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W Köhler

Publications and source records attributed to W Köhler.

At least 91 records · Page 5Linked to original sources
PubMed1989

[Streptococcus-induced syndrome of toxic shock (streptococcal toxic shock syndrome)].

Report on the first case of streptococcal toxic shock syndrome in the GDR. The patient was a 54-year-old female. One week before admission to the hospital she cut her finger. The day before admission to the hospital she presented with a painful left shoulder. Demarcation followed, and Streptococcus pyogenes (group A streptococci) was isolated from this area. The temperature rose to more than 40 degrees C and she became confused, hypotensive and anuric. There was evidence for disseminated intravascular coagulation. She died 23 h after admission. Clinical course and laboratory parameters resembles staphylococcal toxic shock syndrome, except a diarrhoea. The streptococcal strain produced a large amount of erythrogenic toxin type B (more than 20 ng/ml), but not erythrogenic toxins A or C. Erythrogenic toxins of Streptococcus pyogenes seem to play the same role in the development of streptococcal toxic shock syndrome as the toxic shock syndrome 1 (TSST-1) in staphylococcal toxic shock syndrome.

Animals↗
PubMed1989

[Type 2 Streptococcus suis (R-Streptococci) as pathogens of occupational diseases. Report of a case and a review of the literature].

Report on the second human case of a Streptococcus suis type 2 (group R streptococci) infection in the GDR. The patient, a 33-year-old butcher, fell ill with an acute meningitis and an initial myocarditis. Group R streptococci were isolated from cerebrospinal fluid and blood. The patient was treated with penicillin G, ampicillin and gentamicin, followed by trimethoprim-sulfamerazine. Both, meningitis and myocarditis disappeared and the patient recovered completely. The disease was recognized as an occupational disease. Review of the literature. Since 1968 108 cases of human S.suis type 2 infections were described, mostly as meningitis. In most of the cases a close contact to pigs could be confirmed.

Adult↗
PubMed1989

Dose-response function for Painter's SCE-model.

The replication model for sister chromatid exchange (SCE), when introduced in 1980 by Painter, was claimed to be consistent with the one hit property of SCE. However, the argument offered in favour of the one hit property was based on a defective dose-response function, as shown in this paper, since dose as the independent parameter of any dose-response function was not included in the considerations. This missing part of the model's dose-response function is added and, by using Bessel functions, a formula for the complete dose-response function is presented. A re-examination of the newly derived function shows that, in the model, linearity holds only under certain restricted circumstances.

Humans↗
PubMed1989

Reactions of the lectin anti-AHP from the edible snail Helix pomatia with N-acetyl-D-galactosamine of streptococci.

The lectin anti-AHP obtained from the albumen gland of the edible snail, Helix pomatia, was used for the routine identification of group C streptococci. Without any exception all 1,045 strains of group C streptococci (S.equisimilis, and S.zooepidemicus, and S.dysgalactiae) were agglutinated by the Helix lectin, but none of the 12,264 strains of group A, 1,346 strains of group G, 330 strains of group B as well as that of other streptococcal groups. The presence of type-specific T antigens of group A streptococci in group C streptococci does not influence the agglutinability of S.equisimilis (group C). A few but not all strains of S.milleri either carrying or not group G specific carbohydrate and S.sanguis with group H antigen were agglutinated by the Helix lectin Anti-AHP. This suggests that terminal N-acetyl-D-galactosamine of these bacteria reacted with the Helix lectin (Anti-AHP). Therefore the Helix lectin is very useful tool for identification of group C streptococcus as well as for immunological studies.

Acetylgalactosamine↗
PubMed1988

Investigations on the binding of erythrogenic toxin A of Streptococcus pyogenes on human peripheral blood lymphocytes. II. Identification of toxin-binding lymphocytes and characterization of the receptor.

Gold complexes of erythrogenic toxin A (ETA) of Streptococcus pyogenes (strain NY-5) were bound to human lymphocytes that could be characterized as T cells by rosetting or labelling with OKT 3 antibody and anti-mouse-IgG-gold. Using OKT 4 and OKT 8 antibodies, respectively, it was demonstrated that binding of ETA was confined to the T helper/inducer cell subpopulation. Treatment of lymphocytes with neuraminidase enhanced the binding of ETA-gold, whereas trypsin treatment resulted in its reduction. Gangliosides of type II or type III and D-galactose only slightly decreased the ETA-gold binding. Preincubation of living lymphocytes with the mitogens concanavalin A or the Lens culinaris-lectin enhanced the binding of ETA-gold.

Bacterial Proteins↗
PubMed1988

Biological and biochemical activities of a toxoid of erythrogenic toxin type A.

A toxoid of erythrogenic toxin type A (ET A) was prepared by formaldehyde treatment. Already 15 min after exposure to formaldehyde in isoelectric focusing the ET A band at pH 5.2 shifted to a band at pH 4.5. In Ouchterlony double diffusion test ET A and its toxoid were found to be identical, in fused rocket immuno-electrophoresis a reaction of partial identity was seen. Formaldehyde treatment of ET A resulted in an apparent increase of electrophoretic mobility. In contrast to ET A, its toxoid is non-mitogenic, non-pyrogenic and has lost its ability to induce delayed type hypersensitivity. Binding of ET A toxoid to human peripheral lymphocytes is of the same magnitude as binding of gold-labelled ET A.

Animals↗
PubMed1988

Type 1 and 3 M-proteins of Streptococcus pyogenes: peptic extraction and fibrinogen binding properties.

The pepsin extraction of group A type 1 streptococci for the isolation of M protein fragments was studied at different pH values and at different time intervals. The extracts were compared by SDS PAGE and fused rocket immunoelectrophoresis. Type 1 M protein fragments were prepared in preparative scale by pepsin extraction of type 1 streptococci at pH 5.5 for 60 min. The fragments were separated by affinity chromatography on immobilized fibrinogen and finally purified for sequence studies by gel chromatography. Pepsin extraction of group A type 3 streptococci was also studied at different pH values. In contrast to type 1, the SDS PAGE pattern changed drastically in dependence on the pH. Affinity chromatography on immobilized fibrinogen is also effective in the separation of the pH 5.5 type 3 streptococcal pepsin extract.

Antigens, Bacterial↗
PubMed1988

CAMP-like phenomena of Vibrios.

The CAMP test was proposed by Lesmana and Rockhill (11) as a method to differentiate between classical V. cholerae (negative) and V. cholerae biotype El Tor (positive). Typical CAMP phenomena with a crescent-like zone of complete hemolysis were observed also with some strains of V. metschnikovii, V. parahaemolyticus, and V. anguillarum when the cultures were incubated anaerobically (mostly also at aerobic conditions). No CAMP reaction was seen with V. harveyi and V. costicola.

Aerobiosis↗
PubMed1988

Tissue cages for study of experimental streptococcal infection in rabbits. III. Influence of immunization with erythrogenic toxin type A (ET A) and its toxoid on subsequent infection with an ET A producing strain.

Purified erythrogenic toxin type A (ET A) and the corresponding toxoid, prepared by formalin treatment, were used for the immunization of rabbits (200 micrograms per rabbit). The impact of anti-erythrogenic toxin and toxoid immunity was investigated under the conditions of experimental infection with the ET A-producing Streptococcus pyogenes strain SF 130 (type 1). Whereas non of the immunized rabbits (n = 14) died after infection, 40% of nonimmunized animals did not survive (Table 1). The increase of the spleen weight after infection was significantly smaller in the immunized groups (Table 2). The immunized rabbits responded after infection with a significantly lower increase of fever which did not exceed 0.8 degree C (2 degrees C in infected non-immunized animals). Humoral antibodies to ET A were detected after immunization by means of ELISA. The challenge infection acted as a booster leading to a further increase of antibodies. The antibodies were found to be neutralizing the nonspecific mitogenicity of ET A in vitro in relation to the antibody titer. Cell-mediated immunity was tested in the lymphocyte transformation reaction with peripheral lymphocytes. The nonspecific mitogenicity of ET A, ET B, ET C and Con A was pronounced after immunization, whereas the nonimmunized rabbits responded to these antigens to a lower degree. The toxoid was found to be nonmitogenic. The altogether higher lymphocyte stimulation was also observed using spleen lymphocytes of immunized animals after infection.

Animals↗
PubMed1988

Visual evoked potentials of mildly mentally retarded and control children.

Visual evoked potentials (VEPs) were recorded from 25 10- to 13-year-old mildly mentally retarded children and compared with those from 31 control children of the same age-range. Correlations of VEPs with age were weak, but a relationship between VEPs and IQ was demonstrated for the control group. The retarded group had significantly longer latencies and higher amplitude peaks than the control group, with the differences occurring primarily over non-specific cortex and for secondary components. Analysis also showed that the retarded group were neurophysiologically heterogeneous. Since the same children had been analyzed earlier by quantitative EEG methods, comparisons are made with respect to these two methods of investigating brain function.

Adolescent↗