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Biomedical subjects

W Jankowski

Publications and source records attributed to W Jankowski.

At least 19 recordsLinked to original sources
PubMed1992

Uptake and subcellular distribution of intraventricularly injected [1-3H]dolichol in rat brain.

The uptake of C95[1-3H]dolichol in the form of liposomes into rat brain after the intracerebral and intraperitoneal injection was investigated. Efficient, time-dependent uptake of dolichol into the brain was observed exclusively after the intraventricular injection. Within 24 h after the injection about 10% of the applied dolichol was found in the brain and 1.5% in liver. The distribution of dolichol in various parts of rat brain decreased in the order: cerebellum greater than midbrain greater than grey matter and brain stem greater than white matter. Seven days after the injection total radioactivity in the brain decreased and concomitantly a significant increase was observed in blood circulating and liver of the rat. The highest activity was found in grey matter and it remained a few times higher in comparison with that in white matter. About 80% of the dolichol taken up by the brain membrane was recovered in the following subcellular fractions: crude nuclear fraction greater than microsomes greater than mitochondria greater than synaptosomes greater than myelin. These results demonstrate for the first time that dolichol is actively taken up by the brain membrane exclusively after intraventricular injection of dolichol-phosphatidylcholine in the form of liposomes; this method may be useful in studies on the role of dolichol in brain function.

Animals↗
PubMed1989

Subcellular compartmentation of dolichol taken up by mouse leukemia cells.

[1-3H]Dolichol was intraperitoneally incubated with mouse leukemia Lcb 35 cells. The radioactive lipid taken up by the cells was subsequently recovered in two fractions separated by sucrose density gradient centrifugation: a particular, most probably, lysosomal fraction and a cytoplasmic one. Thin-layer chromatography of radioactive lipids present in both fractions revealed that majority of dolichol was unchanged and only a small part esterified with fatty acids. Distribution of dolichol in various mouse organs was examined. Of all the organs examined, spleen accumulated the highest amount of radioactivity.

Animals↗
PubMed1981

Uptake and metabolism of polyprenols by animal cells cultured in vitro.

Several mammalian, chicken, and mosquito cells grown in vitro take up tritiated dolichol supplied to the incubation medium. The extent of labelling varied markedly between different cell cultures. After 20 h incubation most of the dolichol taken up was unchanged and the major product of metabolism of dolichol was identified as its fatty acid esters. Green-monkey kidney cells were tested with 8 fully unsaturated and 6 alpha-saturated polyprenols ranging from C35 to C105. In general the uptake of alpha-saturated polyprenols (dolichol type, was higher. Considerable differences were found between the uptake of polyprenols of differing chain lengths. Less than 1% of the polyprenols taken up was converted into more polar product, mainly polyprenyl phosphates and polyprenyl phosphate sugars. The short-chain polyprenols, from C35 to C65, were metabolized more rapidly than the long-chain polyprenols, as judged from the amount of polar products and fatty acid esters of polyprenols.

Animals↗
PubMed1976

C55-Dolichol: occurrence in pig liver and preparation by hydrogenation of plant undecaprenol.

Apart from the long-chain dolichols (C80-C110), pig liver contains also a family of much shorter polyprenols with dominating C55-polyprenol. This compound was identified as cis/trans-dihydroundecaprenol in which the OH-terminal isoprene residue was saturated. The number of internal trans isoprene residues in this compound was three in comparison with two such residues in long-chain C95-dolichol. Chemical preparation of dihydroundecaprenol with a selectively saturated OH-terminal isoprene residue from fully unsaturated plant undecaprenol is presented.

Animals↗
PubMed1976

Phage-dependent changes in Shigella flexneri type antigen synthesis.

Lysogenic conversion of Shigella flexneri type antigens was studied with the aid of wild-type and thermosensitive mutant phages. With all wild-type phages, the appearance of glycosylated antigen was accompanied by the appearance of polyprenyl phosphate glucose synthetase activity. With some of the mutant phages, the appearance of glycosylated antigen was not followed by the formation of lipid-linked glucose in the enzyme assay. The reverse has also been observed, i.e., the high rate of formation of lipid-linked glucose and the lack of V-type antigen.

Antigens, Bacterial↗