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Biomedical subjects

W L Olszewski

Publications and source records attributed to W L Olszewski.

At least 181 records · Page 10Linked to original sources
PubMed1981

Specific migration of antigen-sensitive lymphocytes form the spleen to the site of allograft.

Specific spontaneous migration of antigen-sensitive splenocytes and thoracic duct lymphocytes from the transplanted spleen to the site of lymphocyte or skin allograft was studied in rats. Lack of specific recruitment of splenocytes in the allograft and relevant lymph nodes was found. Sensitized thoracic duct lymphocytes migrated specifically to the graft draining lymph nodes but not to the graft itself. The findings indicate that lymphocytes migrating from the spleen belong to the recirculating pool and are detained by sequestered antigen. The antigen-specific splenocytes have limited migratory properties and their function in the spleen seems to be devoted primarily to elimination of allo-antigens.

Animals↗
PubMed1981

Surgical pathophysiology of the lymphatic system.

The cannulation of lymph vessels by means of polyethylene Clay-Adams P 60 tube were through out in legs of 25 normal men. The lymph quantity, the contents of immunoglobulin and complement as well as cells and the pressure were measured. Rhythmic contractions of lymph vessels may be a major determinant of lymph flow. The pressure ranged between 20 and 50 mmHg. Leg lymph flow and protein concentration undergo major 24 hr variation dependent on the position of the body and the type of activity. At night lymph flow while in the rest is low. A continuous decrease in lymph protein concentration observed while in the upright position may be explained by the wash-out of proteins accumulated during periods in the horizontal position. The immune defence in the interstitial fluid and lymph of normal tissues seems to be weaker than in blood. Antibodies have definite biological properties. The contents of leucocyte and erythrocyte in human leg lymph is discussed.

Complement System Proteins↗
PubMed1981

Immunological characteristics of cells infiltrating sponge-matrix allografts in rats.

The morphological and functional characterization of cells infiltrating sponge-matrix allografts was done in rats. A higher percentage of esterase positive cells in the allografts already on day 1, with a gradual increase until day 7, was found. There was also a rise in percentage of esterase positive cells in peripheral blood of the allograft recipients. A higher percentage of phagocytic cells in the allografts on day 1, compared to syngeneic grafts, and a gradual rise during the ensuing six days were observed. These findings indicate the possibility of early recognition of alloantigen in the graft by monocytes and macrophages.

Animals↗
PubMed1980

Immunologic characteristics of human peripheral lymph cell populations.

Cell populations in afferent lymph of human leg were defined by surface characteristics and cytotoxic activity in 7 normal men and 9 patients with localized cancer. A higher percentage of E-rosette forming cells was found in lymph (78.5 and 83.0) than in blood (60.0 and 63.0 p less than 0.05). The percentages of lymph EA-RFC were 10.3 and 18.0, of EAC-RFC 13.1 and 8.0, of surface immunoglobulin carrying cells 3.0 and 3.1. In blood 20.6 and 18.0 percent of cells formed EA-rosettes, 23.0 and 15.6 EAC-rosettes, 5 and 9.5 contained surface immunoglobulins. The differences between lymph and blood EA- and EAC-RFC in normals were statistically significant (p less than 0.05). In cancer patients only lymph-blood differences for S Ig+ were significant (p less than 0.05). No significant differences were found between normals and cancer patients. In both groups, the natural cytotoxicity against K 562 cells was 6 times lower in lymph as compared to blood (p less than 0.05), the cytotoxicity in those with cancer was higher than in normals (p less than 0.05). The study indicates that B cells have a limited tendency toward leaving the blood circulation and migrating through tissues. Moreover natural killer cells do not seem to belong to the recirculating pool of lymphocytes.

Adult↗
PubMed1979

Microsurgical techniques in organ physiology and transplantation studies.

Experimental models for studies of physiology and pathology of different organs should be technically simple and reproducible, especially when large numbers of animals are required. Also uniform, often genetically identical, populations of animals are necessary to obtain reliable data. To meet these requirements studies should be performed on small animals. Modern microsurgical techniques help in solving these problems. The basic principles of microsurgical techniques and the microsurgical physiological and transplantation models have been described. Standardizing of techniques and models will allow better comparison of data from different centers.

Animals↗
PubMed1979

Intrinsic contractility of leg lymphatics in man. Preliminary communication.

Intralymphatic pressures were measured in subcutaneous trunks of 5 randomly selected healthy individuals. Rhythmic pulse waves of different frequency, amplitude of 1-33 mmHg, duration of 6-8 sec and steadily increasing mean pressure up to 50-120 mmHg were found, with subjects in the upright position and motionless. Coincidence of the rhythm of pulse waves with the rhythm of expression of drops of lymph from the cannula strongly suggests that pressures necessary to propel lymph were generated by the contractility of lymph vessel wall.

Adult↗
PubMed1978

Biological and biochemical characteristics of serum fraction effluent from xenografts and inhibiting leukocyte migration.

Serum effluent from the hyperacutely rejected rabbit-to-dog liver graft strongly inhibits migration of peripheral blood leukocytes. Fractionation of this serum on DEAE-Sephadex A-50 revealed the most active fraction to be that containing IgG. This finding supports our view that immune complexes formed during the hyperacute rejection of xenografts are mainly responsible for the inhibition of migration of peripheral blood leukocytes.

Animals↗
PubMed1978

Immune proteins and other biochemical constituents of peripheral lymph in patients with malignancy and postirradiation lymphedema.

Concentration of immunoglobulins and complement proteins were studied in a group of 33 patients with localized tumors and lymphoproliferative disorders. Generally, low levels have been found, in many cases below the lowest limit of the control group. The reductions in concentration were more pronounced in patients with lympho-proliferative disorders than with solid tumors. The most reduced were IgM, C1g and total complement hemolytic activity. In a group of 8 patients with lymphedema of lower extremity complicating therapy for uterine cancer an increase of IgM and IgA and decrease in hemolytic activity were found. This indicates to the existence of a chronic inflammatory process, typical for tissues deprived lymphatic outflow.

Adult↗
PubMed1978

Whole body localization of intravenously injected lymphoblasts in normal rats.

Total body distribution of IUDR labeled thoracic duct, mesenteric and peripheral lymph node blasts was investigated, after i.v. injection into normal syngeneic rats. Donors of immunoblasts were stimulated with SRBC 1 week before transfer of cells. Lymphoblasts, irrespective of the source of origin, accumulated preferentially in small intestine, lungs, skin and muscles. There was an evident difference in the whole body distribution pattern of injected thoracic duct and lymph node blasts, what points to the different behaviour of normally circulating immunoblasts. Only minor differences in distribution kinetics and sites of accumulation were found between mesenteric and peripheral blast cells. We put forward a hypothesis that immunoblasts behave like effector cells and their preferential localization in small gut, lungs, and skin may be mediated by antigens "physiologically" present on the surgace of tissue with direct contact with the external environment.

Animals↗