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W J Frajola

Publications and source records attributed to W J Frajola.

4 recordsLinked to original sources

Blood alcohol testing in the clinical laboratory: problems and suggested remedies.

Laboratory directors or others responsible for blood alcohol testing are advised to reevaluate their procedures for alcohol tests. The demand for better traffic safety has resulted in a large increase in the number of arrests for drinking and driving violations and in a concomitant increase in the litigation of alcohol-related cases. My experience in the last 20 years indicates that many difficulties in the forensic aspects of these cases and many miscarriages of justice could have been reduced or even avoided if laboratories doing alcohol analyses had followed established guidelines. The most frequently encountered problems involve the physician-patient privilege ("the test was done for medical purposes only") and the fact that alcohol results were frequently reported as blood alcohol concentrations when they were, in fact, serum alcohol concentrations. Here I discuss these problems and suggest some solutions.

Accidents, Traffic↗

A stable liquid human reference serum.

A procedure is described for the preparation of a liquid reference control suitable for use in calibration of both automated and manual chemical procedures. Pooled human serum was partially dehydrated under vacuum from the frozen state. Ethylene glycol was added to replace the water to produce a 30% solution. The stability of a liquid reference control so prepared was investigated by examining aliquots stored at room, refrigerator, and freezer temperatures for various storage times up to 568 days. Eighteen components were assayed with the Technicon Auto Analyzers SMA 6/60 and 12/60 in a tandem mode with creatinine and creatine phosphokinase channels replacing the glucose and urea nitrogen of the standard SMA 12/60. Initially specimens were examined daily, then at weekly, monthly, and longer intervals. Standard deviations and coefficients of variation were compared with those obtained on commercial lyophilized controls assayed during the same time interval. The results indicated that the ethylene glycol treatment can be used to produce a liquid reference control with stabilized enzyme activities and stable component levels.

Aspartate Aminotransferases↗