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Biomedical subjects

W Hryniewicz

Publications and source records attributed to W Hryniewicz.

104 records · Page 6Linked to original sources
PubMed1977

Effect of streptolysin S on human and mouse T and B lymphocytes.

The effect of streptolysin S on T and B human and mouse lymphocytes was studied. The dose-dependent inhibition of T cell rosette formation was observed, and this was accompanied by a dose-dependent cytotoxicity. The existence of a difference in the degree of susceptibility of T and B cells to the cytotoxic effect of streptolysin S is shown.

Animals↗
PubMed1977

Hemolysis induced by streptolysin S: kinetics of hemoglobin and 86Rubidium release.

Hemolysis of human erythrocytes produced by streptolysin S (SLS) was investigated. Kinetic studies of hemoglobin (Hb) release exhibited typical for SLS latent phase with hemolysis 30-60 minutes after addition of the toxin. Hb release was preceded by efflux of 86Rubidium (86Rb) which started at 5th-15th minutes after addition of the toxin. In erythrocytes treated with 2 HU/ml of SLS about 100% of 86Rb was released after 15 minutes; no hemolysis was observed at this time. Incubation of blood cells in 0.3 M sucrose or 6% DMSO prevented SLS induced hemolysis. Trypan blue was also inhibitory. Bovine serum albumin acclerated 86Rb and Hb release. The possible mechanism of this phenomenon is discussed. The results obtained indicate that SLS-produced hemolysis of human erythrocytes is an osmotic process.

Dimethyl Sulfoxide↗
PubMed1976

Bacteriocin production by group a streptococcal L-forms.

L-forms induced from a bacteriocin-producing strain of group A streptococcus retained both the ability to produce the streptococcin and producer strain immunity to the homologous bacteriocin. L-forms of a spontaneously cured (bacteriocin negative) derivative of this same strain failed to produce streptococcin but were sensitive to its action.

Bacteriocins↗
PubMed1976

Comparison of three methods for grouping streptococci.

Two new methods for serological grouping of beta-hemolytic streptococci, the nitrous acid extraction procedure of El Kholy et al. and the slide agglutination method of Christensen et al., were compared with the Lancefield hot-hydrochloric acid extraction method in classifying 92 strains of groups A, B, C, and G. The nitrous acid extraction method was easily performed, specific, and sensitive when highly potent antisera were used. For the Christensen method these highly potent antisera had to be diluted to avoid cross-reactions between groups A and C and groups B and G, respectively. A few strains, most of them group B, could not be grouped by the latter method. Using these three grouping methods, two sets of commercial sera were compared with the more potent sera supplied by R. C. Lancefield. The low antibody content of these commercial sera, especially anti-group B and G sera, contributed to the inferior results obtained in some of the grouping reactions.

Agglutination Tests↗
PubMed1976

Susceptibility of Staphylococcus aureus, Streptococcus pyogenes and Diplococcus pneumoniae to antibiotics. Comparison of patterns in Poland and Federal Republic of Germany.

One hundred and eighty five strains of Gram-positive cocci were tested for susceptibility to 16 antibiotics, including some newer drugs, by agar dilution technique. Of these, 100 strains (50 each isolated in Poland and Germany) were Staphylococcus aureus, 55 Streptococcus pyogenes, and 30 Diplococcus pneumoniae. Previous investigations of streptococci and pneumococci isolated in Poland and Federal Republic of Grmany, revealed no differences in antibiotic-susceptibility patterns in both countries, which was not the case with staphylococci. Present study demonstrates further evidence for existence of differences in antibiotic-sensitivity of staphylococci, depending of their geographical origin, and presents actual state of susceptibility to antibiotics of Streptococcus pyogenes and Diplococcus pneumoniae.

Anti-Bacterial Agents↗
PubMed1976

Susceptibility of Streptococcus faecalis to 32 antimicrobial agents. Comparison of patterns in Poland and Federal Republic of Germany.

Two hundred and twenty strains of Streptococcus faecalis (103 isolated in Poland and 117 in Federal Republic of Germany), were tested for antibiotic-susceptibility to 32 antimicrobial agents by agar dilution technique. Most effective in vitro appeared ampicillin, penicillin G, and a combination (1:1) of penicillin and streptomycin. Partly effective were also macrolide antibiotics, trimethoprim + sulfamethoxazole and five tetracyclines. Other antibiotics, including five cephalosporin derivatives, five aminoglycoside antibiotics, two lincomycins, two polymyxins, chloramphenicol and fucidin, appeared practically ineffective in vitro. Effectiveness of antimicrobials in vitro was considered on the basis of standard bioavailability of antimicrobial agents. No differences in antibiotic-susceptibility patterns of any practical significance were found, when strains isolated in Poland and Federal Republic of Germany were compared.

Anti-Bacterial Agents↗
PubMed1976

Susceptibility of clinically important gram-negative bacilli to 15 antibiotics. Comparison of patterns in Poland and Federal Republic of Germany.

Three hundred and sixty strains of Gram-negative bacilli (Escherichia coli, Salmonella enteritidis, Enterobacter cloacae, Providencia, indole-positive and indole-negative Proteus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Serratia marcescens), were isolated by random in Poland and Germany, and tested by agar dilution method for susceptibility to 15 antimicrobial agents, including six cephalosporin derivatives, colistin, gentamycin and tobramycin, three newer tetracyclines, ampicillin and carbenicillin, and a combination of trimethoprim and sulfamethoxazole. This investigation revealed existence of growing antibiotic-resistance of Gram-negative bacilli to all antimicrobial agents. A detailed analysis of this problem is described and a list of agents effective in vitro is presented. Some evidence has been found concerning possible existence of geographical differences in antibiotic-susceptibility patterns of strains of Gram-negative bacilli, isolated in Poland and Federal Republic of Germany.

Anti-Bacterial Agents↗