PubMed Health⌕ Search

Biomedical subjects

W K Latshaw

Publications and source records attributed to W K Latshaw.

6 recordsLinked to original sources
PubMed1993

Morphological evidence of cyclic changes observed in Leydig cells of a chimeric mouse.

Light and electron microscopy were employed in studying morphologic heterogeneity in Leydig cells (LC) in situ, as manifested in a chimeric mouse. This mouse was chosen to take advantage of increased LC concentration in territories of agametic tubules (Hrudka et al., 1989). Large number of LC's, side by side, gave better opportunity to compare their size, shape, organelle representation, surface specialization and mutual interaction. Because cell organelles and inclusions were distinctive, we were able to monitor their dynamics and changes in cell shape and size, as well as phenomena in the intercellular space. In spite of an apparent continuum, a series of definite forms or stages were identified, in the course of the study. After arranging these reproducible and predictable forms in a logical sequence, the following, tentative cycle of secretory activity of LC is proposed: 1) stage of transient or 'lean' cell, 2) stage of SER proliferation and cell hypertrophy, 3) stage of lipid deposition or 'fat' cell and 4) stage of regression.

Animals↗
PubMed1989

Co-existence of gametic and agametic seminiferous tubules in a chimeric mouse. A light- and electron-microscopic study.

Six chimeras, including 4 phenotypic males and 2 females, were produced by aggregation of F1 (C57BL x BALB/c) and Swiss white embryos. All were fertile, except 1 male, whose deviation in testicular structure prompted this light- and electron-microscopic study. This chimera had a well-developed sperm-conducting system, sperm in the epididymis and active accessory sex glands. The testes displayed typical parenchymal and stromal components with the important exception of co-existence of gametic and agametic seminiferous tubules. These tubules were organized in territories of quasi-lobular configurations which appeared to open separately into rete testis. The former corresponded to normally developed and active seminiferous tubules, while the latter were solid testicular cords devoid of any germ cells and embedded in solid masses of interstitial (Leydig) cells. Special mitochondrial transformations were identified in sustentacular (Sertoli) cells of both types of tubules, in maturing spermatids and sperm. These and other submicroscopic sperm defects might be the cause of infertility.

Animals↗
PubMed1973

The vascular architecture of the porcine small intestine.

The vascular anatomy of the porcine small intestine was studied by injection of intestinal vessels with India Ink. Examination of transverse and longitudinal serial sections of the injected intestine facilitated a three-dimensional interpretation of the vascular pattern. An artery from the mesentery penetrated the tunica muscularis, supplied muscular branches and passed on to the submucosa where it formed an arterial rete. From the submucosal arteries, arterioles arose and followed a direct axial course to the tips of villi where they ramified into a subepithelial capillary plexus. Some of the capillaries, at the midpoint of the villus, fused into paraxial venules, which emptied into a "transverse venule" at the base of the villus. Other villus capillaries were continuous with those of the crypts. The pericryptal capillary plexus received a few arterial branches from the submucosal arteries. The transverse venule and the pericryptal capillary plexus emptied into large, segmentally dilated veins in the submucosa. The submucosal veins formed an extensive anastomosing network drained by large venous trunks which passed through the muscle layers to the mesentery. The observations suggest possible relationships between the vascular pattern and intestinal fluid movement.

Animals↗