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Biomedical subjects

W Kasprzak

Publications and source records attributed to W Kasprzak.

At least 55 records · Page 3Linked to original sources

Naegleria fowleri in cooling waters of power plants.

Six strains of nonvirulent and three strains of virulent variants of Naegleria fowleri amoebae were isolated from the examined cooling water samples from 9 power plants. The virulent variants were obtained solely from effluents discharged from power plants with a closed-circuit cooling N. fowleri was not detected outside the reach of the thermal pollution. A disinfection of out-flowing cooling water seems to be an unnecessary investment in our climate. Warm discharge water should under no conditions be used directly for sports and recreational purposes.

Amoeba↗

Naegleria fowleri in thermally polluted waters).

Two complexes of lakes and canals supplying water for two electric power plants, their steam condensors and an adjoining river were investigated by means of culture methods for the presence of Naegleria fowleri in Poland in the period from 1974 to 1980. Sixty-four strains of N. fowleri were isolated, 13 isolates being virulent for mice when instilled intranasally. These strains were found in the steam condensor of the power station A and in waters polluted with warm water of this plant. Pathogenic N. fowleri strains occurred also in an adjoining river connected with the water system of the power plant. The results show the possible role of the steam condensor A as an incubator and regular source of pollution with pathogenic amoebae for its own system of cooling waters and even the adjoining river.

Amoeba↗

Electrophoretic characterization of small free-living Amoebae.

Disc electrophoresis was employed in an attempt to determine its usefulness as a means of differentiating and classifying some free-living species of amoebae. The Rp values and the percentage of similarity of the protein bands were used as taxonomic criteria. Some species of the genera Hartmanella and Naegleria were examined. The protein patterns of all species studied were highly reproducible. Four strains of Hartmannella castellanii showed an absolute identity to the original H. castellanii strain. One strain isolated by Neff and H. rhysodes were similar to all the other strains although their protein patten were not completely identical. On the other hand, H. culbertsoni, H. astronyxis and H. lammellipodia were different from all the other examined species of Hartmannella. Pathogenic N. fowleri-strains were identical to each other and were different in their protein patterns from non-pathogenic N. gruberi strain. The similarities and differences of protein bands of strains of ameobae and their correlations with other attributes were discussed. The results have shown that the disc electrophoresis appears useful in differentiating between species of Hartmannella and Naegleria.

Amoeba↗