Biomedical subjects
Publications and source records attributed to W Katz.
In selected patients who previously have undergone YAG laser ablation of the endometrium and who have demonstrated normal intrauterine architecture, pregnancy as a planned event may be a reasonable and safe option.
Two surface analytical spectroscopic techniques (namely, angular-dependent X-ray photoelectron spectroscopy and static secondary ion mass spectrometry) were used to characterize the nature and composition of the surface and near-surface layers formed on three biomedical polymers (namely, low-density polyethylene, expanded polytetrafluoroethylene, and a silicone elastomer) when exposed to Factor Xa in a buffer solution under adsorption conditions. It is shown that the results extracted from the spectroscopic measurements are consistent with each other. The clinical significance of the results is discussed in terms of screening biomedical polymers for use in fabricating cardiovascular system components.
Ten dyslexic adults (aged 33.5 +/- 7.3 years, nine men, one woman) and 10 age-, sex- and handedness-matched control subjects (aged 33.6 +/- 5.8 years) performed an auditory syllable discrimination task during 18-fluoro-2-deoxyglucose uptake, and then underwent positron emission tomographic scans. A second normal control group performed an analogous visual discrimination task. Dyslexic subjects experienced greater difficulty and made significantly more errors in performing the auditory task. There were no differences in brain metabolic rates in lateral cortical areas (frontal, parietal, temporal, and occipital lobes). A significant difference emerged in the medial temporal lobe, with dyslexic subjects having significantly higher absolute and relative brain metabolism along an anterior-posterior gradient than normal adults. These data support the hypothesis of altered cerebral processing of auditory stimuli in patients with dyslexia.
To investigate the issue of delay versus deviance in the language acquisition of language-impaired (LI) children, the order of acquisition of a set of linguistic structures and the relationship obtaining between one structure and another were examined in comprehension and production over a 5-year period in a group of LI and language-matched normal children. The results demonstrated a marked similarity between groups, both in the point at which mastery of individual structures was achieved and in the overall patterns of acquisition demonstrated. These data suggest that LI children are constructing grammars based on the same rules and principles as those of linguistically normal control subjects, and that their linguistic impairments may be principally processing, not representational, in nature.
An investigation was made into the extent and time course of anticipatory coarticulation in the speech of two normal and two anterior aphasic, German-speaking subjects. Both labial and velar coarticulation gestures were investigated. Subjects produced sentences containing target words contrasting in postconsonantal vowel rounding (e.g., [geli:ge]/[gely:gel]) and in nasality (e.g., [ti:de]/[ti:ne]). Speech kinematics were monitored by means of electromagnetic articulography. The data revealed that for correct productions, aphasic speakers' coarticulatory patterns were more highly variable than those of control subjects. These differences, however, were found chiefly for spatial displacement characteristics, while the temporal aspects of articulator movement necessary for anticipatory coarticulation appeared largely intact. Articulator mistiming did not appear to explain a small corpus of stop/nasal substitution errors produced by one of the aphasic speakers.
Microtubule organization in the cytoplasm is in part a function of the number and length of the assembled polymers. The intracellular concentration of tubulin could specify those parameters. Saccharomyces cerevisiae strains constructed with moderately decreased or increased copy numbers of tubulin genes provide an opportunity to study the cellular response to a steady-state change in tubulin concentration. We found no evidence of a mechanism for adjusting tubulin concentrations upward from a deficit, nor did we find a need for such a mechanism: cells with no more than 50% of the wild-type tubulin level were normal with respect to a series of microtubule-dependent properties. Strains with increased copies of both alpha- and beta-tubulin genes, or of alpha-tubulin genes alone, apparently did down regulate their tubulin levels. As a result, they contained greater than normal concentrations of tubulin but much less than predicted from the increase in gene number. Some of this down regulation occurred at the level of protein. These strains were also phenotypically normal. Cells could contain excess alpha-tubulin protein without detectable consequences, but perturbations resulting in excess beta-tubulin genes may have affected microtubule-dependent functions. All of the observed regulation of levels of tubulin can be explained as a response to toxicity associated with excess tubulin proteins, especially if beta-tubulin is much more toxic than alpha-tubulin.
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A detailed acoustic analysis was conducted of the speech production of a single patient presenting with the foreign accent syndrome subsequent to a left-hemisphere stroke in the subcortical white matter of the pre-rolandic and post-rolandic gyri at the level of the body of the lateral ventricle. It was the object of this research to determine those changes which contribute to the perception of a "foreign accent." A number of acoustic parameters were investigated, including features of consonant production relating to voice, place, and manner of articulation, vowel production relating to vowel quality and duration, and speech melody relating to fundamental frequency. The results indicated that many attributes which might have contributed to the foreign quality of the patient's speech were similar to those of normal English speakers. However, a number of critical elements involving consonant and vowel production and intonation were impaired. It was hypothesized that the acoustically anomalous features are linked to a common underlying deficit relating to speech prosody. It is suggested that the normal listener categorizes this speech pattern as a foreign accent because the anomalous speech characteristics, while not a part of the English phonetic inventory, reflect stereotypical features which are a part of the universal phonetic properties found in natural language.
The microtubules of mature nucleated erythrocytes are organized into a marginal band that is confined to a single plane at the periphery and that contains essentially the same number of microtubule profiles in each individual cell. Developing erythrocytes can be isolated in homogeneous and synchronously developing populations from chicken embryos. For these reasons, these cells offer a particularly accessible system for study of the pathway leading to a specific microtubule structure in a normal, terminally differentiated animal cell. Along this developmental course, striking changes occur in the properties of the microtubules. Between the postmitotic cell and the formation of the band, a novel arrangement is found: bundles of laterally associated microtubules in each cell, coursing through the cytoplasm but not confined to the periphery. The microtubule organizing centers evident at early stages disappear by the time the band forms. The microtubules in early cells are readily depolymerized by drugs, but that drug sensitivity is lost in the mature cells. The microtubule arrangement of mature cells is faithfully recapitulated after reversible depolymerization, while that of the immature cells is not. Finally, as the band forms, the microtubules and microfilaments increasingly become coaligned. In sum, the microtubules of immature cells have many properties in common with those of cultured cells, but during maturation those properties change. The results suggest that lateral interactions become increasingly important in stabilizing and organizing the microtubules. The properties of marginal band microtubules, and comparable properties of axonal microtubules, may reflect differences between the requirements for cytoskeletal structures of cycling cells and terminally differentiated cells.
Increased signal on T2-weighted magnetic resonance (MR) images has been demonstrated in skeletal muscle adjacent to neoplasms. Clinical significance of this sign was evaluated by reviewing MR images of 50 patients with musculoskeletal disorders. Increased signal in adjacent muscle was present in 22 patients, including 13 of 25 with primary or secondary malignancies of the musculoskeletal system. Biopsy specimens from the affected area in 11 of these 13 patients revealed edema (n = 8) more often than tumor invasion (n = 3). Nine of 25 patients with nonneoplastic processes had increased signal intensity in skeletal muscle because of infection, myositis, or hematoma. In patients who have not undergone previous surgery or radiation therapy and are without an inflammatory mass, increased signal intensity may be a useful indicator of malignancy.
We have reported a case of primary thrombosis of the axillary/subclavian vein, also known as the Paget-Schroetter syndrome, complicated by pulmonary embolus. It was successfully treated, along with the pulmonary embolus, with streptokinase infusion.
The visual abstraction procedure used in previous studies of declination was tested using 12 subjects who each fit the F0 contours of 19 spoken short simple sentences with baselines. These baselines were found to be poorly replicated the fitters. An objective all-points least-squares best-fit procedure was tested on this corpus and on a set of sentences that had been produced in both spontaneous and read speech by six speakers. The all-points linear regression line was a better descriptor of the F0 contours than either baselines or toplines. Declination did not always occur in these simple declarative sentences; there was more variation present in the F0 contours of sentences that had been uttered during spontaneous speech; 35% of the spontaneous sentences did not show declination; 45% of these sentences better fit the breath-group model. Their F0 contours could be described by a level all-points linear regression line followed by a falling terminal segment.
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A cell-bound bacteriocin was extracted from a Bacteroides fragilis BF-11 strain by treating the cells with a low-molarity buffer (0.01 M Tris-hydrochloride, pH 8.0). Sucrose osmotic shock experiments and ultrasonic lysis of whole cells indicated that the majority of the bacteriocin was located at the cell surface. Culture supernatants contained no significant bacteriocin activity. The bacteriocin was purified by DEAE-cellulose and Sephacryl S200 chromatography and had an apparent molecular weight of approximately 7,000. It was relatively heat stable and was inactivated by proteases. There was a delay of approximately 3.5 h before DNA, RNA, and protein synthesis were inhibited by the bacteriocin. Inhibition of macromolecular synthesis coincided with lysis of the susceptible indicator strain.
A method is described for the preparation of foetal calf serum from blood drawn by heart puncture from calf foetuses obtained from a local municipal abattoir. This method differs from that previously described in that the Seitz Supra EK filter pads used in preliminary purification steps have been abandoned. These pads contain significant amounts of pyrogenic bacterial lipopolysaccharides and attempts to depyrogenate them with sodium hypochlorite, hydrogen peroxide and 1% foetal calf serum were unsuccessful. The modified method uses pyrogen-free glass fibre and cellulose fibre filters and provides an entirely satisfactory serum as proved by negative Limulus amoebocyte lysate tests and the excellent growth of cells.
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