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W Köhler

Publications and source records attributed to W Köhler.

At least 181 records · Page 10Linked to original sources
PubMed1980

[T-proteins of Streptococcus pyogenes. I. Communication: Preparation of a serological type specific T1-antigen by ion exchange chromatography and its characterization (author's transl)].

The T-protein of Streptococcus pyogenes type 1 was trypsin-extracted and subsequently purified by ion exchange chromatography on CM and DEAE cellulose. Fractionation on CM cellulose by stepwise increase of the pH did not result in separation of type specific material from cross reacting components. The bulk of serologically active material was eluted at pH 5.6. On DEAE cellulose a type specific fraction was eluted with 0.05 m phosphate buffer, pH 8.2. A second fraction eluted with 0.25 m NaCl in the same buffer contained type specific as well as cross reacting material. Molecular weight distributions of type-specific T-protein were studied by gel chromatography on Ultrogel ACA44 and Biogel A 1.5 m. A multiple size subunit structure of T-protein was found and supported by SDS electrophoresis. Molecular weights of fragments serologically active in double diffusion were detected in a range of 37 000 to more than 200 000 Dalton. The isoelectric point was determined as to be pH 4.5. The purified T-protein was found to be free of cystein and of the amino sugars N-acetyl-glucosamine and N-acetyl-muramic acid.

Amino Acids↗
PubMed1980

Purification and characterization of erythrogenic toxins. II. Communication: in vivo biological activities of erythrogenic toxin produced by streptococcus pyogenes strain ny-5.

Some in vivo biological activities of a purified erythrogenic toxin preparation from strain NY-5 of group A streptococci (ET NY-5) were studied. After intracutaneous administration in a dose of minimally 5 X 10(-8) mg, the toxin provoked a skin reaction in old guinea pigs. No skin reaction could be elicited in young adult rabbits. ET NY-5 was pyrogenic for rabbits; the MPD-4 amounted to 0.017 microgram/kg intravenously. The pyretic response had a delayed onset and then rose gradually up to hour 5 postinjection. Intravenous administration led to changes in circulating leukocyte counts, viz. brisk acute granulocytopenia and protracted lymphopenia. The method of pyrogenic cross tolerance revealed that ET NY-5 contains two types of pyrogenic exotoxin, A and C.

Animals↗
PubMed1979

[Importance of skin contamination in blood culture readings].

The importance of the skin contamination for the results of blood cultures was emphasized by model examinations. In the method of blood taking without previous desinfection of the skin the quota of positive blood cultures increased by the twofold to threefold per culture and test person (5.7 to 18.8% and 11.3 to 26.3%, respectively). In large-volume blood takings the contamination rate becomes smaller with increasing blood volume. The rejecting of a first blood sample is to be recommended, when the possibility is given. With an increased quantity o blood per taking by blood bactericidia a decreased contamination rate is to be expected. By the results of the examinations the necessity of a consequent desinfection of the skin is to be emphasized, also when closed systems of blood cultures are used.

Bacteria↗
PubMed1979

[Bacteriaemia during parturition (author's transl)].

Bacteriological tests were made of 606 blood cultures which had been obtained from 64 women in labour. Positive findings were recorded from 8.1 per cent, whereas another positive percentage established before delivery amounted to 4.7 only. The percentage of bacteriaemia findings was not substantively increased following electronic monitoring of childbirth. Beta-haemolysing streptococci were found to grow in 7.8 per cent of vaginal smear cultures taken from women in labour and in six per cent of cultures taken from newborns. Amniotic fluid was also bacteriologically tested during labour.

Amniotic Fluid↗
PubMed1979

[The relationship between haptoglobin type and serum agglutination titers against group G streptococci with the T4-antigen in pregnant women and newborn infants].

The relationship between haptoglobin type and titer of agglutination against streptococci carrying the T4-antigen was examined in 100 pairs of sera (mother/child). In healthy pregnant women high titers were predominant in individuals of haptoglobin type 2--2. Lower titers were found in sera of women with diseases during pregnancy. In 4% of the newborns only the haptoglobin type could be recognized. In comparison of maternal and neonate titers of agglutination pointed out the antibody-like activity of haptoglobins.

Agglutination Tests↗
PubMed1979

[Further investigations concerning the reaction between haptoglobin and T4-antigen-carrying streptocci (author's transl)].

The median level of haptoglobin types 2-2 and 2-1 was found to be proportional to the agglutination titer of T4 antigen-carrying streptococci (Fig. 1). This relationship need not exist in individual sera since, as seen from Table 1, high agglutination titers may be caused by sera with low levels of haptoglobin. Thus the agglutination reaction might depend not only on the quantity of haptoglobin but also on other factor(s) (at least in individual serum samples). - On the other hand, different agglutination titers did not correlate with the quantity of T4 antigen either. A strain of Strep. pyogenes, type 60, was agglutinated at high titers by sera with a high level of haptoglobin in spite of its low ability to absorb haptoglobin. This was in contrast to a strain of group G (20488) which had a high capacity both to become agglutinated and to absorb haptoglobin (Table 2). Absorption of haptoglobin by affinity chromatography decreased the agglutination titer for T4 streptococci. - The reaction between haptoglobin and T4-streptocci did not fix complement. No differences were found between sera of haptoglobin types 1-1, 2-1, and 2-2 with respect to the amount of C, C3, C4, and C3A, respectively (Tables 3-5).

Agglutination Tests↗
PubMed1979

Haptoglobins act similar to antibodies. Steps of discovery. Analogous conclusions from pure preparations, umbilical cord sera and animal sera.

The haptoglobins in man and in mammals are agglutinin-like-substances for streptococci having the T4 antigen. The kind of the agglutinin-like behaviour depends on the genetic type of the haptoglobins. Haptoglobins of the types Hp 2-2, Hp 2-2 like and Hp 2-1 are hightitred complete agglutinins (titre in the range between 1:200 up to 1:3000 and higher) whereas haptoglobins of the type Hp 1-1 are "blocking antibodies". - The steps of the discovery and evidence are presented in detail. Different questions dealing with the clinical significance are discussed.

Agglutination Tests↗
PubMed1979

[The heterogeneity of streptokinases].

Purified streptokinases of some strains of different types of Strep. pyrogenes (group A) as well as streptokinases of a strain of group C or group G streptococci, resp., were studied in regard of their heterogeneity. Differences of the streptokinases were found in their amino acid compositions and in isoelectric points. Serological differences were detected by a quantitative precipitation reaction with fluorescein-coupled antistreptokinase antisera from the goat as well as by neutralization reactions of the activator activities of the streptokinases by antistreptokinase antisera obtained from guinea pigs.

Amino Acids↗
PubMed1978

[Lymphocyte transformation test with streptococcal antigens in newborn infants and their mothers].

Following streptococcal mitogens and antigens were tested in the lymphocyte transformation test with human cord blood and maternal blood: streptococcal M1- and M19-proteins, mucopeptide, erythrogenic toxins and additional phytohemagglutinin. There are no significant differences in the blastogenic response with the different mitogens and antigens in maternal and cord blood. The cord blood lymphocytes show the same degree of blastogenic reaction to the mitogens and antigens as the maternal lymphocytes, that may reflect the state of readiness for cellular immunological defence. The spontaneous incorporation of 3H-labelled thymidine into cord blood lymphoblasts is significant higher than in lymphoblasts of maternal blood.--The results of specific and unspecific stimulation by means of steptococcal antigens are discussed.

Adult↗
PubMed1978

[B-streptococci infection in the newborn (author's transl)].

The authors describe their experiences with the treatment of 3 newborns with meningitis due to B-streptococci,, and give a review of the problems of etiology, clinical symptoms, diagnosis and therapy of infections with B-streptococci in newborns. Furthermore, the article deals withthe frequency of the ocurrence of these bacteria in mothers, children and in the medical personal staff as well as with the possible ways of transmission. The relations between infections with B-streptococci and respiratory distress syndrome in newborns are discussed with the problems of differential diagnosis and therapy.

Cross Infection↗