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Biomedical subjects

W Jensen

Publications and source records attributed to W Jensen.

At least 19 recordsLinked to original sources

Acute peripheral nerve recording characteristics of polymer-based longitudinal intrafascicular electrodes.

We examined the recording characteristics of two different types of polymer-based longitudinal intrafascicular electrodes (LIFEs) in peripheral nerve: single-stranded (s-polyLIFEs) and multistranded (m-polyLIFEs). Recordings were also made from Pt-Ir wire-based electrodes (PtIrLIFEs) as a control. The electrodes were implanted in either tibial or medial gastrocnemius branches of the rabbit sciatic nerve, and in the sciatic nerve of rats. Recorded neural activity induced by manually elicited afferent neural activity showed that both polyLIFE versions performed comparably to PtIrLIFEs.

Action Potentials↗

Neuro-fuzzy extraction of angular information from muscle afferents for ankle control during standing in paraplegic subjects: an animal model.

This paper is part of a project whose aim is the implementation of closed-loop control of ankle angular position during functional electrical stimulation (FES) assisted standing in paraplegic subjects using natural sensory information. In this paper, a neural fuzzy (NF) model is implemented to extract angular position information from the electroneurographic signals recorded from muscle afferents using cuff electrodes in an animal model. The NF model, named dynamic nonsingleton fuzzy logic system is a Mamdani-like fuzzy system, implemented in the framework of recurrent neural networks. The fuzzification procedure implemented was the nonsingleton technique which has been shown in previous works to be able to take into account the uncertainty in the data. The proposed algorithm was tested in different situations and was able to predict reasonably well the ankle angular trajectories especially for small excursions (as during standing) and when the stimulation sites are far from the registration sites. This suggests it may be possible to use activity from muscle afferents recorded with cuff electrodes for FES closed-loop control of ankle position during quite standing.

Algorithms↗

Effect of initial joint position on nerve-cuff recordings of muscle afferents in rabbits.

The objective was to characterize nerve-cuff recordings of muscle afferents to joint rotation over a large part of the physiological joint range. This information is needed to develop control strategies for functional electrical stimulation (FES) systems using muscle afferent signals for sensory feedback. Five acute rabbit experiments were performed. Tripolar cuff electrodes were implanted around the tibial and peroneal divisions of the sciatic nerve in the rabbit's left leg. The electroneurograms (ENG) were recorded during passive ankle rotation, using a ramp-and-hold profile starting at seven different joint positions (excursion = 5 degrees, velocity = 10 degrees/s, initial positions 60 degrees, 70 degrees, 80 degrees, 90 degrees, 100%, 110 , and 120 ). The amplitude of the afferent activity was dependent on the initial joint position. The steady-state sensitivity of both nerve responses increased with increasing joint flexion, whereas the dynamic sensitivity increased initially but then decreased. The results indicate that recordings of the muscle afferents may provide reliable information over only a part of the physiological joint range. Despite this limitation, muscle afferent activity may be useful for motion feedback if the movement to be controlled is within a narrow joint range such as postural sway.

Afferent Pathways↗

Nerve cuff recordings of muscle afferent activity from tibial and peroneal nerves in rabbit during passive ankle motion.

Activity from muscle afferents regarding ankle kinesthesia was recorded using cuff electrodes in a rabbit preparation in which tactile input from the foot was eliminated. The purpose was to determine if such activity can provide information useful in controlling functional electrical stimulation (FES) systems that restore mobility in spinal injured man. The rabbit's ankle was passively flexed and extended while the activity of the tibial and peroneal nerves was recorded. Responses to trapezoidal stimulus profiles were investigated for excursions from 10 degrees to 60 degrees using velocities from 5 degrees/s to 30 degrees/s and different initial ankle positions. The recorded signals mainly reflect activity from primary and secondary muscle afferents. Dorsiflexion stretched the ankle extensors and produced velocity dependent activity in the tibial nerve, and this diminished to a tonic level during the stimulus plateau. The peroneal nerve was silent during dorsiflexion, but was activated by stretch of the peroneal muscles during ankle extension. The responses of the two nerves behaved in a reciprocal manner, but exhibited considerable hysteresis, since motion that relaxed the stretch to the driving muscle produced an immediate cessation of the prior stretch induced activity. A noted difference between the tibial and peroneal nerve responses is that the range of joint position change that activated the flexor afferents was greater then for the extensor afferents. Ankle rotation at higher velocities increased the dynamic stretch evoked responses during the stimulus ramp but showed no effect on the tonic activity during the stimulus plateau. Prestretching the muscles by altering the initial position increased the response to the ramp movement, however, for the peroneal nerve, when the prestretch brought the flexor muscles near to their maximal lengths, the response to additional stretch provided by the ramp movement was diminished. The results indicate that the whole nerve recorded muscle afferent activity may be useful for control of FES assisted standing, because it can indicate the direction of rotation of the passively moved ankle joint, along with coarse information regarding the rate of movement and static joint position.

Afferent Pathways↗

The effect of moderate levels of simulated altitude on sustained cognitive performance.

Previous work has documented cognitive deficits at high altitudes (15,000-25,000 ft), but there is controversy for lower altitudes. This study looked at the effects of moderate altitudes--12,500 ft and 15,000 ft--on short-term memory in comparison to 2,000 ft. Seventy-two student pilots and instructors were first administered the Vocabulary, Digit Span, and Digit Symbol subtests from the Wechsler Adult Intelligence Scale-Revised, the Vandenberg Mental Rotation Test, and the near-contrast sensitivity portion of the Vistech VCTS 6000 chart. Participants then spent 1 1/2 hr at their designated altitude for cognitive testing. Participants performed a 30 min vigilance task while listening to an audiotape with instructions to recall radio calls prefaced by their assigned call sign. Half of the radio calls were high memory loads (at least 4 pieces of information), and half were low memory loads (no more than 2 pieces of information). No effects of altitude were found in performance on the Vigilance task. However, for readbacks of high memory load, significant deficits in recall were observed at 12,500 ft and 15,000 ft, whereas no effect of altitude was observed on recall of readbacks with low memory loads. These results indicate that, at altitude, short-term memory was exceeded for the readbacks requiring a larger amount of information to be recalled, and that cognitive deficits are found at lower altitudes than previously observed.

Adult↗

Causal probabilistic network and power spectral estimation used in sleep stage classification.

A new method for sleep-stage classification using a causal probabilistic network as automatic classifier has been implemented and validated. The system uses features from the primary sleep signals from the brain (EEG) and the eyes (AOG) as input. From the EEG, features are derived containing spectral information which is used to classify power in the classical spectral bands, sleep spindles and K-complexes. From AOG, information on rapid eye movements is derived. Features are extracted every 2 seconds. The CPN-based sleep classifier was implemented using the HUGIN system, an application tool to handle causal probabilistic networks. The results obtained using different training approaches show agreements ranging from 68.7 to 70.7% between the system and the two experts when a pooled agreement is computed over the six subjects. As a comparison, the interrater agreement between the two experts was found to be 71.4%, measured also over the six subjects.

Adult↗

Expression of CD43 epitopes on NK and T cells.

CD43 epitope expression was studied with a panel of monoclonal antibodies (MoAbs) by immunohistochemistry on freeze sections of lymphoid tissues. The MoAb WEN3 stained most cells weakly in the T areas and scattered splenic red pulp cells strongly, whereas the other MoAbs strongly stained the majority of the cells in the T areas but gave variable staining patterns of cells in the non-T areas. Flow cytometry on CD4+ and CD8+ T cells (T4 and T8 cells), freshly isolated NK cells and LAK cells showed distinct staining profiles for each cell type, with epitope expression patterns of T8 cells lying between those of T4 cells and NK/LAK cells. T8 cells were split by one of the MoAbs, the NK cells, but not LAK cells, were split by two other MoAbs.

Animals↗

Perforation of the terminal ileum with cytomegalovirus vasculitis and Kaposi's sarcoma in a patient with acquired immunodeficiency syndrome.

This case report documents a perforation of the terminal ileum in a 40-year-old white male homosexual with the acquired immunodeficiency syndrome. The perforation occurred at a site that had severe cytomegalovirus infection and was in close proximity to multiple nodules of Kaposi's sarcoma. The ileum showed multiple deep ulcers with large numbers of cytomegalovirus inclusions and vasculitis with infected endothelial cells, small-vessel thrombosis, focal disruption, and hemorrhage. We review the evidence that cytomegalovirus infection--and not Kaposi's sarcoma--was responsible for this perforation and, in light of the new medical therapy for such infections, should be regarded as an important cause of gastrointestinal perforation in patients with the acquired immunodeficiency syndrome.

Acquired Immunodeficiency Syndrome↗

Drug and alcohol use by disabled and nondisabled persons: a comparative study.

The study is an initial attempt to provide comparative alcohol and drug use data on disabled and nondisabled persons. The lack of useful information on disabled persons is described in the literature review. Sixty-six disabled students and 115 nondisabled students returned questionnaires which examined their alcohol and drug use. The two groups were compared using tests of significance. The data provided in this report tend to confirm the notion that the disabled and nondisabled students use alcohol and drugs in similar ways. Caution is urged against generalization, and the need for additional studies is emphasized.

Adolescent↗

Hemodynamic measurements in a sheep model with a hollow fiber artificial kidney containing modified cellulose.

HFAK-RC caused pronounced leukopenia, increase in TXB2 levels in plasma and hemodynamic pressure changes as a reflection of complement activation during EC in sheep. In contrast no increase in TXB2 levels and no changes in hemodynamics are observed with HFAK-MC. The leukopenia and granulocytopenia in the latter is much less pronounced and probably reflects the phenomenon "frustrated phagocytosis".

Animals↗

Response of protein C and protein C inhibitor to warfarin therapy in patient with combined deficiency of Factors V and VIII.

The role of Protein C in combined factor V/VIII deficiency was examined by reducing the Protein C concentration using warfarin therapy in a patient with the combined deficiency. The factor VIII deficiency was like Hemophilia-A, with deficiency of VIII:C and VIII:C(Ag), but normal VIIIR:Ag and VIIIR:cof. The factor V deficiency was due to loss of the V antigen. During warfarin therapy the Protein C level was reduced, but concentrations of factors V and VIII did not change. Protein C Inhibitor was normal throughout. Thus combined factor V/VIII deficiency is not related to Protein C levels.

Adult↗