Biomedical subjects
Publications and source records attributed to W Hansen.
An easy to perform, rapid and precise assay for invertase is introduced. In it enzyme activity is determined spectrophotometrically from a coupled enzymatic reaction.
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A strain of vibrio cholerae Heiberg type II, not agglutinable with any of the eight antisera corresponding to Heiberg's groups, and a nonmotile, methyl red-positivs of chronic external otitis.
A halophilic Vibrio species was isolated from blood cultures from a 59-year-old male with enteritis. The strain differed from Vibrio parahaemolyticus and Vibrio alginolyticus by its ability to ferment lactose, its production of beta-galactosidase, and its lower NaCl tolerance. A report of this infection and a description of the isolate is presented.
A total of 314 strains of Haemophilus, isolated from clinical samples, were studied for the production of beta-galactosidase and beta-xylosidase. None of the H. influenzae strains studied (9 beta-lactamase positive strains and 129 beta-lactamase negative strains) possessed these enzymes. Both enzymes were almost constantly observed among strains of H. paraphrophilus (10 strains studied) and of H. paraphrohaemolyticus (9 strains studied). Among the other species (H. parainfluenzae, 55 strains; H. haemolyticus, 5 strains; H. parahaemolyticus, 97 strains), beta-galactosidase was present in about 30% of the strains studied whereas beta-xylosidase was detected occasionally (3% of the strains studied). Detection of these two enzymes could be a valuable test for the taxonomic study of the genus Haemophilus. However, the type of substrate used for the detection of beta-xylosidase is important: use of the para-nitro-phenyl-beta-xylopyranoside yielded more positive results than the use of its ortho-isomer.
Three hundred and five strains of Haemophilus (129 H. influenzae, 55 H. parainfluenzae, 97 H. parahaemolyticus, 5 H. haemolyticus, 10 H. paraphrophilus and 9 H. paraphrohaemolyticus) isolated from pathological material over the year 1976, were systematically tested for beta-lactamase production. Only 2 strains of H. parainfluenzae produced this enzyme. Both were able to transfer ampicillin resistance to Escherichia coli K12. All strains but the two beta-lactamase producers were susceptible to penicillin G, ampicillin and cephalotin. However, the correct interpretation of the susceptibility tests needed the microscopic observation of prints of the inhibition zones surrounding the disks: all sensitive strains presented a hazy growth around the disks which corresponded to the presence of spheroplasts; this phenomenon was not observed with the 2 beta-lactamase producing strains of H. parainfluenzae which grew up to the disks but presented a typical bacillary form.
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Over a period of 10 months hemodialyses in 20 patients with chronic renal failure were performed with a 40% lower heparin-dose. Blood coagulation was controlled from the activated partial thromboplastin time (APTT). There were no unfavourable side-effects, also the effectivity of the hemodialyses was not changed. However, we measured a significant increase in the hemoglobin-concentration and in the packed cell volume. Bleeding from the punctures was significantly shortened.
Monoglyceride hydrolase can be found in blood bound to thrombocytes or as free enzyme after the injection of heparin. Measurement can be performed easily with a new photometric method. The post-heparin activity is decreased in liver disease. This is probably due to the fact, that monoglyceride hydrolase is liberated from the liver's vessels.
21 patients with primary hyperlipoproteinemias of type IIb and IV were treated for 8 weeks with placebo, 8 weeks with 0.3 g butylbiguanide and 1.5 g clofibrate/day and then for 8 weeks with 1.5 g clofibrate/day. In 12 patients a second placebo phase of 8 weeks followed. After 8 weeks of combined treatment with butylbiguanide and clofibrate the serum triglycerides decreased from 725 mg to 269 mg/100 ml. During the following period of clofibrate treatment the serum triglycerides increased after 4 and 8 weeks to 326 mg and 306 mg/100 ml respectively. The combined treatment with 0.3 g butylbiguanide and 1.5 g clofibrate/day is more effective in lowering elevated serum triglycerides and cholesterol than 1.5 g clofibrate alone.
In the course of a systematic search for Bacteroides corrodents and Eikenella corrodens in clinical specimens submitted for microbiological analysis, 61% of the specimens from anal abscesses, 6% of the vaginal specimens and none of the pharyngeal specimens yielded B. corrodens, whereas E. corrodens was recovered from only 9% of the pharyngeal specimens. Some characteristics were found to be useful in differentiating between the two species: B. corrodens strains were strictly anaerobic, cytochrome-oxidase-negative, urease-positive and gelatinase-positive; they were sensitive to lincomycin but resistant to vancomycin. E. corrodens strains on the other hand were facultatively anaerobic, oxidase-positive, urease-negative and gelatinase-negative; they were resistant to lincomycin but sensitive to vancomycin. The pathogenicity of the two species was difficult to assess as in most cases they were recovered from mixed cultures.
One hundred and twenty-two clinical isolates of Acinetobacter were studied for the presence of beta-galactosidase and of beta-xylosidase, for biochemical characteristics, and for genetic interspecies transformation tests. All strains lacked beta-galactosidase; in contrast, beta-xylosidase was always present in the oxidative strains. This test proved to be of value for separating strains able to form acid from carbohydrates (A. anitratum and A. haemolyticus spp haemolyticus) from the non-oxidative strains (A. lwoffi and A. haemolyticus spp alcaligenes). However, the genetic relationship of all strains tested warrants further study before Acinetobacters are grouped into clearly defined species.
By means of a simple heparin test which reflects the actual processes in the contact of the blood with heterogeneous surfaces we can securely regulate the heparinisation in each phase of the dialysis, which, as I tried to explain, brings considerable consequences for the clinic.
The clinical course of arteriosclerosis was evaluated in 9 patients with coronary sclerosis and in 25 with peripheral arterial occlusive disease by means of repeat angiographies following primary angiography after 22.5 and 36.6 months, respectively. During the period of observation arteriosclerosis had progressed in all cases except in two patients with peripheral arterial occlusive disease. Despite intensive therapeutic efforts including control of risk factors, dietary care, guided or unguided exercise and the attempt to change unsatisfactory living conditions, progression of arteriosclerosis could not be interrupted. If treatment delayed progression of arteriosclerosis cannot be decided. All vascular areas were affected indiscriminately by progression and no particular localisation was preferred. Intensive exercise does not seem to delay progression of arteriosclerosis.
The activity of monoglyceride lipase was studied in plasma from 74 patients with liver disease in whom blood was drawn 10 min after the injection of heparin (10 U/kg body weight). The results were compared with the activities from 29 healthy volunteers. For measurement a new, easy to handle method was used. The patients showed significantly lower activities, e.g. in chronic aggressive hepatitis there was a decrease by 87%. There was no correlation between the activities of monoglyceride lipase and pseudocholinesterase which also can be decreased in liver disease. The results show that the determination of monoglyceride lipase appears to be a useful test of the liver's function. Its value probably lies in the evaluation of the vessels.
A monoglyceride hydrolase assay is introduced in which the activity of the enzyme is determined by the use of coupled enzymatic reactions. Measurements are easy to perform on a conventional photometer; they are specific, precise and show good reproducibility.