tramadol left mean arterial pressure unchanged. Respiratory rate, which was elevated initially, dropped significantly in both groups. Arterial pO2 and pCO2 were within the normal range throughout the observation period, reflecting the absence of respiratory side effects. Opiate blood levels showed major inter- and intraindividual variations (minimal and maximal levels for fentanyl ranged from 0.44-3.44 ng/ml, for tramadol from 272-1,900 ng/ml) and were thus poor predictors of the quality of analgesia.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1980

[Investigations of the lysine requirement of fattening boars in dependence on their performance. 3. Influence of energy consumption on the fat and protein retention of the boars].

The results of the carcass analysis show a distinct influence of the duration of keeping on protein retention. The protein percentage was highest with those animals whose lysine requirement was met 100%. A lysine supplement of 20% of the valid norm had no infuence on the percental protein content. In both cases (100% and 120%) 17.1% protein were determined in the carcass. Concerning the daily protein retention, boars whose protein requirement wes met 120% and with a live weight of more than 90 kg achieved more favourable results than those of the norm variant. Up to 90 kg live weight no difference between the two variants could be detected. Animals which received feed rich in energy showed a very high daily protein retention during the first third of the fattening period, which decreased relatively much, however, in the course of growth.

Animals↗
PubMed1979

[Performance dependence of the lysine requirement of fattening sows. 1. Effect of energy and amino acid consumption on fattening performance].

Seven variants with graduated energy and amino acid levels were tested at 105 growing sows in a live weight range between 30 and 120 kg. The weight increase and the energy expenditure showed a distinct dependence on the energy level of the ration and are mainly determined by the energy intake. Up to a live weight of 90 kg a 20% lysine over- or undersupply in comparison with the norm had no influence on the growth intensity of the animals. On the low and medium energy levels there was a tendency of superiority of the animals which received feeds rich in protein in the section between 90 and 120 kg. In the variant with a high energy level, however, a diminishing increase was detected in the last third of the fattening period in connection with the growing lysine level. The overall most suitable result was achieved in variant M 120 with a daily increase of 675 g and an energy expenditure of 2.04 kilo energetic feed units for pigs per kg increase.

Amino Acids↗
PubMed1979

[Lysine requirement of fattening boars in respect to their performance. 1. Influence of the consumption of energy and amino acids on the fattening performance].

With a total number of 157 boars 9 feeding variants with different levels of energy and amino acids were checked. The weight increase per fattening day and the energy and lysine expenditure per kg live weight increase were determined. The insufficient supply with lysine had negative effects on the live weight increase and the energy expenditure during the whole fattening period. Between the variantes with a high supply with amino acids (120%) and the supply according to standard (100%) no significant differences concerning the live weight increase could be ascertained up to 90 kg live weight. Between 90 and 120 kg live weight the animals in the variant with medium energy level and 120% lysine supply achieved significantly higher increases than those in the standard variant. Up to 90 kg live weight the energy expenditure for all groups amounted to 2,08 kEFs (energetic feed units for pigs) per kg live weight increase. The most suitable results were achieved by the animals which received the variants rich in energy (1,760 resp. 1,770 kEFs per kg live weight increase). Above the 90 kg live weight limit the energy expenditure rose considerably, remained however under 2,000 kEFs per kg live weight increase for the boars of the variant with medium energy level and 120% lysine supply as well as for the variant rich in energy and 100% lysine supply. The lysine expenditure in the first variant amounted to 25.1 g per kg live weight increase and for the variant rich in energy to 20.6 g lysine per kEFs.

Amino Acids↗
PubMed1979

[Lysine requirement of fattening boars in respect to their performance. 2. Relations between energy consumption and live weight increase].

Linear relations between live weight increase (y in g per kg live weight 0,75) and the daily energy consumption (x in energetic feed units pigs per kg live weight 0,75) were established. y = 0.8308 x-18.58, r = 0.7944 Extrapolation concerning the live weight balance showed an energy maintenance requirement of 22,36 energetic feed units for pigs resp. 78.26 kcal net energy fat for pigs per kg metabolic body weight. 121 energetic feed units for pigs resp. 424 kcal net energy fat for pigs were required for 100 g live weight increase.

Animals↗
PubMed1978

[The performance-related lysine requirement of fattening pigs. 2. Relationship between energy consumption and live weight gain].

With growing pigs (barrows) in the 40 to 120 kg live weight range, a narrow linear relationship of y = 21.06 + 0.7935x(R = 0.9599 sb = 0.0315) was established between daily live weight gain (y in g/kg 0.75) and daily energy consumption (x in feed equivalents (swine)/kg 0.75). The interpolation of the liveweight equilibrium produced a maintenance requirement of 26.5 EF(s) or 93 kcal NEF(s) per kg 0.75. 126 EF(s) or 441 kcal NEF(s) were needed for every 100 g extra live weight gain. The relationships found between energy consumption and gain agree very well with the NEF(s) requirement norm of growing pigs as derived by SCHIEMANN.

Animals↗
PubMed1978

[Performance dependent lysine requirement of fattening pigs. 3. Effect of amino acid and energy intake on fat, protein and lysine deposition of swine].

The gain in structural matter had a linear course with a corresponding feeding intensity and depending on the period of fattening. However, the animals given high-energy diets deposited 130 g protein per day during the first fattening period, this deposition being compensated in the course of further growth by a considerably lower deposition in the sense of aequifinality. Up to a live weight of 70 kg, animals subjected first to restricted feeding and then to fully balanced feeding revealed the highest protein deposition during the last period of fattening, this fact emphasizing the leanmeat character of the animal material used. The daily fat deposition was found clearly determined by energy intake and independent of amino acid supply. Lysine conversion was influenced by the intake of lysine and energy. Under feeding to norm it reached some 40 and 30% during the first and second periods of fattening, respectively.

Adipose Tissue↗
PubMed1978

[Studies on the performance-related lysine requirement in fattening pigs. 1. Influence of energy and aminoacid intake on fattening].

A total of 110 barrows fed varying levels of energy and amino acids in 8 treatments, were tested. The weight gains per fattening day and energy expenditure were found to be mainly determined by energy intake. Up to a liveweight of 100 kg both low and high levels of amino acid supply produced the same fattening performance results as amino acid supply to standard. In the 100-120 kg liveweight range, the animals fed higher levels of protein proved slightly superior. As to energy expenditure, animals subjected to restricted feeding up to 70 kg liveweight and fed full rations later, consumed 2.22 kEFs (2.22 kilo energetic feed equivalents/swine) per kg gain and proved significantly superior to those animals that were very intensively fed at the beginning and put to restricted feeding beyond 70 kg liveweight (2.53 kEFs per kg gain) as well as to those receiving high levels of energy all the time (2.39 kEFs/kg gain). In the latter case, 23 g of lysine were required per kg gain.

Amino Acids↗
PubMed1978

[Performance-related lysine requirement of fattening pigs. 4. Effect of energy and amino acid intakes on composition of gain and derivation of lysine requirement].

The results show that protein and fat syntheses proceed simultaneously in growing swine. Reducing the energy intake during the 2nd half of the fattening period in pigs of modern genetic construction (hybrid pig) is likely to cut both fat and protein deposition per day. This will result in lower daily gains, a longer fattening period and higher energy expenditure. Therfore, it is not advisable to restrict energy supply during the 2nd half of fattening of pigs derived from modern breeding lines. The optimum amino acid : energy ratio allows to make full use of the protein deposition capacity in pigs with prefixed slaughter weight. For the limiting amino acid lysine it was found to be 10.5 g/1,000 energetic feed equivalents (swine) or 3 g/Mcal NEF (swine), and, taking into account amino acid digestibility, 9.5 g dig. lysin/1,000 energetic feed equivalents (swine) and 2.7 g dig. lysin/Mcal NEF (swine), respectively.

Animals↗
PubMed1976

[Studies on the true amino acid digestibility in pigs of various wheat varieties from the Soviet Union].

Studies were carried out with pigs for determining, with classical methods, the true digestibility of amino acids contained in 5 intensively grown wheat varieties imported from the Soviet Union and in one wheat variety grown in the GDR. Of all the varieties tested the variety Mironovskaya 808 exhibited the best characteristics. An extremely low true lysine digestibility was established for the variety Avrora (68%). If this variety is to be more extensively grown and used for feeding purposes further studies will have to be made to see whether this low lysine digestibility is really characteristic of this variety.

Amino Acids↗