PubMed Health⌕ Search

Biomedical subjects

W Hubert

Publications and source records attributed to W Hubert.

14 recordsLinked to original sources
PubMed1993

Film-induced amusement changes in saliva cortisol levels.

The present study, aimed at investigating effects of film-induced amusement on saliva cortisol levels, was performed on 52 healthy male volunteers, aged 19-31 years. They were assigned to an amusement or a control condition. Saliva cortisol and affective film ratings were the dependent variables. During amusement 50% of the subjects showed changes in saliva cortisol correlated positively with ratings of funniness. Thus, cortisol secretion may be linked to emotional arousal, regardless of the emotional valence.

Adult↗
PubMed1992

In vivo calibration of microdialysis probes for exogenous compounds.

Several approaches for calibrating microdialysis probes for exogenous compounds in vivo are described which avoid the error introduced by in vitro calibration. These methods are based on establishing a steady state of the exogenous compound by a continuous (zero-order) iv infusion. The steady-state concentration is estimated by three methods that directly determine the in vivo concentration. The methods are (a) extrapolation of dialysate concentrations at various flow rates to the concentration at zero flow, (b) dialysis with concentrations of analyte added to the perfusion medium above and below the expected concentration to determine the concentration at no net flux across the membrane, and (c) dialysis at a very slow perfusion rate (57 nL/min) where the recovery is expected to be better than 90%. Using these approaches, the recovery for cocaine in the brain was found to be (8.9 +/- 0.68)%, as compared to an in vitro recovery of (5.1 +/- 0.18)% at 24 degrees C and (7.4 +/- 0.18)% at 37 degrees C, at a perfusion rate of 1.2 microL/min through a 0.3- X 2-mm microdialysis probe. The in vivo concentration of cocaine in the rat brain for an intravenous dose of 0.3 mg/kg per min was found to be 17.1 +/- 1.3 microM.

Animals↗
PubMed1992

Saliva cortisol responses to unpleasant film stimuli differ between high and low trait anxious subjects.

The present study, which aims to investigate effects of low and high trait anxiety on saliva cortisol secretion, was performed on 64 healthy male volunteers. They were assigned either to an unpleasant or a control film. Furthermore, subjects were divided within each film group by median split of their anxiety ratings into high and low trait anxious groups, resulting in four equal-sized groups. Saliva cortisol and mood ratings were the dependent variables. In contrast to the low anxious, the high anxious subjects responded with a diminished saliva cortisol response.

Adolescent↗
PubMed1992

Depot gonadotropin-releasing hormone agonist blunts the androgen-induced suppression of spermatogenesis in a clinical trial of male contraception.

Thus far, when tested as male contraceptives, GnRH agonists in combination with androgens were not very effective in producing azoospermia. Since in previous studies androgens were always given simultaneously with the GnRH agonist or later, we tested whether GnRH agonist administration after an initial androgen suppression phase might yield better results. After a control period, 3 groups of young healthy men (n = 8/group) received an initial loading dose of 400 mg 19-nortestosterone hexyloxyphenylpropionate (19NT-HPP), followed by 200 mg of the ester every 3 weeks for 24 weeks. One week after the first 19NT-HPP injection, 2 groups were given a single sc implant injection of 3.3 or 6.6 mg of the GnRH agonist buserelin, respectively, whereas a placebo implant was given to the third group. In the group receiving only 19NT-HPP, serum LH and FSH were markedly suppressed and remained low during the treatment phase. In the 16 volunteers receiving the buserelin implant LH and FSH were also suppressed on day 7, followed by a marked increase in the gonadotropins up to 2 weeks after buserelin implant injection. While LH was consistently suppressed for the remaining treatment phase, FSH returned to almost normal values in weeks 9-15. In contrast to the group treated with 19NT-HPP alone, in which sperm concentrations were reduced to oligozoospermia after only 3 weeks of treatment, the first suppressive effect in the 19NT-HPP/buserelin-treated groups was not seen before week 9. After 30 weeks, when the maximal suppression of spermatogenesis was seen, 4 of 8 volunteers in the group treated with 19NT-HPP alone were azoospermic, and the remaining 4 volunteers were oligozoospermic. In the groups treated with 19NT-HPP/buserelin, no more than 4 of 16 volunteers were azoospermic, and no more than 8 of 16 volunteers were oligozoospermic at any time point. It is concluded that GnRH agonist depot preparations have a blunting effect on the suppression of pituitary and testicular function caused by androgens in men participating in contraceptive trials.

Adult↗
PubMed1991

Autonomic, neuroendocrine, and subjective responses to emotion-inducing film stimuli.

The aim of the present study was to investigate differential emotional response patterns to film stimuli by multimodal assessment of subjective, autonomic and endocrine variables. Scenes of two films, chosen to induce different affective states, were presented to 20 male subjects. Changes in mood and perceived bodily sensations, autonomic activity (heart rate and skin conductance) and one endocrine parameter (salivary cortisol) were measured. Analyses revealed that the films elicited differential mood patterns. The cartoon film, which induced a pleasant, amused state, was accompanied by very few changes in bodily sensations, a temporary decrease in heart rate, and a rapid decrease in electrodermal activity. The suspense film, which elicited a marked reduction in joyfulness and relaxation together with an enhancement in irritation, differed from that pattern primarily by marked changes in several bodily sensations, an increase in electrodermal activity, and a temporary decrease in heart rate. There were no film-dependent cortisol changes. The results together with evidence from other emotion induction experiments support the usefulness of film segments in eliciting mild to moderate affective states and their concomitances. They particularly point to the relevance of bodily sensations as an emotional response component.

Adult↗
PubMed1991

Psychophysiological response patterns to positive and negative film stimuli.

The aim of the present study was to investigate differential emotional response patterns to film stimuli by multimodal assessment of mood ratings, perception of bodily sensations, salivary cortisol, autonomic variables and facial electromyogram (EMG). Film stimuli, chosen to elicit positive and negative emotional states, were presented to 12 subjects with general anxiety disorder and 12 controls. Changes in mood, bodily sensations, autonomic activity (heart rate, respiration rate and skin conductance), facial EMG activity (corrugator and zygomatic muscle region) and salivary cortisol were measured. Analyses revealed differential changes in all response systems except for respiration rate and salivary cortisol. Although high-anxious subjects did not differ from controls in their average responses to the negative film stimuli, there is a tendency of a greater coherence of emotional response components in generalized anxiety subjects. However, spontaneous facial expressions to positive and negative film stimuli were associated with augmented electrodermal activity within both groups. Results are interpreted in light of directional response fractionation, dimensional approaches of emotion, and the facial feedback literature.

Adult↗
PubMed1989

Stress reactions in response to the procedure of LHRH tests as measured by salivary and serum cortisol and psychological variables.

Serum and salivary cortisol release and mood ratings were measured in response to the procedure of LHRH tests in 12 medical students professionally familiarized with venipunctures (familiarized subgroup) and in 5 non-medical students (unfamiliarized subgroup). There were significant differences in cortisol and mood ratings between both groups. Only the unfamiliarized subgroup showed significant increases of cortisol and higher values for tension and anxiety. Relations between cortisol, tension and anxiety confirm an effect of experimental stress on the secretion of cortisol during the procedure of LHRH tests. Novelty and subjective mood states appear to have predictive values with regard to stress-induced endocrine reactions.

Adult↗
PubMed1985

Psychobiological profiles in infertile men.

To investigate the psychosomatic impact in male infertility, we analysed relationships among psychological (life events, personality attitudes) and biological (gonadotrophins, sex steroids, seminal parameters) variables in 101 husbands of barren couples. In patients with subnormal fertility parameters, personality attitudes were not different from those of a reference group of the questionnaire (FAPK) used in this study. However, patients with high scores on test scales such as regression, hypochondria, or emotional vacuity showed better fertility characteristics. These results agree with previously reported data, suggesting that social assertiveness and extraversion but not introversion and depression are associated with male infertility.

Adult↗
PubMed1985

Changes in saliva testosterone after psychological stimulation in men.

Saliva testosterone (ST) concentration was measured in 20 young adult and healthy men before, during and after the presentation of five different films. The films were selected to provoke erotic, sexual, aggressive, stressful and neutral stimulation, respectively. An increase in ST was found 15 min after the onset of both the erotic and the sexual stimulation, while a decline of ST levels was observed during the stressful movie showing dental surgery. No changes were found for either the neutral or the aggressive stimulant. Furthermore, no differences were found between ST levels before and after the showing of any of the films. Thus, saliva testosterone responds quickly to psychological stimulation, and may provide a practical alternative to testosterone measurements in serum under psychological test situations.

Adult↗
PubMedDate not supplied

Male infertility: relationships among gonadotropins, sex steroids, seminal parameters, and personality attitudes.

We investigated the relationship between objective psychologic test data and endocrine parameters as well as ejaculate characteristics in 117 husbands in barren couples. Data analysis suggests that test scores indicating high self-confidence, extraversion, and social assertiveness correspond negatively with male fertility parameters. Our results show that levels of gonadotropins and sex steroids, as well as accessory gland functions, are not randomly related to personality attitudes in these patients, but support the idea that psychologic factors are relevant in male infertility.

Adult↗