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Biomedical subjects

W Jost

Publications and source records attributed to W Jost.

34 records · Page 2Linked to original sources
PubMed1991

[Polyvalent immunoglobulins in prevention of viral infections in children with neoplastic diseases treated with immunosuppressive therapy].

31 children with leukemias, lymphomas and solid tumors treated by polychemotherapy received prophylactic i.v. polyvalent immunoglobulins during 48 hours after exposion with varicella-zoster-virus, rubella-virus or mumps-virus. None of the 23 children with immunity against the exposed virus developed the infection. 8 children had no antibodies against the exposed virus. The prophylaxis with the polyvalent immunoglobulin protected them against chickenpox (6 children) and rubella (2 children). All children showed an elevation of the IgG level in plasma and the antibody titers against measles-, mumps-, rubella- and varicella-zoster-virus after application of the immunoglobulin. A study with a larger number of patients is necessary to confirm our results, that polyvalent immunoglobulins with high titers against exposed viruses are effective in prevention of this diseases in immunocompromised children.

Adolescent↗
PubMed1991

[Subjectively experienced anxiety as an aspect of coping with illness in children with malignant diseases].

The present paper focuses the perceived anxiety of pediatric oncologic patients (N = 30) at the time of medical treatment. Comparison with a control group of not chronically ill patients (N = 20) results: Children and adolescents with oncologic diseases are reporting significant less anxiety; this is relatively independent of the asked objects and situations. The invasiveness of treatment (applied chemotherapy or not) does not show any influence on the amount of perceived anxiety of oncological patients. Based on current empirical and conceptual findings of coping with serious illnesses the self reported diminished anxiety of pediatric oncologic patients is termed intraphysical "denial" coping mode. The issue of adaptivity is discussed. Finally, initial consequences for psychosocial care of pediatric oncologic patients are described.

Adaptation, Psychological↗
PubMed1990

Induction of an Extracellular Ribonuclease in Cultured Tomato Cells upon Phosphate Starvation.

Suspension-cultured cells of tomato (Lycopersicon esculentum) start to secrete an RNA-degrading enzyme activity during transition from logarithmic to stationary growth phase. Using affinity chromatography on agarose-5-(4-aminophenyl-phosphoryl) uridine 3'(2') monophosphate as a powerful and final enrichment step, the enzyme was purified to homogeneity and characterized as ribonuclease I (RNase I) according to the following data: (a) it has an M(r) of 22,000 (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), a pH-optimum of pH 5.5, a pl of 3.9, and its activity was found to be insensitive to EDTA; (b) the enzyme splits single-stranded RNA endonucleolytically by a phosphotransferase reaction yielding 2',3'-cNMPs as primary monomeric products; (c) as studied with diribonucleoside monophosphates as substrates, the enzyme exhibits a pronounced preference for 5' purine residues adjacent to the cleavage site. Most interestingly, in vivo synthesis and secretion was found to be induced when tomato cells were specifically starved for phosphate as mineral nutrient. (a) Extracellular enzyme activity increased about tenfold after transfer of phosphate-grown cells into medium lacking only phosphate. Accordingly, this increase in activity was not detectable when cells were constantly supplied with phosphate. (b) Biosynthetically labeling of the extracellular protein with radioactive amino acids was detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis/fluorography directly within the bulk of extracellular proteins. Therefore, we propose that the secreted tomato RNase I synthesized upon phosphate starvation is a component of a higher plant inducible rescue system for scavenging exogenous phosphate.

Journal Article↗
PubMed1990

[Intraspinal, extradural hemorrhage in a 7-year-old boy with hemophilia B].

A 7-year-old boy with severe hemophilia B suffered an intraspinal extradural hemorrhage without preceding trauma. Since the age of 3 years the patient is HIV-1 positive. The actual hemorrhage caused a strong pain in the thoracolumbar area without signs of spinal cord compression. It was diagnosed by CT-scan. By early high dose factor-IX substitution the hemorrhage was stopped and no neurological complications occurred. The further substitution regime is described.

Child↗
PubMed1990

[Status of plasmapheresis and cyclosporin A in the treatment of systemic lupus erythematosus].

We report the history of 2 teenagers suffering from systemic lupus erythematosus for more than 5 years. Both of them were treated with total plasma exchange (TPE) and Ciclosporin A. They responded well to this therapy and achieved remissions. Especially the 2nd patient showed a dramatic clinical improvement after a relapse caused by sun exposure. Both treatment modalities are discussed and the following conclusions are drawn: TPE is an important therapeutic element in treating patients with SLE, whereas Ciclosporin A is not recommended for therapy of first choice. Further prospective and controlled studies have to show, if there is a benefit of this therapy, especially in childhood.

Adolescent↗
PubMed1983

A modified high-performance thin-layer plate for the separation of purines and pyrimidines.

Several ways of using the recently developed high-performance thin-layer chromatography (HPTLC) precoated plate NH2 F 254s to separate purines and pyrimidines are described. This precoated plate is coated with silica gel 60 which has been chemically modified with alkylamino groups. In view of the chemical properties of the functional groups bonded to the silica gel matrix, the HPTLC precoated plate NH2 F 254s can be considered to be a weak basic ion-exchange plate. In aqueous eluants the substances are separated principally according to charge differences. The HPTLC precoated plate NH2 F 254s can, however, also be used to separate uncharged, polar compounds with organic solvents. Examples of separations and chromatograms for its use in both aqueous and organic eluants are given.

Chromatography, Thin Layer↗
PubMedDate not supplied

[Myositis caused by a mycoplasma infection].

UNLABELLED: Mycoplasma pneumonia infection can be associated with neurological manifestations such as meningoencephalitis, cerebellitis, aseptic meningoitis, polyradiculopathy, transverse myelitis, cranial nerve palsies and myositis [4, 5]. We report a case of a white female 11 years, 2 months old child, who presented with a 3 day history of pain in the left leg. The electromyograpy showed pathological signs. We found a serological titer of IgM antibodies for Mycoplasma pneumoniae. By treatment with erythromycin the complaints improved quickly. CONCLUSION: A myositis can be caused with an infection with Mycoplasma pneumoniae. The differential diagnosis is essential.

Antibodies, Bacterial↗
PubMedDate not supplied

[The effect of methotrexate pharmacokinetics and of leucovorin rescue on the prognosis of osteosarcoma].

A total of 129 high-dosage methotrexate therapies performed in 19 patients with osteosarcoma were retrospectively analyzed. Serum methotrexate peak concentrations were found to vary widely, both inter-individually as well as in the same patient. The measured MTX peak concentrations correlated closely with pharmacokinetic data such as area under the curve and total body clearance. No correlations were found between the serum MTX correlations and different times after methotrexate administration. Increase in leucovorin rescue or low MTX peak concentrations were associated with poor prognosis. High-dosage methotrexate therapies with leucovorin rescue need to be further optimized in accordance with biochemical knowledge of the mode of action and the individual pharmacokinetic data of methotrexate. Such optimization may be expected to improve the prognosis for osteosarcoma. Serum methotrexate concentrations should be determined not only 24, 48, and 72 hours after methotrexate administration, in order to avoid elevated toxicity of the therapy, but also at the start of methotrexate infusion, in order to influence MTX peak concentrations at an early stage if necessary. Measurement of L-leucovorin in serum will be necessary, to enable a restrictive leucovorin rescue to be performed safely.

Adolescent↗