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Biomedical subjects

W J Clark

Publications and source records attributed to W J Clark.

17 recordsLinked to original sources

Soft tissue changes in patients with Class II Division 1 malocclusions treated using Twin Block appliances: finite-element scaling analysis.

To determine changes in soft tissue profile in patients with Class II division 1 malocclusions treated with Twin Block appliances (TBA), 99 pairs of lateral cephalographs were traced and 25 soft tissue landmarks digitized. Procrustes superimposition was used to generate average soft tissue profiles scaled to an equivalent size. Statistical differences between prepubertal, pre- and post-treatment profiles were found using ANOVA (P < 0.001). Similarly, significant differences were found between adolescent, pre- and post-treatment profiles (P < 0.001). Using a colour-coded finite element scaling analysis (FESA) programme to localize size change, male prepubertal post-treatment configurations revealed local increases in size at the labiomental groove (approximately 25%) with negative allometry (size-related form change approximately 5%) in the labial and symphyseal regions. For the female prepubertal post-treatment configuration, local increases in size were conspicuous also at the labiomental groove (approximately 6%). Male adolescent post-treatment configurations revealed increases in size in the nasal, mental, and labiomental groove regions (approximately 18%) with negative allometries associated with the upper and lower lips (approximately 20%). For the female adolescent post-treatment configuration, the labial fissure showed an increase in size (approximately 17%), whereas the upper and lower lips and symphyseal region exhibited negative allometries (approximately 5-15%). For shape change, all soft tissue post-treatment configurations were highly isotropic (uniform shape change) over the entire facial nodal mesh except in the areas of the labiomental region and the labial fissure. Thus, in children and adolescents treated for Class II malocclusions a less pronounced labiomental groove is associated with using TBAs, which may provide a more effective anterior lip seal.

Adolescent↗

Localization of mandibular changes in patients with class II division 1 malocclusions treated with twin-block appliances: finite element scaling analysis.

Thirty mandibular landmarks were digitized from cephalographs of 46 children (prepubertal, approximately 10 years old) and 53 adolescents (pubertal, approximately 13 years old) to determine mandibular morphological changes in patients with Class II Division 1 malocclusions treated with Twin-block appliances. Procrustes superimposition computed average geometries and an analysis of variance were performed on the cephalographs. Prepubertal pretreatment and approximately 13-month-posttreatment profiles and pubertal pretreatment and approximately 22-month-posttreatment profiles were statistically different (P <.002). In male prepubertal configurations, a color-coded finite element scaling analysis revealed a conspicuous area of positive allometry ( approximately 12%) in the condylar neck and negative allometry ( approximately 17%) at the apex of the coronoid process. For the female prepubertal configuration, local increases in size were discernible in the condylar neck ( approximately 3%) and in the apex of the coronoid process ( approximately 4%). Comparing male pubertal configurations, finite element scaling analysis revealed marked positive allometry ( approximately 27%) in the condylar neck and negative allometry ( approximately 16%) at the apex of the coronoid process. For the female pubertal configurations, local increases in size were noticeable at the condylar neck ( approximately 15%), with negative allometry ( approximately 9%) in the coronoid process. For shape change, all configurations were highly isotropic over the entire mandibular nodal mesh. Therefore, in growing patients treated for Class II Division 1 malocclusions with Twin-block appliances, condylar growth, coronoid process remodeling, and osteogenesis in corpus and dentoalveolar regions may reflect the correction of the underlying skeletal dysmorphology.

Adolescent↗

Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.

Poly(A)-RNA enriched for type I procollagen sequences was isolated from normal human fibroblasts and used as template to synthesize double-stranded cDNA with avian myeloblastosis virus (AMV) reverse transcriptase. After the ends had been blunted with nuclease S1 and dGMP tails had been added with terminal deoxynucleotidyltransferase, the double-stranded cDNA was annealed with pBR322 DNA that had previously been cleaved with EcoRI, blunted with AMV reverse transcriptase, and dCMP-tailed with terminal deoxynucleotidyltransferase. The chimeric molecule was used to transform Escherichia coli strain HB101. Ninety-five recombinant clones were obtained and screened by dot hybridization analysis using 32P-labeled cDNA synthesized from the original poly(A)-RNA collagen-enriched population. Three positive clones were isolated and further characterized by blot hybridization techniques and by EcoRII digestion. One clone with an insert of 2.2 kilobases was shown to contain sequences encoding for the pro-alpha 2 chain of human type I procollagen. DNA sequence analysis of a 172-nucleotide fragment demonstrated that the cloned cDNA extends from amino acid position 450 of the alpha 2 chain to the middle of the COOH-terminal propeptide.

Base Sequence↗