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Biomedical subjects

W Hussain

Publications and source records attributed to W Hussain.

18 recordsLinked to original sources
PubMed2004

Biomarker profiling of plasma from acute coronary syndrome patients. Application of ProteinChip Array analysis.

AIM: Acute coronary syndrome (ACS) is one of the leading causes of death in the world and remains a complex pathophysiologic process involving inflammatory, hemostatic and vascular processes. The purpose of this study was to identify unique proteomic biomarkers present in patients with ACS using a newly developed proteomic profiling technique, surface enhanced laser desorption/ionization (SELDI). METHODS: Citrated plasma samples obtained from clinically confirmed cases of ACS (n=100) and age matched controls (n=25) were profiled using SELDI-time of flight (TOF)-mass spectrometry (Ciphergen Biosystems, Freemont, CA, USA). A strong anion exchange (SAX) ProteinChip Array was used to profile these samples. In addition to spectra profiles, protein density plots were be obtained from the generated molecular profile. RESULTS: The SELDI profile in the molecular weight (MW) range of 0-150 kDa revealed a prominent 66.3 kDa albumin peak along with several distinct components at 28 kDa, 13.7 kDa and 6.5 kDa. Additional minor molecular components were also noted in the lower MW range (<6 kDa). There was a cluster of peaks between 10 and 12 kDa that were unique to the patients with ACS; about 1/3 of the ACS patients exhibited these peaks as evident in the ProteinChip Array spectrum. None of the age-matched controls exhibited the peaks in this MW range, nor did the normal human plasma pool that was used as an additional control. The relative intensity of these novel molecular components in the range of 10-12 kDa represent unique proteins/peptides which are generated in specific pathologic states associated with ACS. CONCLUSIONS: These observations suggest that patients with ACS have a unique cluster of molecular components that are present in their SELDI profile. It might be possible to use these patterns to identify high-risk patients who may be more susceptible to the development of unstable plaque, which may eventually lead to myocardial infarction. Identification and characterization of these molecular components will also help in the understanding of the pathogenesis of ACS. These unique peaks may represent pathologic proteins, novel inflammatory mediators or protease cleavage products. Further studies need to be done to better characterize and identify these molecular components and their pathologic role in ACS.

Acute Disease↗
PubMed1997

Management of acute respiratory infections by mothers in the community.

Home treatments are important in the management of acute respiratory infection (ARI). In order to examine how urban mothers in Lahore, managed ARI, a study was carried out within 1-km radius of a teaching hospital. A total of 50 mothers who had at least one child aged 2 months-5 years with an ARI from each of three settings-a lower class locality, a middle class locality and the outpatient department of the hospital were interviewed during the winters of 1992-93. A substantial proportion of mothers, particularly those from the lower class locality (44%), did not use home treatment for acute respiratory infection. The majority of mothers indicated that they would not use home treatment if their child had an ARI in the future. The importance of home treatment has to be introduced into the community probably by programmes conducted by health professionals.

Acute Disease↗
PubMed1995

Fulminant hepatic failure caused by tuberculosis.

A 54 year old Asian woman developed fulminant hepatic failure followed by renal failure. Because of a past history of possible tuberculosis, she was given antituberculous drugs. The chest x ray was normal. A transjugular liver biopsy showed caseating necrosis, granulomas, and acid fast bacilli indicative of miliary tuberculosis. Despite full supportive therapy, her condition deteriorated and she died. Postmortem examination showed widespread miliary tuberculosis; culture confirmed the presence of Mycobacterium tuberculosis. Tuberculosis causes fulminant hepatic failure rarely and only three cases have been described. In this, as with the other cases, hyponatraemia and hepatomegaly were features at presentation. This is the first report of treatment being given before death.

Acute Kidney Injury↗
PubMed1995

Anodic voltammetric study of ascorbic Acid at platinum and gold rotating disc electrodes.

Anodic Voltammetry of ascorbic acid at platinum and gold rotating disc electrodes has been examined in acidic and alkaline media. Ascorbic acid is oxidised by a single two electron wave at platinum and one electron two step at gold electrode. The best medium for electroanalysis is 0.1 mol 1(-1) sulphuric acid in which it shows obedience to the Levich relationship at both electrodes. The limiting current is linearly proportional to ascorbic acid concentration with Zero intercept and shows excellent rapid voltammetric determination. Electrode Kinetic parameters for mass and charge transfer have been determined. Voltammetric method has been used to determine the number of electrons involved in the reaction and the reaction mechanism has been elucidated.

Acids↗
PubMed1993

Spectrophotometric determination of natural and substituted indoles by complexing with maleic anhydr1de.

A spectrophotometric method has been developed for the quantitative determination of natural and ring-substituted indoles by complexing with maleic anhydride. The coloured complex exhibits absorbance maxima in the region 490-530 nm. The RSD of the method is 2.6%. The method is specific and simple and thus ideal for the routine analysis of indole compounds by absorbance measurements in the visible region. The analysis of each indole complex can be carried out within 15 minutes.

Journal Article↗
PubMed1993

Stabiliiy of cyanocobalamin solutions insunlight and artificial light.

A comparison of the degradation of cyanocobalamin (vitamin B(12)) solutions on exposure to sunlight and artificial light has been made using two concentrations, i.e. 950 mug/ml (corresponding to parenteral solutions) and 95 mu/ml (1:10 dilution) at pH 4.0, 5.5 and 7.0. Cyanocobalamin and the photoproduct, hydroxocobalamin (vitamin B(12b)), have been determined by a two component spectrophotometric method at 550 and 525 nm. Cyanocobalamin loss (950 mug/ml and 95 mug/ml) on exposure to sunlight for 2 hours amounts to 14.7-21.0% and 56.3-81.5% respectively indicating that light intensity is the rate determining factor in the reaction. In artificial light for 2 hours B(12) (950 mug/ml and 95 mug/ml) loses 2.7.19.4% and 1.5-27.7% respectively. Thus B(12) degradation in sunlight is greater compared to that of the artificial light, with the formation of some oxidation products, in addition to B(12b).

Journal Article↗
PubMed1993

Stability of cyanocobalamin in parenteral preparations.

The stability of seven commercial parenteral preparations of cyanocobalamin (vitamin B(12)) on storage under normal laboratory conditions for a period of twelve months has been studied using a two component spectrophotometric method for the simultaneous determination of cyanocobalamin and the degradation product, hydroxocobalamin, at 550 and 525 nm. The single ingredient vitamin B(12) preparations have been found to be stable and the potency lies within the B.P. limits. In multi-ingredient (B(1) + B(6) + B(12)) preparations cyanocobalamin is unstable and degrades from 28% to 37% with concomitant formation of hydroxocobalamin (1.7% to 25.5%) and oxidation products amounting to 56.4% +/- 9.3. Thus more than half of the vitamin content is lost during storage and these preparations are not suitable for parenteral use.

Journal Article↗
PubMed1992

Photolysis of cyanocobalamin in aqueous solution.

Cyanocobalamin is photolysed in aqueous solution to produce hydroxocobalamin. The kinetics of photolysis has been studied at pH 1-12 using a newly developed spectrophotometric method for the simultaneous determination of the two compounds at 550 and 525 nm or 361 and 351 nm. Cyanocobalamin follows zero-order kinetics at the pH values studied and the rate is catalysed by both hydrogen and hydroxyl ions. The log k-pH profile indicates that cyanocobalamin has maximum stability near pH 7. The cationic species appears to be more susceptible to photolysis than the neutral form. The rate constant for the reaction at pH 1 is 1.32 x 10(-7) mol l-1 min-1 compared with 0.050 x 10(-7) mol l-1 min-1 at pH 7.

Algorithms↗
PubMed1992

Multicomponent spectrophotometric assay of cyanocobalamin, hydroxocobalamin and riboflavin.

The photolysis of cyanacobalamin to hydroxocobalamin in aqueous solution is influenced by riboflavin. A multicomponent spcctrophotometric method has been developed for the simultaneous determination of cyanocobalamin, hydroxocobalamin and riboflavin in degraded solutions by absorbance measurements at 550, 525 and 445 nm. The relative standard deviation of the method is 2,5% and the recoveries range from 97.3-103.3%. The method has been applied to study the photolysis of cyanocobalamin in presence of riboflavin.

Journal Article↗