Proceedings: Auto-instruction in histology and cell biology as a substitute for traditional laboratory activities.
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Biomedical subjects
Publications and source records attributed to W K Metcalf.
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1. A systematized computer technique of centroid analysis has been developed to evaluate the precise geometric relationship between a muscle and a bone and it has been applied to the abductor mechanism of the human hip joint. 2. The validity of the traditional use of straight lines for displaying the line of action of a muscle is open to serious question, although in the case of the gluteus medius the straight line simplification is not unmeasurable. 3. The determination of the precise interactions of muscles and bones is of major importance in the consideration of various orthopaedic procedures.
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The reduced PHA responsiveness of human lymphocytes obtained from heparinized as compared to defibrinated blood has been shown to be due to platelet contamination in the former. Inhibition of blastoid transformation and lymphocyte death is directly related to the number of platelets added to a culture. Divalent ions partially reduce this platelet inhibitor phenomenon but do not block if completely. The "toxic" platelet components appear to be localized in the membranes and particulate matter after homogenization and hard centrifugation. Comparative studies of PHA transformation must control platelet contamination of the cultures in order to avoid severe difficulties of interpretation.
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