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Biomedical subjects

W L Yau

Publications and source records attributed to W L Yau.

6 recordsLinked to original sources
PubMed1992

Myofibroblasts in hepatitis B related cirrhosis and hepatocellular carcinoma.

Peritoneal liver biopsy specimens from eight patients with hepatitis B associated cirrhosis, complicated by hepatocellular carcinoma, were studied for identification and localisation of myofibroblasts. The avidin-biotin peroxidase complex technique was used on paraffin wax sections, using monoclonal antibodies for actin and desmin, and ultrastructural examination was performed. Myofibroblasts were found in seven of the eight cirrhotic specimens and in all eight tumour specimens. They were identified in the fibrotic areas by the immunohistochemical technique, but ultrastructural examination disclosed their presence in the perisinusoidal space and between tumour cells.

Antibodies, Monoclonal↗
PubMed1990

Localisation of CD10 to biliary canaliculi by immunoelectron microscopical examination.

Common acute lymphoblastic leukaemia antigen (CALLA) was first characterised in lymphoid leukaemic cells. The antigen is present in different stages of lymphoid cell differentiation as well as in subsets of myeloid cells, and further studies have also shown its presence in non-lymphoid tissues. The recent cloning and sequencing of the gene permitted deduction of its amino acid sequence which is identical with the human membrane-associated enzyme, neutral endopeptidase. Strong immunostaining for CALLA was detected in the human liver with a canalicular pattern. Immunoelectron microscopy also confirmed that the antigen was localised only in the area of the bile canaliculi. Although the function of neutral endopeptidase in the canaliculi is unknown, this antigen may prove useful in the study of biliary function and diseases.

Antigens, CD↗
PubMed1985

A rapid method for reprocessing paraffin sections for diagnostic electron microscopy.

A rapid method for re-embedding paraffin sections into epoxy resin for diagnostic electron microscopy is described. The method requires a relatively shorter time than the traditional block retrieval technique and produces a reasonable retrieval technique and produces a reasonable quality of ultrastructure for diagnostic purposes.

Carcinoma, Squamous Cell↗
PubMed1984

Resin-slide for X-ray analysis of inorganic deposits on paraffin sections in the scanning electron microscope.

A preparation method for x-ray analysis of inorganic deposits on paraffin sections is described. It involves the use of resin-slides for mounting paraffin sections which are then dewaxed, carboncoated and examined in the SEM. The resin support, being much cheaper than the carbon planchet and having a perfectly flat surface, facilitates the adhesion of paraffin sections to it, and slides can be stored desiccated for retrospective study. The method also permits direct examination of sections under a light microscope to locate areas of interest. It is simple and useful for the analysis of inorganic inclusions in biological samples.

Elements↗