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Biomedical subjects

W Kohnle

Publications and source records attributed to W Kohnle.

8 recordsLinked to original sources
PubMed1980

The action of 1,25 (OH)2D3 on turnover kinetic, remodelling surfaces and structure of trabecular bone in chronic renal failure.

Fibroosteoclasia as well as osteoidosis are reduced by 1,25(OH)2D3 treatment if secondary hyperparathyroidism preexists. Fibrosteoclasia completely disappeared after 6 months therapy in some cases. 1,25(OH)2D3 has no or only a very slight effect on the disturbed mineralization in type II of renal bone disorder (osteoidosis only, no signs of secondary hyperparathyroidism). The appositional rate of the osteoblasts increases under 1,25(OH)2D3 treatment if serum PTH values are raised. 1,25(OH)2D3 seemed to have, in the chosen dosage of this study, a self-limiting effect by reducing the bone-forming cells, i.e. the osteoblasts. This state already represents an overtreatment of the underlying bone disease.

Adult↗
PubMed1979

Effect of hemoperfusion with coated and uncoated charcoal on human blood in vitro.

During 4 hours, in-vitro hemoperfusion of human blood was performed in 4 series of experiments: control, cellulose coated charcoal, polyacrylohydrogel coated charcoal and uncoated charcoal. In the control series little change on corpuscular elements and electrolytes was observed. In all hemoperfusion series there was little change of RBC and uniformly a marked fall of the granulocyte count. Platelet- and lymphocyte counts were significantly less affected by cellulose coated charcoal than by the other preparations. Coated and uncoated charcoal absorbed avidly calcium and glucose from the blood. By contrast the potassium level decreased moderately with polyacrylohydrogel coated and uncoated charcoal, whereas the opposite was observed with cellulose coated charcoal.

Blood Cell Count↗
PubMed1979

Hemodiafiltration using readily available equipment.

Hemodiafiltration as a blood purification method is a combination of hemodialysis and hemofiltration. With this procedure low molecular substances are predominantly cleared by diffusion while middle molecules are cleared mainly by convection. This report shows how hemodiafiltration can be done using readily available equipment: a regular dialysis machine, an "open membrane" and the COTRAL device. Treatment could be reduced to about 2/3 of dialysis time. With a TMP of 600 mmHg a filtration rate of 60 - 70 ml/min at a blood flow of 200 ml/min and a dialysate flow rate of 500 ml/min the following. Clearance values were obtained: Urea 140 ml/min, creatinine 128 ml/min and inulin 76 ml/min. Increasing the blood flow to 300 ml/min and dialysate flow to 1000 ml/min clearances were: urea 192 ml/min, creatinine 161 ml/min and inulin 91 ml/min. During a 3.5 hour treatment period max. 92.4 urea and 6.3 g creatinine and 36 mg B2 microglobulin were extracted. In our opinion this in our hand advantageous procedure deserves further study.

Blood Pressure↗
PubMed1978

[Antibody coated bacteria in the urine: a specific test for pyelonephritis (author's transl)].

Three groups of subjects have been studied: the first group with sterile urine, the second with significant bacteriuria without antibody-coated bacteria and the third with significant bacteriuria with antibody-coated. IgG, IgA, IgM and transferrin were determined by immunological methods (partigen plates). The antibody coating was determined by immunofluorescence. Subcultures were prepared from the urine samples containing bacteria. The bacteria obtained from the subcultures were then incubated with the original urine which had been sterilised by passing through a filter. These were then treated with AH-IgG containing fluorescein. Serum was taken from several patients and incubated with the bacteria from the subcultures. These were also treated with AH-IgG fluorescein. In no case could specific antibodies against the infecting organisms be found, although IgG in the urine were elevated and specific antibodies were present in the serum. This leads to the conclusion that specific antibodies are formed by direct tissue contact of the bacteria and that coating is not established in the urine itself.

Antibodies, Bacterial↗
PubMed1977

Acid base status during treatment of chronic uremia with diafiltration.

Treatment of chronic uremia by hemodiafiltration requires replacement of the filtrate. Using Ringer's solution alone, there is a depression of pH because of bicarbonate loss. To bring the acid base status back to normal, sodium lactate in increasing concentrations (283 mg% = 32 mM/1, 361 mg% = 40 mM/1; 462 mg% = 51 mM/1; 508 mg% = 57 mM/1) was added to the replacement fluid. The optimal concentration is 450 mg% (=50 mM/1) sodium lactate, provided the following conditions are fulfilled: (a) substitution after the filter; (b) mixing ratio of blood and substitution fluid 1:2. Using 12-15 liters of substitution fluid during a 5 hr treatment, the added lactate amounts to 60 g (=0.54 M). With continuous addition of lactate, the serum concentration of lactate is 3.5 times normal and the concentration of serum pyruvate 4 times normal. An excess lactate concentration, according to Huckabee [1,2], was thus not observed. The sieving coefficients were the following: sodium, potassium, urea, lactate, pyruvate, and phosphate 1; chloride greater then 1; calcium and protein less than 1. Serum osmolality fell, on the average, 9 mOsmol/1 during diafiltration.

Acid-Base Equilibrium↗
PubMed1975

[Localization of urinary-tract infection by demonstrating antibody-coated bacteria in urine (author's transl)].

The immunofluorescence test for demonstrating antibody-coated bacteria as a direct method of localizing urinary-tract infection was performed on 34 patients, results being compared with the Fairley test. Mid-stream urine contained at least 10(5) bacteria/ml in all patients. The direct localization method revealed upper urinary-tract infection (renal bacteriuria) in 18 patients, lower urinary-tract infection (cystitis) in eight. Presence of antibody-coated bacteria correlated well with renal bacteriuria, such antibody having been demonstrated in all of the eighteen patients with renal bacteriuria but in none of the eight with lower urinary-tract infection (cause located in the bladder). The test has proved to be highly sensitive method for differentiating between upper and lower urinary-tract infection.

Antibodies, Bacterial↗