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Biomedical subjects

W L Ryan

Publications and source records attributed to W L Ryan.

At least 37 records · Page 2Linked to original sources
PubMed1972

Adenosine 3',5'-monophosphate and N 6 -2'-O-dibutyryl-adenosine 3',5'-monophosphate transport in cells.

Incubation of Strain L cells in 0.15 millimolar adenosine 3',5'-monophosphate (cyclic AMP) increase the intracellular level of cyclic AMP more than does incubation in dibutyryl cyclic AMP. The intracellular increase of cyclic AMP is greater with Strain L than with WI-38 cells. Similarly, cell division of Strain L cells is inhibited more by cyclic AMP. The experiments do not, support the belief that dibutyryl cyclic AMP is transported better than cyclic AMP.

Animals↗
PubMed1971

Amino Acid analysis and molecular weight determination of tetanus toxin.

A procedure for the isolation of tetanus toxin was developed. The toxin was precipitated with ammonium sulfate and further purified by use of molecular sieve chromatography and preparative disc gel electrophoresis. The degree of purification resulting from these fractionation procedures was monitored by analytical poly-acrylamide gel electrophoresis, immunodiffusion, and immunoelectrophoresis, all three of which demonstrated a single component. There was a 50-fold increase in specific activity between the crude culture filtrate and the final purified product, which had an average specific activity of 300 x 10(6) mouse minimal lethal doses per mg of N. A molecular weight of 140,000 was estimated by gel filtration on Bio-Gel P-200. A complete amino acid analysis of tetanus toxin is presented.

Journal Article↗
PubMed1970

Glycine inhibition of asparaginase.

Administration of either Escherichia coli asparaginase or guinea pig serum to C3H/HE mice with the 6C3HED lymphosarcoma is followed by depression of glycine in the tumor. This decrease in cellular glycine concentration does not occur in a tumor resistant to asparaginase. The inhibition of the lymphosarcoma by asparaginase can be reversed by intraperitoneal injection of asparagine or glycine. This reversal appears to be specific because lysine, threonine, serine, and aspartic acid were ineffective. Loss of cellular glycine may be more important than loss of asparagine because of the requirement for glycine in purine synthesis.

Animals↗
PubMed1970

Continuous-flow ultracentrifugation of canine distemper virus and infectious canine hepatitis virus.

The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.

Adenoviruses, Canine↗